NMR Spectroscopy for Metabolomics Research

Metabolites, Journal Year: 2019, Volume and Issue: 9(7), P. 123 - 123

Published: June 27, 2019

Over the past two decades, nuclear magnetic resonance (NMR) has emerged as one of three principal analytical techniques used in metabolomics (the other being gas chromatography coupled to mass spectrometry (GC-MS) and liquid with single-stage (LC-MS)). The relative ease sample preparation, ability quantify metabolite levels, high level experimental reproducibility, inherently nondestructive nature NMR spectroscopy have made it preferred platform for long-term or large-scale clinical metabolomic studies. These advantages, however, are often outweighed by fact that most techniques, including both LC-MS GC-MS, more sensitive than NMR, lower limits detection typically 10 100 times better. This review is intended introduce readers field NMR-based highlight advantages disadvantages It will also explore some unique strengths metabolomics, particularly regard isotope selection/detection, mixture deconvolution via 2D spectroscopy, automation, noninvasively analyze native tissue specimens. Finally, this a number emerging technologies strengthen its utility overcome inherent limitations applications.

Language: Английский

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Therapeutic Potential of Phenolic Compounds in Medicinal Plants—Natural Health Products for Human Health

Molecules, Journal Year: 2023, Volume and Issue: 28(4), P. 1845 - 1845

Published: Feb. 15, 2023

Phenolic compounds and flavonoids are potential substitutes for bioactive agents in pharmaceutical medicinal sections to promote human health prevent cure different diseases. The most common found nature anthocyanins, flavones, flavanones, flavonols, flavanonols, isoflavones, other sub-classes. impacts of plant phenolics on promoting diseases curing preventing antioxidant effects, antibacterial impacts, cardioprotective anticancer immune system promoting, anti-inflammatory skin protective effects from UV radiation. This work aims provide an overview phenolic as important sources medical application according recently published studies, well some interesting directions future research. keyword searches flavonoids, phenolics, tannins, coumarins, lignans, quinones, xanthones, curcuminoids, stilbenes, cucurmin, phenylethanoids, secoiridoids were performed by using Web Science, Scopus, Google scholar, PubMed. acids contain a carboxylic acid group addition the basic structure mainly divided into hydroxybenzoic hydroxycinnamic acids. Hydroxybenzoic based C6-C1 skeleton often bound small organic acids, glycosyl moieties, or cell structural components. Common include gallic, syringic, protocatechuic, p-hydroxybenzoic, vanillic, gentistic, salicylic Hydroxycinnamic C6-C3 also molecules such quinic glucose. main caffeic, p-coumaric, ferulic, sinapic

Language: Английский

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High‐Throughput Metabolomics by 1D NMR

Angewandte Chemie International Edition, Journal Year: 2018, Volume and Issue: 58(4), P. 968 - 994

Published: July 12, 2018

Metabolomics deals with the whole ensemble of metabolites (the metabolome). As one -omic sciences, it relates to biology, physiology, pathology and medicine; but are chemical entities, small organic molecules or inorganic ions. Therefore, their proper identification quantitation in complex biological matrices requires a solid ground. With respect for example, DNA, much more prone oxidation enzymatic degradation: we can reconstruct large parts mammoth's genome from specimen, unable do same its metabolome, which was probably largely degraded few hours after animal's death. Thus, need standard operating procedures, good skills sample preparation storage subsequent analysis, accurate analytical broad knowledge chemometrics advanced statistical tools, at least two metabolomic techniques, MS NMR. All these traditionally cultivated by chemists. Here focus on metabolomics standpoint restrict ourselves From point view, NMR has pros cons does provide peculiar holistic perspective that may speak future adoption as population-wide health screening technique.

Language: Английский

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Recent advances in the application of metabolomics for food safety control and food quality analyses

Critical Reviews in Food Science and Nutrition, Journal Year: 2020, Volume and Issue: 61(9), P. 1448 - 1469

Published: May 22, 2020

As one of the omics fields, metabolomics has unique advantages in facilitating understanding physiological and pathological activities biology, physiology, pathology, food science. In this review, based on developments analytical chemistry tools, cheminformatics, bioinformatics methods, we highlight current applications safety, authenticity quality, traceability. Additionally, combined use with other techniques for "foodomics" is comprehensively described. Finally, latest advances, practical challenges limitations, requirements related to application are critically discussed, providing new insight into analysis.

