Time- and cell-dependent atypia and cell death are caused by progressive deficiency in DNA replication DOI Creative Commons
Alex Y. Lin,

Georgia K. Thomas,

K.C. Ang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: Jan. 3, 2021

Abstract Pleiotropy caused by single-gene mutations is common and poorly understood. A zebrafish null mutant of DNA polymerase α subunit B, huli hutu ( hht) , evolves a complex pleiotropy associated with damage S phase arrest across multiple organ systems over 5-7 days, including nuclear atypia, cellular feature in human cancers pre-cancers, gastrointestinal organs, fragmentation the eye brain. The pleiotropic pattern hht phenotypes explained progressive loss wild-type maternal pola2 function homozygous embryos whose mRNA becomes undetectable 24 hours post-fertilization (hpf). Inhibition synthesis aphidicolin or hydroxyurea from hpf phenocopied . These results are consistent model which time-sensitive, reduced capacity for cell death fast-replicating cells, atypia tissues fewer larger cells.

Language: Английский

Skin cells undergo asynthetic fission to expand body surfaces in zebrafish DOI
Keat Ying Chan, Ching‐Cher Sanders Yan, Hsiao-Yuh Roan

et al.

Nature, Journal Year: 2022, Volume and Issue: 605(7908), P. 119 - 125

Published: April 27, 2022

Language: Английский

Citations

34
Pattern regulation in a regenerating jellyfish DOI Creative Commons
Chiara Sinigaglia, Sophie Péron,

Jeanne Eichelbrenner

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Sept. 7, 2020

Jellyfish, with their tetraradial symmetry, offer a novel paradigm for addressing patterning mechanisms during regeneration. Here we show that an interplay between mechanical forces, cell migration and proliferation allows jellyfish fragments to regain shape functionality rapidly, notably by efficient restoration of the central feeding organ (manubrium). Fragmentation first triggers actomyosin-powered remodeling restores body umbrella shape, causing radial smooth muscle fibers converge around ‘hubs’ which serve as positional landmarks. Stabilization these hubs, associated expression Wnt6 , depends on configuration adjoining fiber ‘spokes’. Stabilized hubs presage site manubrium blastema, whose growth is Wnt/β-catenin dependent fueled both long-range recruitment. Manubrium morphogenesis modulated its connections gastrovascular canal system. We conclude in regenerating emerges mainly from local interactions, triggered directed process.

Language: Английский

Citations

43
Thyroid hormone regulates proximodistal patterning in fin rays DOI Creative Commons

Melody Harper,

Yinan Hu, Joan Donahue

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(21)

Published: May 15, 2023

Processes that regulate size and patterning along an axis must be highly integrated to generate robust shapes; relative changes in these processes underlie both congenital disease evolutionary change. Fin length mutants zebrafish have provided considerable insight into the pathways regulating fin size, yet signals underlying remained less clear. The bony rays of fins possess distinct proximodistal axis, reflected location ray bifurcations lengths segments, which show progressive shortening axis. Here, we thyroid hormone (TH) regulates aspects caudal rays, regardless size. TH promotes distal gene expression patterns, coordinating segment with skeletal outgrowth This distalizing role for is conserved between development regeneration, all (paired medial), Danio species as well distantly related medaka. During regenerative outgrowth, acutely induces Shh-mediated bifurcation. Zebrafish multiple nuclear receptors, found unliganded Thrab-but not Thraa or Thrb-inhibits formation features. Broadly, results demonstrate morphology regulated independently from size-instructive signals. Modulating size-either through metabolism other hormone-independent pathways-can shift ways recapitulate diversity nature.

Language: Английский

Citations

14
Beyond Adult Stem Cells: Dedifferentiation as a Unifying Mechanism Underlying Regeneration in Invertebrate Deuterostomes DOI Creative Commons
C. Ferrario, Michela Sugni, Ildikó Somorjai

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2020, Volume and Issue: 8

Published: Oct. 20, 2020

The diversity of regenerative phenomena seen in adult metazoans, as well their underlying mechanistic bases, are still far from being comprehensively understood. Reviewing both ultrastructural and molecular data, the present work aims to showcase increasing relevance invertebrate deuterostomes, i.e., echinoderms, hemichordates, cephalochordates tunicates, invaluable models study cellular aspects regeneration. Our comparative approach suggests a fundamental contribution local dedifferentiation -rather than mobilization resident undifferentiated stem cells- an important mechanism contributing regeneration these groups. Thus, elucidating origins, recruitment fate cells, signals underpinning tissue regrowth regeneration-competent will provide foundation for future research tackling relatively limited abilities vertebrates, with clear applications medicine.

