Evolution of chemosensory tissues and cells across ecologically diverseDrosophilids DOI Creative Commons
Gwénaëlle Bontonou, Bastien Saint‐Leandre, Tane Kafle

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 15, 2023

Abstract Chemosensory systems display exceptional variation between species, but little is known about how the evolution of gene expression and cell types contribute to this diversity. We have generated transcriptomes for five chemosensory tissues across six ecologically diverse Drosophila species integrated their analyses with single-cell datasets address these questions. The has been predominantly shaped by stabilizing selection, several thousand genes nevertheless evolved changes in each tissue. Phylogenetic differentially expressed revealed strong evidence that driven a combination cis -regulatory composition evolution. also found chemosensory-related families undergone pervasive level numerous species-specific gains/losses. Follow-up experiments chemoreceptors novel patterns tissue cellular likely sensory differences. Finally, are sexes uncovered extensive Among rapid changes, we discovered D. melanogaster- specific excess male-biased its forelegs identified muscle cells as primary source dimorphism. Together, our provide new insights understanding evolutionary key at both global individual levels.

Language: Английский

SfMBP: A novel microbial binding protein and pattern recognition receptor in the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) DOI

Bang‐Xian Zhang,

Fangfang Liu,

Feng Liu

et al.

Developmental & Comparative Immunology, Journal Year: 2024, Volume and Issue: 154, P. 105142 - 105142

Published: Feb. 2, 2024

Language: Английский

Citations

4
Larval microbiota primes the Drosophila adult gustatory response DOI Creative Commons
Martina Montanari, Gérard Manière, Martine Berthelot‐Grosjean

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 13, 2024

Abstract The survival of animals depends, among other things, on their ability to identify threats in surrounding environment. Senses such as olfaction, vision and taste play an essential role sampling living environment, including microorganisms, some which are potentially pathogenic. This study focuses the mechanisms detection bacteria by Drosophila gustatory system. We demonstrate that peptidoglycan (PGN) forms cell wall triggers immediate feeding aversive response when detected system adult flies. Although we ppk23+ Gr66a+ neurons necessary transduce fly PGN, they very different roles process. Time-controlled functional inactivation vivo calcium imaging while required flies directly PGN signal, must be larvae allow future adults become sensitive. Furthermore, respond bacterial is lost hatch from reared under axenic conditions. Recolonization germ-free larvae, but not adults, with a single species, Lactobacillus brevis , sufficient restore PGN. Our data genetic environmental characteristics make competent certain sensory stimuli

Language: Английский

Citations

4
Fungi Associated with Other Organisms: Diversity, Interactions and Ecological Importance DOI Creative Commons
Luiz Augusto Adorno Soares Costa,

Flavia Helena Aires Sousa,

Evelise Leis Carvalho

et al.

IntechOpen eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Fungi play pivotal roles in ecosystems by forming diverse associations with other organisms, including plants, animals and microorganisms, along certain bacteria. These interactions range from mutualistic relationships, such as mycorrhizal facilitating nutrient uptake to parasitic causing disease the hosts. also engage commensal where one organism benefits without harming or significantly benefiting other. The ecological importance of these is profound, influencing cycling, biodiversity, ecosystem stability. For instance, lichens, composed fungi algae cyanobacteria, are pioneer species harsh environments contribute soil formation. Understanding dynamics fungal organisms crucial for management conservation efforts. Moreover, exploring molecular mechanisms underlying can offer insights into biotechnological applications, agriculture medicine, harnessing potential sustainable solutions various fields.

Language: Английский

Citations

0
Chemosensory protein 16 has an immune function and participates in host-pathogen interaction in Galleria mellonella infected with Pseudomonas entomophila DOI Creative Commons
Jakub Kordaczuk, Michał Sułek, Paweł Mak

et al.

Virulence, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 28, 2025

Chemosensory protein 16 was identified in the hemolymph of Galleria mellonella as a with an amount increasing after oral infection 10^3 CFU Pseudomonas entomophila, and decreasing higher dose (10^5 CFU) bacteria. The expression CSP16 gene occurred fat body gut correlated changes level hemolymph. inhibited P. entomophila growth concentration range from 0.15 to 6 nM. Additionally, showed bactericidal activity against Bacillus thuringiensis, Escherichia coli 2–18 μM, but only presence protease inhibitors, otherwise it degraded by extracellular proteases secreted entomophila. We demonstrated that related its ability perforate bacterial cellular membranes dose-dependent manner. antimicrobial properties this were also confirmed use Atomic Force Microscopy, which significant topology different cell surfaces. Finally, when injected vivo into G. larvae one hour more survivors observed at particular time-points. Taking account immune putative bind bacteria-derived compounds, possible function host-pathogen interaction is discussed.

Language: Английский

Citations

0
Knockdown of a chemosensory protein disrupts soil-guided behavior of a subterranean larval pest DOI

Paula Lizana,

Camila Muñoz, Ana Mutis

et al.

