Evolution of gene expression across brain regions in behaviorally divergent deer mice DOI Open Access
Andreas F. Kautt, Jenny Chen, Caitlin L. Lewarch

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 28, 2023

Abstract The evolution of innate behaviors is ultimately due to genetic variation likely acting in the nervous system. Gene regulation may be particularly important because it can evolve a modular brain-region specific fashion through concerted action cis - and trans -regulatory changes. Here, investigate transcriptional its regulatory basis across brain, we perform RNA sequencing (RNA-Seq) on ten brain subregions two sister species deer mice ( Peromyscus maniculatus P. polionotus ) – which differ range behaviors, including their social system F 1 hybrids. We find that most gene expression distinguishes subregions, followed by species. Interspecific differential (DE) pervasive (52–59% expressed genes), whereas number DE genes between sexes modest overall (∼3%). Interestingly, identity varies considerably regions. Much this modularity divergence, while 43% were consistently assigned same class (e.g., conserved, -, or divergence), similar more different classes. Together, these results highlight differences divergence key explain how contribute astonishing diversity animal behaviors.

Language: Английский

Evolution of gene expression across brain regions in behaviourally divergent deer mice DOI Creative Commons
Andreas F. Kautt, Jenny Chen, Caitlin L. Lewarch

et al.

Molecular Ecology, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 23, 2024

Abstract The evolution of innate behaviours is ultimately due to genetic variation likely acting in the nervous system. Gene regulation may be particularly important because it can evolve a modular brain‐region specific fashion through concerted action cis ‐ and trans ‐regulatory changes. Here, investigate transcriptional its regulatory basis across brain, we perform RNA sequencing (RNA‐Seq) on ten brain subregions two sister species deer mice ( Peromyscus maniculatus P. polionotus )—which differ range behaviours, including their social system—and F 1 hybrids. We find that most gene expression distinguishes subregions, followed by species. Interspecific differential (DE) pervasive (52–59% expressed genes), whereas number DE genes between sexes modest overall (~3%). Interestingly, identity varies considerably regions. Much this modularity divergence, while 43% were consistently assigned same class (e.g. conserved, or divergence), similar more different classes. Together, these results highlight differences divergence which key explain how contribute astonishing diversity animal behaviours.

Language: Английский

Citations

8
A Functional and Non-Homuncular Representation of the Larynx in the Primary Motor Cortex of Mice, a Vocal Non-Learner DOI Creative Commons
César D. M. Vargas,

Rajvi K. Agravat,

Elena N. Waidmann

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 6, 2024

Summary Vocalization is a complex behavior ranging from fully innate to advanced vocal learning. Vocal learning species possess primary motor cortex (M1) region that makes direct projections brainstem neurons, which are thought facilitate and fine modulation of vocalizations. Surprisingly, similar, albeit sparse, projection M1 was found in mice. Mice produce ultrasonic vocalizations (USV) appear be mostly innate. Modulation these USVs impacted by lesions this region, but genetic ablation the leads few, if any, changes USVs. It remained unclear whether has any control over organ non-learning species. In current study, we stimulation different parts mice can generate contractions laryngeal jaw muscles, with latencies suggestive both indirect neurons. Viral tracing reveals single- double-labeled populations cortical neurons simultaneously innervate laryngeal, jaw, forelimb circuits. Chemical reveal an anterolateral orofacial regulates number syllables sequences. Our results provide evidence non-learner some influence on musculature, consistent continuum hypothesis They also representations muscles for behaviors across mouse more intermixed than previously considered. We discuss how impact hypotheses evolution organization.

Language: Английский

Citations

4
Encoding of Arousal and Physical Characteristics in Audible and Ultrasonic Vocalizations of Mongolian Gerbil Pups Testing Common Rules for Mammals DOI Creative Commons
Yara Silberstein, Felix Felmy, Marina Scheumann

et al.

