Genome sequence and cell biological toolbox of the highly regenerative, coenocytic green feather alga Bryopsis

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(2), P. 1091 - 1111

Published: April 20, 2024

Green feather algae (Bryopsidales) undergo a unique life cycle in which single cell repeatedly executes nuclear division without cytokinesis, resulting the development of thallus (>100 mm) with characteristic morphology called coenocyte. Bryopsis is representative coenocytic alga that has exceptionally high regeneration ability: extruded cytoplasm aggregates rapidly seawater, leading to formation protoplasts. However, genetic basis biology remains poorly understood. Here, we present high-quality assembly and annotation genome sp. (90.7 Mbp, 27 contigs, N50 = 6.7 14 034 protein-coding genes). Comparative genomic analyses indicate genes encoding BPL-1/Bryohealin, aggregation-promoting lectin, are heavily duplicated Bryopsis, whereas homologous absent other ulvophyceans, suggesting capability Bryopsis. possesses >30 kinesins but only myosin, differs from green have multiple types myosin genes. Consistent this biased motor toolkit, observed bidirectional motility chloroplasts was dependent on microtubules not actin Most required for cytokinesis plants including those SNARE or kinesin superfamily. Nevertheless, crucial initiation (NACK/Kinesin-7II) hardly expressed part thallus, possibly underlying lack portion. The sequence lays foundation experimental macroalgae.

Language: Английский

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Light quality affects chlorophyll biosynthesis and photosynthetic performance in Antarctic Chlamydomonas

Photosynthesis Research, Journal Year: 2025, Volume and Issue: 163(1)

Published: Jan. 20, 2025

Language: Английский

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The genomic insights of intertidal adaptation in Bryopsis corticulans

New Phytologist, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Many marine green algae thrive in intertidal zones, adapting to complex light environments that fluctuate between low underwater and intense sunlight. Exploring their genomic bases could help comprehend the diversity of adaptation strategies response environmental pressures. Here, we developed a novel practical strategy assemble high-confidence algal genomes sequenced high-quality genome Bryopsis corticulans, an zone macroalga Bryopsidales order Chlorophyta originated 678 million years ago. Comparative analyses revealed previously overlooked whole duplication event closely related species, Caulerpa lentillifera. A total 100 genes were acquired through horizontal gene transfer, including homolog cryptochrome photoreceptor CRY gene. We also found all four species studied lack key photoprotective (LHCSR, PsbS, CYP97A3, VDE) involved xanthophyll cycle energy-dependent quenching processes. elucidated expansion light-harvesting antenna biosynthesis pathways for siphonein siphonaxanthin B. corticulans likely contribute its conditions. Our study unraveled underlying special genetic basis Bryopsis' environments, advancing our understanding plant adaptive evolution.

Language: Английский

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Restoration innovation: Fusing microbial memories to engineer coral resilience

One Earth, Journal Year: 2025, Volume and Issue: 8(3), P. 101193 - 101193

Published: March 1, 2025

Language: Английский

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Distribution of Conchocelis of Red Algae Bangia sp. in the Nacreous Layer of Akoya Pearl Oyster Pinctada fucata Shell

Marine Biotechnology, Journal Year: 2025, Volume and Issue: 27(3)

Published: April 16, 2025

Language: Английский

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Multiple techniques point to oxygenic phototrophs dominating the Isopora palifera skeletal microbiome

Coral Reefs, Journal Year: 2021, Volume and Issue: 40(2), P. 275 - 282

Published: Feb. 15, 2021

Language: Английский

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Effects of Ocean Warming on the Underexplored Members of the Coral Microbiome

Integrative and Comparative Biology, Journal Year: 2022, Volume and Issue: 62(6), P. 1700 - 1709

Published: March 6, 2022

Abstract The climate crisis is one of the most significant threats to marine ecosystems. It leading severe increases in sea surface temperatures and frequency magnitude heatwaves. These changing conditions are directly impacting coral reef ecosystems, which among biodiverse ecosystems on Earth. Coral-associated symbionts particularly affected because summer heatwaves cause bleaching—the loss endosymbiotic microalgae (Symbiodiniaceae) from tissues, starvation death. Symbiodiniaceae bacteria have been extensively studied context change, especially terms community diversity dynamics. However, data other microorganisms their response change scarce. Here, we review current knowledge how increasing affect understudied coral-associated such as archaea, fungi, viruses, protists than Symbiodiniaceae, well microbe-microbe interactions. We show that coral-microbe symbiosis equilibrium at risk under predicted future argue conservation initiatives should include microbe-focused approaches.

Language: Английский

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Evolution of the regulatory subunits for the heteromeric acetyl-CoA carboxylase

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1914)

Published: Sept. 29, 2024

The committed step for de novo fatty acid (FA) synthesis is the ATP-dependent carboxylation of acetyl-coenzyme A catalysed by acetyl-CoA carboxylase (ACCase). In most plants, ACCase a multi-subunit complex orthologous to prokaryotes. However, unlike prokaryotes, plant and algal orthologues are comprised both catalytic additional dedicated regulatory subunits. Novel subunits, biotin lipoyl attachment domain-containing proteins (BADC) carboxyltransferase interactors (CTI) (both three-gene families in

Language: Английский

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The trace metal economy of the coral holobiont: supplies, demands and exchanges

Biological reviews/Biological reviews of the Cambridge Philosophical Society, Journal Year: 2022, Volume and Issue: 98(2), P. 623 - 642

Published: Nov. 22, 2022

The juxtaposition of highly productive coral reef ecosystems in oligotrophic waters has spurred substantial interest and progress our understanding macronutrient uptake, exchange, recycling among holobiont partners (host coral, dinoflagellate endosymbiont, endolithic algae, fungi, viruses, bacterial communities). By contrast, the contribution trace metals to physiological performance and, turn, functional ecology reef-building corals remains unclear. holobiont's metal economy is a network supply, demand, exchanges upheld by cross-kingdom symbiotic partnerships. Each partner unique requirements that are central their biochemical functions metabolic stability holobiont. Organismal homeostasis determine ability adjust fluctuating supplies heterogeneous environments. This review details for core biological processes describes how key sustaining complex nutritional symbioses Specifically, we discuss contribute compatibility, cope with stress, thereby organismal fitness distribution. Beyond cycling, outline dynamic nature availability environmental can be influenced variability abiotic factors (e.g. temperature, light, pH, etc.). Climate change will have profound consequences on further intensify myriad stressors influence survival. Lastly, suggest future research directions necessary impacts spanning subcellular levels, which inform nutrient cycling more broadly. Collectively, this cross-scale elucidation role allow us improve forecasts function.

Language: Английский

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Fine‐scale mapping of physicochemical and microbial landscapes of the coral skeleton

Environmental Microbiology, Journal Year: 2023, Volume and Issue: 25(8), P. 1505 - 1521

Published: March 23, 2023

The coral skeleton harbours a diverse community of bacteria and microeukaryotes exposed to light, O2 pH gradients, but how such physicochemical gradients affect the microbiome remains unclear. In this study, we employed chemical imaging pH, hyperspectral reflectance spatially resolved taxonomic inferred functional characterization explore links between microenvironment in reef-building corals Porites lutea Paragoniastrea benhami. environment was more stable deep skeleton, diversity evenness bacterial increased with skeletal depth, suggesting that stratified along gradients. bulk low habitat, whereas varied from 6-9 depth. Physicochemical explained β-diversity communities, layers showed peaks had higher relative abundance endolithic algae, reflecting link abiotic composition. Our study physicochemical, microbial landscapes provides new insights into involvement microbes holobiont metabolism.

Language: Английский

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