Journal of Insect Physiology, Journal Year: 2025, Volume and Issue: unknown, P. 104793 - 104793

Published: March 1, 2025

Language: Английский

Citations

0
Chromosome‐level genome assembly of Dendrothrips minowai and genomic analysis highlights distinct adaptations to high polyphenols in tea plants DOI
Chunli Xiu, Dehai Zhao, Jiahui Zhang

et al.

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 24, 2025

Abstract BACKGROUND Dendrothrips minowai Priesner, a significant pest in tea‐producing regions of Asia, particularly China, damages tea plants ( Camellia sinensis ) by feeding on their tender leaves rich polyphenols. Seven assembled genomes from the order Thysanoptera are currently available. RESULTS This study presents first chromosome‐level genome assembly D. generated PacBio Revio, Oxford Nanopore Technologies, MGI, and Hi‐C technology. The measures 350.11 Mb with 1269 contigs contig N50 536.34 Kb scaffold 16.86 Mb, organized across 19 chromosomes. A total 16 730 protein‐coding genes were identified, 92.28% functionally annotated. phylogenetic analysis reveals that diverged approximately 103.2 million years ago, preceding all reported Thripidae species. Comparative genomic identified 12 expanded 172 contracted gene families , linked to host plant metabolite processing detoxification enzymes. Additionally, oligophagous thrips, Stenchaetothrips biformis possess fewer chemosensory (gustatory receptors, odorant ionotropic proteins, binding proteins) (P450s, carboxyl/cholinesterases, UDP‐glycosyltransferases) than polyphagous species Frankliniella occidentalis Thrips palmi ). Interestingly, exhibits an expansion ABC transporter families, especially ABCG ABCC, which is likely essential for detoxifying high polyphenol content plants. CONCLUSION provides another sequence thrips species, enriches data further studies evolution, adaptation, novel control strategies thrips. © 2025 Society Chemical Industry.

Language: Английский

Citations

0
Fungus–insect interactions beyond bilateral regimes: the importance and strategy to outcompete host ectomicrobiomes by fungal parasites DOI
Hong Song, Yanlei Sun, Haimin Chen

et al.

Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 74, P. 102336 - 102336

Published: June 13, 2023

Language: Английский

Citations

8
Genetics and Infection Biology of the Entomopathogenic Fungi DOI
Hong Song, Junmei Shang, Yanlei Sun

et al.

Springer eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 309 - 331

Published: Jan. 1, 2024

Language: Английский

Citations

2
Engineered fungus containing a caterpillar gene kills insects rapidly by disrupting their ecto- and endo-microbiomes DOI Creative Commons
Hong Song,

Han‐Chun Gao,

Haimin Chen

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Aug. 7, 2024

Similar to the physiological importance of gut microbiomes, recent works have shown that insect ectomicrobiotas can mediate defensive colonization resistance against fungal parasites infect via cuticle penetration. Here we show engineering entomopathogenic fungus Metarhizium robertsii with a potent antibacterial moricin gene from silkworms substantially enhances ability kill mosquitos, locusts, and two Drosophila species. Further use melanogaster as an infection model, quantitative microbiome analysis reveals engineered strains designed suppress cuticular bacteria additionally disrupt microbiomes. An overgrowth harmful such opportunistic pathogens Providencia species is detected accelerate death. In support, antimicrobial genes in fly fat bodies guts indicates topical infections result compromise intestinal immune responses. addition providing innovative strategy for improving potency mycoinsecticides, our data solidify both ecto- endo-microbiomes maintaining wellbeing.

Language: Английский

Citations

2
A bacterial-like Pictet–Spenglerase drives the evolution of fungi to produce β-carboline glycosides together with separate genes DOI Creative Commons
Xinlin Li, Yanlei Sun, Ying Yin

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(30)

Published: July 19, 2023

Diverse β-carboline (βC) alkaloids are produced by microbes, plants, and animals with myriad bioactivities drug potentials. However, the biosynthetic mechanism of βCs remains largely elusive, especially regarding hydroxyl glucosyl modifications βCs. Here, we report presence bacterial-like Pictet–Spenglerase gene Fcs1 in entomopathogenic Beauveria fungi that can catalyze biosynthesis βC skeleton. The overexpression bassiana led to identification six methyl glycosides, termed bassicarbosides (BCSs) A–F. We verified cytochrome P450 (CYP) genes adjacent cannot oxidize Alternatively, separated CYP684B2 family Fcs2 was identified hydroxylation together its cofactor Fcs3 . functional homologue is only present Fcs1- containing highly similar connected yet nonfunctional CYP. Both evolved quicker than those from without homologues. Finally, paired methyl/glucosyl transferase were mediate production BCSs hydroxy-βCs. All these functionally located on different chromosomes , which contrast typical content-clustered feature fungal clusters (BGCs). also found selectively contributed infection insect species. Our findings shed light including a hydroxylase. results this study propose an evolving process BGC formation following horizontal transfer bacterial fungi.

Language: Английский

Citations

4