Time of day and genotype sensitivity adjust molecular responses to temperature stress in sorghum DOI Creative Commons
Titouan Bonnot, Impa M. Somayanda, S. V. Krishna Jagadish

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 10, 2023

Abstract Sorghum is one of the four major C4 crops that are considered to be tolerant environmental extremes. shows distinct growth responses temperature stress depending on sensitivity genetic background. About half transcripts in sorghum exhibit diurnal rhythmic expressions emphasizing significant coordination with environment. However, an understanding how molecular dynamics contribute genotype-specific context time day not known. We examined whether and impact gene expression cold-sensitive heat-sensitive genotypes. found highly influencing responses, which can explained by most thermo-responsive genes. This effect more pronounced thermo-tolerant genotypes, suggesting a stronger regulation and/or circadian clock. Genotypic differences were mostly observed average levels, but we identified groups genes regulated time-of-day manner. These include transcriptional regulators several members Ca 2+ -binding EF-hand protein family. hypothesize variation these between genotypes may responsible for contrasting sensitivities vs susceptible varieties. findings offer new opportunity selectively target specific efforts develop climate-resilient based their genotype stress.

Language: Английский

Crosstalk between salicylic acid signaling and the circadian clock promotes an effective immune response DOI Creative Commons

Olivia J. P. Fraser,

Samantha J. Cargill, Steven H. Spoel

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 21, 2023

Abstract The rotation of Earth creates a cycle day and night, leading to predictable changes in environmental conditions. circadian clock synchronizes an organism with these alters their physiology anticipation. Prediction the probable timing pathogen infection enables plants prime immune system without wasting resources or sacrificing growth. Here, we explore relationship between hormone salicylic acid (SA), Arabidopsis. We found that SA altered rhythmicity through receptor master transcriptional coactivator, NPR1. Reciprocally, gates SA-induced expression NPR1-dependent genes. Furthermore, gene CCA1 is essential for immunity major bacterial plant Pseudomonas syringae . These results reveal new interactions signaling which produce effective response. Understanding how why response linked crucial working towards improved crop productivity.

Language: Английский

Citations

1
Investigating Circadian Gating of Temperature Responsive Genes DOI

Rachel I. Strout,

Calum A. Graham, Antony N. Dodd

et al.

Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 213 - 225

Published: Jan. 1, 2024

Language: Английский

Citations

0
Cold-induced degradation of core clock proteins implements temperature compensation in the Arabidopsis circadian clock DOI Creative Commons
Akari E Maeda,

Hiromi Matsuo,

Tomoaki Muranaka

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(39)

Published: Sept. 27, 2024

The period of circadian clocks is maintained at close to 24 hours over a broad range physiological temperatures due temperature compensation length. Here, we show that the quantitative control core clock proteins TIMING OF CAB EXPRESSION 1 [TOC1; also known as PSEUDO-RESPONSE REGULATOR (PRR1)] and PRR5 crucial for in Arabidopsis thaliana . prr5 toc1 double mutant has shortened higher temperatures, resulting weak compensation. Low ambient reduces amounts TOC1. In low-temperature conditions, TOC1 interact with LOV KELCH PROTEIN 2 (LKP2), component E3 ubiquitin ligase Skp, Cullin, F-box (SCF) complex. lkp2 mutations attenuate low temperature–induced decrease TOC1, mutants display longer only lower temperatures. Our findings reveal maintains its length despite fluctuations through temperature- LKP2 -dependent abundance.

Language: Английский

Citations

0
Circadian and diel regulation of photosynthesis in the bryophyte Marchantia polymorpha DOI Open Access

David Cuitun‐Coronado,

Hannah Rees, Joshua Colmer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Jan. 12, 2022

Abstract Circadian rhythms are 24-hour biological cycles that align metabolism, physiology and development with daily environmental fluctuations. Photosynthetic processes governed by the circadian clock in both flowering plants some cyanobacteria, but it is unclear how extensively this conserved throughout green lineage. We investigated contribution of regulation to aspects photosynthesis Marchantia polymorpha, a liverwort diverged from early evolution land plants. First, we identified M. polymorpha photosynthetic biochemistry, measured using two approaches (delayed fluorescence, PAM fluorescence). Second, light-dark synchronize phase 24 h , whereas phases different thalli desynchronize under free running conditions. This might also be due masking underlying cycles. Finally, used pharmacological approach identify chloroplast translation necessary for control light harvesting polymorpha. infer well-conserved amongst terrestrial

Language: Английский

Citations

1
Time of day and genotype sensitivity adjust molecular responses to temperature stress in sorghum DOI Creative Commons
Titouan Bonnot, Impa M. Somayanda, S. V. Krishna Jagadish

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 10, 2023

Abstract Sorghum is one of the four major C4 crops that are considered to be tolerant environmental extremes. shows distinct growth responses temperature stress depending on sensitivity genetic background. About half transcripts in sorghum exhibit diurnal rhythmic expressions emphasizing significant coordination with environment. However, an understanding how molecular dynamics contribute genotype-specific context time day not known. We examined whether and impact gene expression cold-sensitive heat-sensitive genotypes. found highly influencing responses, which can explained by most thermo-responsive genes. This effect more pronounced thermo-tolerant genotypes, suggesting a stronger regulation and/or circadian clock. Genotypic differences were mostly observed average levels, but we identified groups genes regulated time-of-day manner. These include transcriptional regulators several members Ca 2+ -binding EF-hand protein family. hypothesize variation these between genotypes may responsible for contrasting sensitivities vs susceptible varieties. findings offer new opportunity selectively target specific efforts develop climate-resilient based their genotype stress.

Language: Английский

Citations

0