Substrates for Neuronal Cotransmission With Neuropeptides and Small Molecule Neurotransmitters in Drosophila

Frontiers in Cellular Neuroscience, Journal Year: 2018, Volume and Issue: 12

Published: March 23, 2018

It has been known for more than 40 years that individual neurons can produce one neurotransmitter and neuropeptides often are colocalized with small molecule neurotransmitters (SMNs). Over the much progress made in understanding functional consequences of cotransmission nervous system mammals. There also some excellent invertebrate models have revealed roles coexpressed SMNs increasing complexity, flexibility dynamics neuronal signaling. However, fly Drosophila there surprisingly few studies on cotransmission, although is ample evidence colocalization neuroactive compounds CNS, based both traditional techniques novel single cell transcriptome analysis. With hope to trigger interest initiating studies, this review summarizes what about circuits where different colocalized. Coexistence substances recorded neuron types such as neuroendocrine cells, interneurons, sensory cells motor neurons. Some highlighted here well established analysis learning memory, circadian clock networks regulating rhythmic activity sleep, olfaction, nociception, feeding, metabolic homeostasis, diuretic functions, reproduction developmental processes. One emerging trait broad role short neuropeptide F presynaptic facilitation a number circuits. This discusses relevance coexisting peptides intestine. Based recent transcriptomics data, it likely systems discussed just fraction total set occurs Drosophila. Thus, systematic search further anatomically defined near future.

Language: Английский

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The Drosophila circuitry of sleep–wake regulation

Current Opinion in Neurobiology, Journal Year: 2017, Volume and Issue: 44, P. 243 - 250

Published: March 31, 2017

Language: Английский

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Sleep homeostasis regulated by 5HT2b receptor in a small subset of neurons in the dorsal fan-shaped body of drosophila

eLife, Journal Year: 2017, Volume and Issue: 6

Published: Oct. 6, 2017

Our understanding of the molecular mechanisms underlying sleep homeostasis is limited. We have taken a systematic approach to study neural signaling by transmitter 5-hydroxytryptamine (5-HT) in drosophila. generated knockout and knockin lines for Trh, 5-HT synthesizing enzyme all five receptors, making it possible us determine their expression patterns investigate functional roles. Loss 5HT1a or 5HT2b gene decreased time whereas loss Trh diminished rebound after deprivation. small subset of, probably single pair neurons dorsal fan-shaped body (dFB) functionally essential: elimination from these led homeostasis. Genetic ablation dFB impaired results shown that serotonergic specific required regulation

Language: Английский

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A Neural Network for Wind-Guided Compass Navigation

Neuron, Journal Year: 2020, Volume and Issue: 107(5), P. 924 - 940.e18

Published: July 17, 2020

Spatial maps in the brain are most accurate when they linked to external sensory cues. Here, we show that compass Drosophila is direction of wind. Shifting wind rightward rotates as if fly were turning leftward, and vice versa. We describe mechanisms several computations integrate information into compass. First, an intensity-invariant representation computed by comparing left-right mechanosensory signals. Then, signals reformatted reduce coding biases inherent peripheral mechanics, cues brought same circular coordinate system represents visual self-motion Because incorporates both cues, it should enable navigation under conditions where no single cue consistently reliable. These results how local can be transformed a global, multimodal, abstract space.

Language: Английский

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Temporal calcium profiling of specific circadian neurons in freely moving flies

Proceedings of the National Academy of Sciences, Journal Year: 2017, Volume and Issue: 114(41)

Published: Sept. 26, 2017

Significance Monitoring neuronal activity in freely moving, behaving animals is a holy grail of neuroscience. Here we present noninvasive tool that links the calcium profiles specific fly neurons to real-time behavior. Optogenetic manipulation two groups circadian indicates they drive sleep or locomotor activity. The patterns are also distinct and couple well sleep/activity phases sleep-promoting appear more active when flies initiate daytime sleep, whereas activity-promoting fire strongly coincident with evening peak. This new approach complementary electrophysiological recording GCaMP imaging, especially for small organisms behavorial paradigms which these traditional methods not practical.

Language: Английский

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