Circadian Rhythms and Sleep in Drosophila melanogaster

Genetics, Journal Year: 2017, Volume and Issue: 205(4), P. 1373 - 1397

Published: March 30, 2017

The advantages of the model organism Drosophila melanogaster, including low genetic redundancy, functional simplicity, and ability to conduct large-scale screens, have been essential for understanding molecular nature circadian (∼24 hr) rhythms, continue be valuable in discovering novel regulators rhythms sleep. In this review, we discuss current these interrelated biological processes wider implications research. Clock genes period timeless were first discovered screens developed 1970s. Feedback on their own transcription forms core clock, accurately timed expression, localization, post-transcriptional modification, function is thought critical maintaining cycle. Regulators, several phosphatases kinases, act different steps feedback loop ensure strong rhythms. Approximately 150 neurons fly brain that contain components clock together translate intracellular cycling into rhythmic behavior. We how groups serve functions allowing clocks entrain environmental cues, driving behavioral outputs at times day, flexible responses conditions. neuropeptide PDF provides an important signal synchronize neurons, although details accomplishes are still being explored. Secreted signals from also influence other tissues. SLEEP is, part, regulated by which ensures appropriate timing sleep, but amount quality sleep determined mechanisms a homeostatic balance between wake. Flies useful identifying large set genes, molecules, neuroanatomic loci regulating amount. Conserved aspects regulation flies mammals include wake-promoting roles catecholamine neurotransmitters involvement hypothalamus-like regions, regions implicated less clear parallels. Sleep subject factors such as food availability, stress, social environment. beginning understand identified molecules interact with each other, environment, regulate researchers can take advantage increasing mechanistic behaviors, learning memory, courtship, aggression, loss impacts behaviors. thus remain tool both discovery deep

Language: Английский

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Molecular Genetic Analysis of Circadian Timekeeping in Drosophila

Advances in genetics, Journal Year: 2011, Volume and Issue: unknown, P. 141 - 173

Published: Jan. 1, 2011

Language: Английский

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Circadian neuron feedback controls the Drosophila sleep–activity profile

Nature, Journal Year: 2016, Volume and Issue: 536(7616), P. 292 - 297

Published: Aug. 1, 2016

Language: Английский

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Unexpected features of Drosophila circadian behavioural rhythms under natural conditions

Nature, Journal Year: 2012, Volume and Issue: 484(7394), P. 371 - 375

Published: April 1, 2012

Language: Английский

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Identification of a Circadian Output Circuit for Rest:Activity Rhythms in Drosophila

Cell, Journal Year: 2014, Volume and Issue: 157(3), P. 689 - 701

Published: April 1, 2014

Language: Английский

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A Conserved Bicycle Model for Circadian Clock Control of Membrane Excitability

Cell, Journal Year: 2015, Volume and Issue: 162(4), P. 836 - 848

Published: Aug. 1, 2015

Language: Английский

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Drosophila Ionotropic Receptor 25a mediates circadian clock resetting by temperature

Nature, Journal Year: 2015, Volume and Issue: 527(7579), P. 516 - 520

Published: Nov. 1, 2015

Language: Английский

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Synchronous Drosophila circadian pacemakers display nonsynchronous Ca ²⁺ rhythms in vivo

Science, Journal Year: 2016, Volume and Issue: 351(6276), P. 976 - 981

Published: Feb. 25, 2016

In Drosophila, molecular clocks control circadian rhythmic behavior through a network of ~150 pacemaker neurons. To explain how the network's neuronal properties encode time, we performed brainwide calcium imaging groups neurons in vivo for 24 hours. Pacemakers exhibited daily changes intracellular Ca(2+) that were entrained by environmental cues and timed clocks. However, these rhythms not synchronous, as each group its own phase activation. displayed associated with morning or evening locomotor activities occurred ~4 hours before their respective behaviors. Loss receptor neuropeptide PDF promoted synchrony waves. Thus, modulation is required to sequentially time outputs from synchronous pacemakers.

Language: Английский

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A Circadian Output Circuit Controls Sleep-Wake Arousal in Drosophila

Neuron, Journal Year: 2018, Volume and Issue: 100(3), P. 624 - 635.e4

Published: Sept. 27, 2018

Language: Английский

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Capturing continuous, long timescale behavioral changes in Drosophila melanogaster postural data

PLoS Computational Biology, Journal Year: 2025, Volume and Issue: 21(2), P. e1012753 - e1012753

Published: Feb. 3, 2025

Animal behavior spans many timescales, from short, seconds-scale actions to daily rhythms over hours life-long changes during aging. To access longer timescales of behavior, we continuously recorded individual Drosophila melanogaster at 100 frames per second for up 7 days a time in featureless arenas on sucrose-agarose media. We use the deep learning framework SLEAP produce full-body postural dataset 47 individuals resulting nearly 2 billion pose instances. identify stereotyped behaviors such as grooming, proboscis extension, and locomotion ethograms explore how flies' varies across day experiment. find distinct patterns all behaviors, adding specific information about trends different grooming modalities, extension duration, speed what is known D. circadian cycle. Using our holistic measurements that hour after dawn unique point pattern behavioral composition this tracks well with other indicators health fraction spend moving vs. resting. The method, data, analysis presented here give us new clearer picture revealing novel features hint unexplored underlying biological mechanisms.

Language: Английский

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