Language: Английский

Citations

33
Appendage-resident epithelial cells expedite wound healing response in adult zebrafish DOI
Fiorency Santoso, Marco P. de Leon, Wei‐Chen Kao

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(16), P. 3603 - 3615.e4

Published: July 16, 2024

Language: Английский

Citations

4
Mechanical waves identify the amputation position during wound healing in the amputated zebrafish tailfin DOI
Marco P. de Leon, Fu-Lai Wen,

Giovanni J. Paylaga

et al.

Nature Physics, Journal Year: 2023, Volume and Issue: 19(9), P. 1362 - 1370

Published: June 19, 2023

Language: Английский

Citations

8
Positional Memory in Vertebrate Regeneration: A Century's Insights from the Salamander Limb DOI
Leo Otsuki, Elly M. Tanaka

Cold Spring Harbor Perspectives in Biology, Journal Year: 2021, Volume and Issue: 14(6), P. a040899 - a040899

Published: Oct. 4, 2021

Leo Otsuki and Elly M. Tanaka Research Institute of Molecular Pathology, 1030 Vienna, Austria Correspondence: leo.otsuki{at}imp.ac.at

Language: Английский

Citations

20
Molecular basis for positional memory and its reprogrammability in limb regeneration DOI Creative Commons
Leo Otsuki,

SA Plattner,

Yuka Taniguchi‐Sugiura

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 28, 2023

Abstract Upon limb amputation in salamanders, anterior and posterior connective tissue cells form distinct signalling centres that together fuel successful regeneration. The molecular properties distinguish prior to injury, which enable them initiate different after amputation, are not known. These identities, crucial for regeneration, were thought be established during development persist through successive regeneration cycles as positional memory. However, the nature of these memory states whether identities can engineered have remained outstanding questions. Here, we identify a positive feedback mechanism encoding identity axolotl limb, used newly encode regenerative cells. Posterior express residual levels bHLH transcription factor Hand2 from this is priming molecule necessary sufficient establish Shh centre amputation. During feeds back reinforces expression nearby sustained following safeguarding memory, while shut off. As consequence Hand2-Shh system, differentially susceptible alteration. stabilised against anteriorisation their poises trigger loop. In contrast, reprogrammed: transient exposure causes gain lasting competence . way, an opportunity entry point re-write Our results implicate stability explain why more easily altered one direction (anterior posterior) than other. Because modifying changes outputs cells, our findings wider implications engineering.

Language: Английский

Citations

7
Through veiled mirrors: Fish fins giving insight into size regulation DOI
Matthew P. Harris, Jacob M. Daane, Jennifer Lanni

et al.

Wiley Interdisciplinary Reviews Developmental Biology, Journal Year: 2020, Volume and Issue: 10(4)

Published: April 23, 2020

Abstract Faithful establishment and maintenance of proportion is seen across biological systems provides a glimpse at fundamental rules scaling that underlie development evolution. Dysregulation observed in range human diseases growth disorders, indicating proper an essential component normal anatomy physiology. However, when viewed through evolutionary lens, shifts the regulation relative are one most striking sources morphological diversity among organisms. To date, mechanisms via which specified maintained remain unclear. Through application powerful experimental, genetic molecular approaches, teleost fin has provided effective model to investigate scaling, size, vertebrate This article categorized under: Establishment Spatial Temporal Patterns > Regulation Size, Proportion, Timing Adult Stem Cells, Tissue Renewal, Regeneration Comparative Development Evolution Organ Diversity

Language: Английский

Citations

20
Positional information modulates transient regeneration-activated cell states during vertebrate appendage regeneration DOI
Augusto Ortega Granillo,

Daniel Zamora,

Robert R. Schnittker

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 31, 2024

Summary Injury is a common occurrence in the life of organisms. Because extent damage cannot be predicted, injured organisms must determine how much tissue needs to restored. It known that amputation position determines regeneration speed amputated appendages regeneration-competent animals. Yet, it not clear positional information conveyed during regeneration. Here, we investigated dynamics regenerating caudal fins African killifish ( Nothobranchius furzeri ). We report position-specific, differential modulation spatial distribution, duration, and magnitude proliferation. Regenerating profiled by single cell RNA sequencing identified Transient Regeneration-Activated Cell State (TRACS) amplified match given position. located this TRACS basal epidermis found them express components modifiers extracellular matrix (ECM). propose role for these cells transducing blastema remodeling ECM. Highlights Amputation changes tissue-wide proliferation response Transcriptional compartmentalization relative injury type Regeneration deploys States Prediction: transduced ECM

Language: Английский

Citations

1