Deep sea treasures - insights from museum archives shed light on coral microbial diversity within deepest ocean ecosystems DOI Creative Commons
Francesco Ricci, William Leggat, Marisa M. Pasella

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 5, 2023

Abstract Deep sea benthic habitats are low productivity ecosystems that host an abundance of organisms within the Cnidaria phyla. The technical limitations and high cost deep surveys have made exploring environments biology inhabit them challenging. In spite widespread recognition Cnidaria's environmental importance in these ecosystems, microbial assemblage its role coral functioning only been studied for a few water corals. Here we explored prokaryotic diversity corals by recovering nucleic acids from museum archive specimens. Firstly, amplified sequenced V1-V3 regions 16S rRNA gene specimens held archival storage utilised to shed light on associated with seven families collected depth Coral Sea (depth range 1,309 2,959 metres) Southern Ocean 1,401 2,071 habitats. Most surprisingly, Cyanobacteria sequences were consistently six out both sampling locations, suggesting bacteria potentially ubiquitous members microbiome cold organisms. Additionally, show might benefit symbiotic associations chemosynthetic including nitrite, carbon monoxide sulfur oxidizers. Consistent previous studies, bacterial phyla Proteobacteria, Verrucomicrobia, Planctomycetes Acidobacteriota dominated community sea. We also genomes genus Mycoplasma , which identified as three families, finding evidence may aid immune system. Importantly our results retain components can provide new insights into microbiomes (and other organisms), well microbes writ large ecosystems.

Language: Английский

Coral Skeleton Dwelling Endolithic Algae: Ostreobium and Its Biology DOI
Kshitij Tandon,

Juntong Hu,

Marisa M. Pasella

et al.

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 41 - 46

Published: Jan. 1, 2025

Language: Английский

Citations

1
Chemosynthesis: a neglected foundation of marine ecology and biogeochemistry DOI
Francesco Ricci, Chris Greening

Trends in Microbiology, Journal Year: 2024, Volume and Issue: 32(7), P. 631 - 639

Published: Jan. 30, 2024

Language: Английский

Citations

7
Multiparameter Sensing of Oxygen and pH at Biological Interfaces via Hyperspectral Imaging of Luminescent Sensor Nanoparticles DOI Creative Commons
Zsófia Bognár, Maria Moßhammer, Kasper Elgetti Brodersen

et al.

ACS Sensors, Journal Year: 2024, Volume and Issue: 9(4), P. 1763 - 1774

Published: April 12, 2024

Chemical dynamics in biological samples are seldom stand-alone processes but represent the outcome of complicated cascades interlinked reaction chains. In order to understand these and how they correlate, it is important monitor several parameters simultaneously at high spatial temporal resolution. Hyperspectral imaging a promising tool for this, as provides broad-range spectral information each pixel, enabling use multiple luminescent indicator dyes, while providing on sample structures optical properties. this study, we first characterized pH- O2-sensitive dyes incorporated different polymer matrices sensor nanoparticles provide library (hyperspectral) chemical imaging. We then demonstrate successful combination pH-sensitive dye (HPTS(DHA)3), an (PtTPTBPF), two reference (perylene TFPP), multiparameter complex natural such green algal biofilms (Chlorella sorokiniana) seagrass leaves (Zostera marina) with background fluorescence. discuss system-specific challenges limitations our approach further optimization possibilities. Our study illustrates hyperspectral read-out can now be applied samples, alignment structures.

Language: Английский

Citations

5
Modeling the radiative, thermal and chemical microenvironment of 3D scanned corals DOI Creative Commons
Swathi Murthy, Cristian Picioreanu, Michael Kühl

et al.

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 10

Published: May 22, 2023

Reef building corals are efficient biological collectors of solar radiation and consist a thin stratified tissue layer spread over light scattering calcium carbonate skeleton surface that together construct complex three dimensional (3D) colony structures forming the foundation coral reefs. They exhibit vast diversity structural forms to maximize photosynthesis their dinoflagellate endosymbionts (Symbiodiniaceae), while simultaneously minimizing photodamage, offer resistance hydrodynamic stress, reduce attack by predators increase prey capture heterotrophic feeding. The symbiosis takes place in presence dynamic gradients light, temperature chemical species affected interaction incident irradiance water flow with colony. We developed multiphysics modelling approach simulate microscale spatial distribution O 2 fragment its morphology determined 3D scanning techniques. Model results compared well measurements under similar conditions. model enabled us infer effect on internal environment experienced endosymbionts, as combined contribution ciliary movement distributions coral.