Animals, Journal Year: 2023, Volume and Issue: 13(16), P. 2553 - 2553

Published: Aug. 8, 2023

In mammals, common rules for the encoding of arousal and physical characteristics sender are suggested based on a similar vocal production apparatus. this study, we want to investigate what extent vocalizations developing Mongolian gerbil pups fulfill these rules. We recorded 28 in four developmental stages using separation paradigm, induce different levels. For low arousal, pup was placed an arena isolated from its siblings parents; high additionally stressed through simulation predator. An unsupervised cluster analysis revealed three call types: ultrasonic (USV), audible (ADV), transitions between both (USV-ADV). The USV USV-ADV rate showed age-dependent decrease, contrasting increase ADVs. Vocal correlates were found USVs However, pattern cues differed types only partly confirmed mammals. Our results show that divergent patterns do not differ species but also within species, indicating coding can be shaped by socio-ecological factors or type specific mechanisms.

Language: Английский

Citations

8
SqueakOut: Autoencoder-based segmentation of mouse ultrasonic vocalizations DOI Creative Commons
Gustavo M. Santana, Marcelo O. Dietrich

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 23, 2024

Mice emit ultrasonic vocalizations (USVs) that are important for social communication. Despite great advancements in tools to detect USVs from audio files the recent years, highly accurate segmentation of spectrograms (i.e., removing noise) remains a significant challenge. Here, we present new dataset 12,954 annotated explicitly labeled mouse USV segmentation. Leveraging this dataset, developed SqueakOut, lightweight (4.6M parameters) fully convolutional autoencoder achieves high accuracy supervised spectrograms, with

Language: Английский

Citations

1
The Neurobiology of Parenting and Infant-Evoked Aggression DOI
Harris S. Kaplan, Patricia M. Horvath, Mohammed Mostafizur Rahman

et al.

Physiological Reviews, Journal Year: 2024, Volume and Issue: 105(1), P. 315 - 381

Published: Aug. 15, 2024

Parenting behavior comprises a variety of adult-infant and adult-adult interactions across multiple timescales. The state transition from nonparent to parent requires an extensive reorganization individual priorities physiology is facilitated by combinatorial hormone action on specific cell types that are integrated throughout interconnected brainwide neuronal circuits. In this review, we take comprehensive approach integrate historical current literature each these topics species, with focus rodents. New emerging molecular, circuit-based, computational technologies have recently been used address outstanding gaps in our framework knowledge infant-directed behavior. This work raising fundamental questions about the interplay between instinctive learned components parenting mutual regulation affiliative versus agonistic behaviors health disease. Whenever possible, point how helped gain novel insights opened new avenues research into neurobiology parenting. We hope review will serve as introduction for those field, resource already studying parenting, guidepost designing future studies.

Language: Английский

Citations

1
California mice (Peromyscus californicus) adjust mouth movements for vocal production during early postnatal development DOI Creative Commons
Kristine L. Preston, Tobias Riede

BMC Biology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 24, 2024

The order Rodentia is the largest group of mammals. Diversification vocal communication has contributed to rodent radiation and allowed them occupy diverse habitats adopt different social systems. mechanism by which efficient sounds, carry over surprisingly large distances, are generated incompletely understood. Here we focused on development function rhythmic mouth movements laryngeal sound production. We studied spontaneously vocalizing California mice (Peromyscus californicus) through video recordings. Mouth gape was estimated from images characteristics were measured in synchronized coordinated their with production but differently two call types. In high-frequency whistles ("USV syllables"), present postnatal day 1 reduced within first 2 weeks life. prominently during sustained vocalizations ("SV became more adjusted syllable beginning end. Maximum correlated intensity fundamental frequency SV syllables. effect strongest most predictable when closed temporarily immobilizing mandible an elevated position. This study demonstrates that orofacial behavior not only plays a critical role determining acoustic features also shows remarkable adjustments early development.