Language: Английский

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Metabolic retroconversion of trimethylamine N-oxide and the gut microbiota

Microbiome, Journal Year: 2018, Volume and Issue: 6(1)

Published: April 20, 2018

The dietary methylamines choline, carnitine, and phosphatidylcholine are used by the gut microbiota to produce a range of metabolites, including trimethylamine (TMA). However, little is known about use N-oxide (TMAO) this consortium microbes. A feeding study using deuterated TMAO in C57BL6/J mice demonstrated microbial conversion TMA, with uptake TMA into bloodstream its TMAO. Microbial activity necessary convert was suppressed antibiotic-treated mice, being taken up directly bloodstream. In batch-culture fermentation systems inoculated human faeces, growth Enterobacteriaceae stimulated presence Human-derived faecal caecal bacteria (n = 66 isolates) were screened on solid liquid media for their ability TMAO, metabolites spent analysed 1H-NMR. As vitro experiments, Enterobacteriaceae; these produced most from Caecal/small intestinal isolates Escherichia coli more than counterparts. Lactic acid increased amounts lactate when grown but did not large TMA. Clostridia (sensu stricto), bifidobacteria, coriobacteria significantly correlated production mixed system notable quantities pure culture. Reduction (predominantly Enterobacteriaceae) followed host intestine back hepatic enzymes an example metabolic retroconversion. influences metabolism depending isolation source taxon bacterium. Correlation metabolomic abundance data give true picture which members responsible converting TMA; only supplementing culture work additional metabolomics it possible increase our understanding bioconversions microbiota.

Language: Английский

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Navigating freely-available software tools for metabolomics analysis

Metabolomics, Journal Year: 2017, Volume and Issue: 13(9)

Published: Aug. 9, 2017

Language: Английский

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Microbiome and metabolome features of the cardiometabolic disease spectrum

Nature Medicine, Journal Year: 2022, Volume and Issue: 28(2), P. 303 - 314

Published: Feb. 1, 2022

Abstract Previous microbiome and metabolome analyses exploring non-communicable diseases have paid scant attention to major confounders of study outcomes, such as common, pre-morbid co-morbid conditions, or polypharmacy. Here, in the context ischemic heart disease (IHD), we used a design that recapitulates initiation, escalation response treatment over time, mirroring longitudinal would otherwise be difficult perform given protracted nature IHD pathogenesis. We recruited 1,241 middle-aged Europeans, including healthy individuals, individuals with dysmetabolic morbidities (obesity type 2 diabetes) but lacking overt diagnosis at three distinct clinical stages—acute coronary syndrome, chronic failure—and characterized their phenome, gut metagenome serum urine metabolome. found about 75% features distinguish from after adjustment for effects medication lifestyle are present exhibiting dysmetabolism, suggesting alterations might begin long before onset IHD. further categorized signatures related prodromal specific general each its subtypes de-escalation Discriminant analysis based on could better differentiate metabolically matched compared conventional risk markers, pointing pathophysiological relevance these features.

Language: Английский

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Frontiers of high-throughput metabolomics

Current Opinion in Chemical Biology, Journal Year: 2017, Volume and Issue: 36, P. 15 - 23

Published: Jan. 5, 2017

Language: Английский

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Recent Advances in NMR-Based Metabolomics

Analytical Chemistry, Journal Year: 2016, Volume and Issue: 89(1), P. 490 - 510

Published: Nov. 18, 2016

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTRecent Advances in NMR-Based MetabolomicsG. A. Nagana Gowda† and Daniel Raftery*†‡§View Author Information† ‡ †Northwest Metabolomics Research Center, Department of Anesthesiology Pain Medicine ‡Department Chemistry, University Washington, Seattle, Washington 98109, United States,§ Fred Hutchinson Cancer States*Phone: 206-543-9709. Fax: 206-616-4819. E-mail: [email protected]Cite this: Anal. Chem. 2017, 89, 1, 490–510Publication Date (Web):November 17, 2016Publication History Published online2 December 2016Published inissue 3 January 2017https://pubs.acs.org/doi/10.1021/acs.analchem.6b04420https://doi.org/10.1021/acs.analchem.6b04420review-articleACS PublicationsCopyright © 2016 American Chemical SocietyRequest reuse permissionsArticle Views4972Altmetric-Citations130LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated by Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence for given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Anatomy,Cancer,Metabolism,Metabolomics,Nuclear magnetic resonance spectroscopy Get e-Alerts

Language: Английский

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Focus on the glycerophosphocholine pathway in choline phospholipid metabolism of cancer

NMR in Biomedicine, Journal Year: 2019, Volume and Issue: 32(10)

Published: June 11, 2019

Activated choline metabolism is a hallmark of carcinogenesis and tumor progression, which leads to elevated levels phosphocholine glycerophosphocholine in all types cancer tested so far. Magnetic resonance spectroscopy applications have played key role detecting these phospholipid metabolites. To date, the majority cancer-related studies focused on Kennedy pathway, constitutes biosynthesis pathway for membrane phosphatidylcholine. Fewer more recent reported importance cancer. In this review article, we summarize literature with respect its biology detection by magnetic applications.

Language: Английский

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