Language: Английский

Citations

5
Reconciling the variability in the biological response of marine invertebrates to climate change DOI Creative Commons
Zoe Dellaert, Hollie M. Putnam

Journal of Experimental Biology, Journal Year: 2023, Volume and Issue: 226(17)

Published: Sept. 1, 2023

ABSTRACT As climate change increases the rate of environmental and frequency intensity disturbance events, selective forces intensify. However, given complicated interplay between plasticity selection for ecological – thus evolutionary outcomes, understanding proximate signals, molecular mechanisms role history becomes increasingly critical eco-evolutionary forecasting. To enhance accuracy our forecasting, we must characterize signals at a level resolution that is relevant to organism, such as microhabitat it inhabits its intracellular conditions, while also quantifying biological responses these in appropriate cells tissues. In this Commentary, provide historical context some long-standing challenges global biology constrain capacity forecasting using reef-building corals focal model. We then describe examples mismatches scales external relative sensors signal transduction cascades initiate maintain cellular responses. Studying scale crucial because are basis acclimation changing conditions potential ‘memory’ prior or through mechanisms. challenge field, outline unresolved questions suggest approaches align experimental work with an organism's perception environment; aspects discussed respect human interventions.

Language: Английский

Citations

4
Deep sea treasures - Insights from museum archives shed light on coral microbial diversity within deepest ocean ecosystems DOI Creative Commons
Francesco Ricci, William Leggat, Marisa M. Pasella

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(5), P. e27513 - e27513

Published: March 1, 2024

Deep sea benthic habitats are low productivity ecosystems that host an abundance of organisms within the Cnidaria phylum. The technical limitations and high cost deep surveys have made exploring environments biology inhabit them challenging. In spite widespread recognition Cnidaria's environmental importance in these ecosystems, microbial assemblage its role coral functioning only been studied for a few water corals. Here, we explored diversity corals by recovering nucleic acids from museum archive specimens. Firstly, amplified sequenced V1–V3 regions 16S rRNA gene specimens, then utilized generated sequences to shed light on associated with seven families collected depth Coral Sea (depth range 1309 2959 m) Southern Ocean 1401 2071 habitats. Surprisingly, Cyanobacteria were consistently six out both sampling locations, suggesting bacteria potentially ubiquitous members microbiome cold Additionally, show might benefit symbiotic associations chemosynthetic including nitrite, carbon monoxide sulfur oxidizers. Consistent previous studies, bacterial phyla Proteobacteria, Verrucomicrobia, Planctomycetes Acidobacteriota dominated community sea. We also genomes genus Mycoplasma, which identified as specimens three families, finding evidence may aid immune system. Importantly our results retain components can provide new insights into microbiomes (and other organisms), well microbes writ large ecosystems.

Language: Английский

Citations

0
Deep sea treasures - insights from museum archives shed light on coral microbial diversity and functioning within deepest ocean ecosystems DOI Creative Commons
Francesco Ricci, William Leggat, Marisa M. Pasella

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: May 16, 2023

Abstract Deep sea benthic habitats are low productivity ecosystems that host an abundance of organisms within the Cnidaria phyla. The technical limitations and high cost deep surveys have made exploring environments biology inhabit them challenging. In spite widespread recognition Cnidaria's environmental importance in these ecosystems, microbial assemblage its role coral functioning only been studied for a few water corals. Here we explored prokaryotic diversity corals by recovering nucleic acids from museum archive specimens. Firstly, amplified sequenced V1-V3 regions 16S rRNA gene specimens held archival storage utilised to shed light on associated with seven families collected depth Coral Sea (depth range 1,309 2,959 metres) Southern Ocean 1,401 2,071 habitats. Most surprisingly, Cyanobacteria were consistently six out both sampling locations, suggesting bacteria potentially ubiquitous members microbiome cold organisms. Consistent previous studies, show bacterial phyla Proteobacteria, Verrucomicrobia, Planctomycetes Acidobacteriota dominate community sea. We also genomes genus Mycoplasma , which identified as three families, finding evidence may aid immune system. Importantly our results retain components can provide new insights into microbiomes (and other organisms), well microbes writ large ecosystems.

Language: Английский

Citations

0
Deep sea treasures - insights from museum archives shed light on coral microbial diversity within deepest ocean ecosystems DOI Creative Commons
Francesco Ricci, William Leggat, Marisa M. Pasella

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 5, 2023

Abstract Deep sea benthic habitats are low productivity ecosystems that host an abundance of organisms within the Cnidaria phyla. The technical limitations and high cost deep surveys have made exploring environments biology inhabit them challenging. In spite widespread recognition Cnidaria's environmental importance in these ecosystems, microbial assemblage its role coral functioning only been studied for a few water corals. Here we explored prokaryotic diversity corals by recovering nucleic acids from museum archive specimens. Firstly, amplified sequenced V1-V3 regions 16S rRNA gene specimens held archival storage utilised to shed light on associated with seven families collected depth Coral Sea (depth range 1,309 2,959 metres) Southern Ocean 1,401 2,071 habitats. Most surprisingly, Cyanobacteria sequences were consistently six out both sampling locations, suggesting bacteria potentially ubiquitous members microbiome cold organisms. Additionally, show might benefit symbiotic associations chemosynthetic including nitrite, carbon monoxide sulfur oxidizers. Consistent previous studies, bacterial phyla Proteobacteria, Verrucomicrobia, Planctomycetes Acidobacteriota dominated community sea. We also genomes genus Mycoplasma , which identified as three families, finding evidence may aid immune system. Importantly our results retain components can provide new insights into microbiomes (and other organisms), well microbes writ large ecosystems.

Language: Английский

Citations

0