Language: Английский

Citations

0
Acoustic communication: Deer mice join the chorus DOI Creative Commons
Alison J. Barker

Current Biology, Journal Year: 2023, Volume and Issue: 33(7), P. R264 - R266

Published: April 1, 2023

Language: Английский

Citations

1
Scientific and ethical challenges of brain chimeras converge on an ‘enriched’ approach DOI
J. Lomax Boyd

Molecular Psychology Brain Behavior and Society, Journal Year: 2023, Volume and Issue: 2, P. 16 - 16

Published: July 13, 2023

Transplantation of human brain organoids into nonhuman animals has the potential to advance our molecular understanding cognition. Yet hybridized nervous systems raise novel scientific and ethical questions that appear be oriented toward opposing ends – as models become more sophisticated, concerns salient. I posit challenges arising from interspecies engraftment may akin ‘birds a feather’ are entangled, rather than in tension. The value engrafted depends on extent which they provide insights neurobiology, while tend focus wellbeing animals. Based synthesis empirical theoretical evidence neuroscience philosophical bioethics, respectively, argue converge shared interest achieving fullest possible maturation host animal. conditions favorable also consistent with factors contribute elaboration neuronal features during development later behavioral traits. Treating if already possess higher moral status may, fact, necessary for them develop neuronal, cognitive, traits most relevant brain. Studies have shown enriched environments neurons mature, fully, neural circuits complex behaviors, supporting individuation conceptualize an ‘enriched’ approach research using offer case study examining how can used neurobiology speech grafted uncertainties posed by illustrates joint consideration perspectives reveal convergent pathways advancing research.

Language: Английский

Citations

1
Comparative Analysis of Tickling and Conspecific Play in Tame Mice and Golden Hamsters DOI Creative Commons

Sarah Dagher,

Darcie DeAngelo, Ren Y. Sato

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

Abstract Social play behavior is a fundamental aspect of animal interaction, shaping social bonds and enhancing cognitive capacity. While studies on human-animal interactions have primarily focused few selected species, research rodents beyond rats remains scarce. We, therefore, addressed the dynamics in tame mice, selectively bred to approach human hands, golden hamsters, comparing their responses during with humans conspecifics. Tame mice exhibited heightened playfulness humans, marked by increased vocalizations chasing behavior, addition conspecifics compared unselected control mice. Hamsters demonstrated stronger inclination towards conspecific interactions. Notably, vocalization patterns varied between heterospecific engagement both suggesting context-dependent communication. These findings offer insights into evolutionary basis influencing across species differing structures. Understanding these mechanisms enriches our comprehension diverse pathways through which animals form bonds.

Language: Английский

Citations

0
Ultrasonic vocalization emission is altered following neonatal hypoxic-ischemic brain injury in mice DOI Creative Commons

Eva C. Hermans,

Caroline G.M. de Theije, Cora H. Nijboer

et al.

Behavioural Brain Research, Journal Year: 2024, Volume and Issue: 471, P. 115113 - 115113

Published: June 14, 2024

Neonatal hypoxic-ischemic (HI) brain injury leads to cognitive impairments including social communication disabilities. Current treatments do not sufficiently target these impairments, therefore new tools are needed examine in models for neonatal injury. Ultrasonic vocalizations (USVs) during early life show potential as a measurement development and reflect landmark developmental stages mice. However, changes USV emission after HI have been found yet. Our current study examines patterns classes the first 3 days C57Bl/6 mice were subjected on postnatal day (P)9 USVs recorded between P10 P12. Audio files analyzed using VocalMat automated tool. HI-injured emitted less USVs, shorter durations, at higher frequency compared control (sham-operated) littermates. The HI-induced alterations most distinct range of 50-75 kHz. At mouse pups also produced different ratios class types Moreover, duration specific certain animals controls. Injury striatum hippocampus contributed HI. Overall, newborn which could be used tool HI-related deficits.

Language: Английский

Citations

0