Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(12), P. 2568 - 2568
Published: Dec. 13, 2024
Butachlor
is
a
widely
utilized
acetamide
herbicide
noted
for
its
systemic
selectivity
against
pre-emergence
grass
weeds.
has
negative
effects
on
organisms
and
the
environment,
so
it
necessary
to
screen
degradation
strains.
In
this
investigation,
Bacillus
cereus
strain
DC-1
was
isolated
from
soil
persistently
exposed
butachlor.
Through
rigorous
single-factor
response
surface
analyses,
exhibited
notable
87.06%
efficiency
under
optimized
conditions
where
temperature
32.89
°C,
pH
7.29,
inoculum
concentration
5.18%.
It
further
hypothesized
by
LC-MS
that
pathway
of
butachlor
might
be
as
follows:
undergoes
initial
deoxygenation
catalyzed
dioxygenases
form
2-chloro-N-(2,6-diethylphenyl)-N-methylacetamide,
followed
N-demethylation
yielding
2-chloro-N-(2,6-diethylphenyl)
acetamide,
culminating
in
conversion
2,6-diethylphenol.
addition,
bioremediation
experiments
butachlor-contaminated
were
conducted.
The
results
show
could
degradable
99.23%
(100
mg·kg−1)
within
12
d,
sucrase,
cellulase,
urease
activities
are
promoted
bacteria.
And
through
high-throughput
sequencing,
concluded
able
influence
relative
abundance
certain
bacteria
soil,
make
microbial
community
develop
more
stable
beneficial
direction.
thus
represents
valuable
resource
realm
due
robust
efficacy,
favorable
characteristics,
ecological
restorative
capabilities,
underscoring
promising
role
soils.
Tobacco
(Nicotiana
tabacum)
black
shank
disease,
caused
by
Phytophthora
nicotianae,
is
a
significant
threat
to
tobacco
crops,
leading
severe
economic
losses.
Prolonged
use
of
agrochemicals
control
this
disease
has
prompted
the
exploration
eco-friendly
biological
strategies.
This
study
investigated
effects
Trichoderma
harzianum,
biocontrol
agent,
on
N.
tabacum
in
comparison
P.
focusing
growth,
biomass,
root
morphology
and
anatomy,
hormonal
changes,
osmotic
regulation.
T.
harzianum
significantly
enhanced
plant
biomass
accumulation,
system
development,
physiological
attributes
such
as
photosynthetic
pigment
levels
antioxidant
enzyme
activity.
In
contrast,
nicotianae
negatively
impacted
these
parameters,
inhibiting
growth
function.
Notably,
increased
proline
content
induced
resistance
mechanisms,
mitigating
stress
promoting
overall
health.
These
findings
highlight
potential
sustainable
solution
for
managing
while
improving
crop
productivity.
Frontiers in Microbiology,
Journal Year:
2024,
Volume and Issue:
15
Published: Oct. 16, 2024
Introduction
Maize
stalk
rot
(MSR),
caused
by
Fusarium
graminearum
,
is
the
most
serious
soil
borne
disease
in
maize
production,
seriously
affecting
yield
and
quality
worldwide.
Microbial
biocontrol
agents
are
best
means
of
controlling
MSR
reducing
use
chemical
fungicides,
such
as
Bacillus
spp.
Methods
results
In
study,
a
soil-isolated
strain
B105-8
was
identified
B.
velezensis
(accession
No.
PP325775.1
PP869695.1),
demonstrated
broad
spectrum
against
various
pathogens
causing
diseases,
which
effectively
controlled
MSR,
exhibited
high
control
efficacy
more
than
60%
growth-promoting
effect
pot
plant.
could
improve
urease
(S-UE),
invertase
(S-SC),
catalase
(S-CAT)
activities.
S-NP
activity
showed
an
initial
increase
with
peak
20,337
nmol/h/g,
followed
decrease,
but
remained
significantly
better
treatment
fungicides.
The
application
repaired
damage
F.
on
activity.
antifungal
compound
B-1,
extracted
from
B105-8,
purified
using
protein
purifier,
revealing
inhibitory
effects
.
Mass
spectrometry
analysis
indicated
potential
presence
C14
Bacillomycin,
C15
Iturin,
Mycosubtilin,
C17,
fengycin
B-1.
experiments,
5
μL/mL
concentration
B-1
69%
enhancing
root
elongation,
elevation,
fresh
weight.
At
10
μL/mL,
89.0
82.1%
inhibition
spore
production
mycelial
growth,
hyphal
deformities.
Discussion
This
study
presents
innovative
isolated
rhizosphere
cold
conditions
to
findings
highlight
remarkable
regional
adaptive
characteristics
this
strain,
making
it
excellent
candidate
fight
diverse
environments.
conclusion,
agent
for
MSR.
