Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of Group 1 Xanthomonas DOI
Daniel J. E. McKnight, Johanna Wong‐Bajracharya, Efenaide B. Okoh

et al.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2024, Volume and Issue: 74(3)

Published: March 27, 2024

Five bacterial isolates were isolated from Fragaria × ananassa in 1976 Rydalmere, Australia, during routine biosecurity surveillance. Initially, the results of biochemical characterisation indicated that these represented members genus Xanthomonas . To determine their species, further analysis was conducted using both phenotypic and genotypic approaches. Phenotypic involved MALDI-TOF MS BIOLOG GEN III microplates, which confirmed but did not allow them to be classified with respect species. Genome relatedness indices extensive phylogenetic a novel On basis minimal presence virulence-associated factors typically found genomes , we suggest are non-pathogenic. This conclusion supported by pathogenicity assay. findings, propose name rydalmerensis DAR 34855 T = ICMP 24941 as type strain.

Language: Английский

Epidemiology, diversity, and management of bacterial spot of tomato caused by Xanthomonas perforans DOI
Peter Abrahamian, Jeannie M. Klein-Gordon, Jeffrey B. Jones

et al.

Applied Microbiology and Biotechnology, Journal Year: 2021, Volume and Issue: 105(16-17), P. 6143 - 6158

Published: Aug. 1, 2021

Language: Английский

Citations

20
Xanthomonas oryzae Pv. oryzicola Response Regulator VemR Is Co-opted by the Sensor Kinase CheA for Phosphorylation of Multiple Pathogenicity-Related Targets DOI Creative Commons
Lulu Cai, Wenxiu Ma, Lifang Zou

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: June 9, 2022

Two-component systems (TCSs) (cognate sensor histidine kinase/response regulator pair, HK/RR) play a crucial role in bacterial adaptation, survival, and productive colonization. An atypical orphan single-domain RR VemR was characterized by the non-vascular pathogen Xanthomonas oryzae pv. oryzicola (Xoc) is known to cause leaf streak (BLS) disease rice. Xoc growth pathogenicity rice, motility, biosynthesis of extracellular polysaccharide (EPS), ability trigger HR non-host tobacco were severely compromised deletion mutant strain RΔvemR as compared wild-type RS105. Site-directed mutagenesis phosphotransfer experiments revealed that conserved aspartate (D56) residue within stand-alone phosphoacceptor receiver (REC) domain essential for phosphorelay regulatory activity VemR. Yeast two-hybrid (Y2H) co-immunoprecipitation (co-IP) data identified CheA HK co-opting phosphorylation. Affinity proteomics several downstream VemR-interacting proteins, such 2-oxoglutarate dehydrogenase (OGDH), DNA-binding SirA, flagellar basal body P-ring formation protein FlgA, Type 4a pilus retraction ATPase PilT, stress-inducible BaeS, septum site-determining MinD, cytoskeletal CcmA, III VI secretion system proteins HrpG Hcp, respectively. Y2H analyses corroborated interacted with OGDH, HrpG; thus, implicating multi-layered control diverse cellular processes including carbon metabolism, Physical interaction between suggested cross-talk CheA/VemR- HpaS/HrpG-mediated signal transduction events orchestrating hrp gene expression.

Language: Английский

Citations

12
Dysbiosis of a leaf microbiome is caused by enzyme secretion of opportunisticXanthomonasstrains DOI Creative Commons
Sebastian Pfeilmeier,

Anja Werz,

Marine Ote

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 10, 2023

Abstract Dysbiosis is characterized by a perturbed microbiota associated with host disease. In both plants and animals, the innate immune system contributes to maintain homeostasis in healthy organisms, NADPH oxidases playing crucial role. Arabidopsis thaliana , absence of oxidase RBOHD can lead an altered leaf microbiota, including enrichment opportunistic Xanthomonas pathogens. It currently unclear whether change occurs independently pathogens or caused latter, which virulence factors are essential for its lifestyle. Here, we found that strains secrete cocktail cell wall degrading enzymes via type-2 secretion (T2SS) degrade tissue promote growth during plant infection. Both disease severity degradation activity were increased rbohD compared Col-0 plants, attesting behaviour on compromised plants. Using gnotobiotic experiments synthetic bacterial community more than 100 commensal drop-in wildtype mutant revealed T2SS-dependent required shift composition. Overall, our data indicate single pathogen drive shifts, here damage leaves, creating environment specific bacteria thrive.

Language: Английский

Citations

7
Live tracking of a plant pathogen outbreak reveals rapid and successive, multidecade plasmid reduction DOI Creative Commons
Verónica Román-Reyna, Anuj Sharma,

Hannah Toth

et al.

mSystems, Journal Year: 2024, Volume and Issue: 9(2)

Published: Jan. 26, 2024

The geranium industry, valued at $4 million annually, faces an ongoing Xanthomonas hortorum pv. pelargonii (Xhp) pathogen outbreak. To track and describe the outbreak, we compared genome structure across historical globally distributed isolates. Our research revealed Xhp population has not had chromosome rearrangements since 1974 three distinct plasmids. In 2012, found all plasmids in individual However, 2022, co-integrated into one plasmid named p93. p93 retained putative fitness genes but lost extraneous genomic material. findings show that 2022 strain group of bacterial plant underwent a reduction. We also observed several species from different years, hosts, continents have similar to p93, possibly due shared agricultural settings. noticed parallels between efficiency reduction see millennia with obligate parasites increased niche specificity.

Language: Английский

Citations

2
Xanthomonas rydalmerensis sp. nov., a non-pathogenic member of Group 1 Xanthomonas DOI
Daniel J. E. McKnight, Johanna Wong‐Bajracharya, Efenaide B. Okoh

et al.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2024, Volume and Issue: 74(3)

Published: March 27, 2024

Five bacterial isolates were isolated from Fragaria × ananassa in 1976 Rydalmere, Australia, during routine biosecurity surveillance. Initially, the results of biochemical characterisation indicated that these represented members genus Xanthomonas . To determine their species, further analysis was conducted using both phenotypic and genotypic approaches. Phenotypic involved MALDI-TOF MS BIOLOG GEN III microplates, which confirmed but did not allow them to be classified with respect species. Genome relatedness indices extensive phylogenetic a novel On basis minimal presence virulence-associated factors typically found genomes , we suggest are non-pathogenic. This conclusion supported by pathogenicity assay. findings, propose name rydalmerensis DAR 34855 T = ICMP 24941 as type strain.

Language: Английский

Citations

2