A promoter polymorphism defines distinct roles in anther development for Col‐0 and Ler‐0 alleles of Arabidopsis ACYL‐COA BINDING PROTEIN3 DOI Creative Commons
Ze‐Hua Guo, Tai‐Hua Hu, Mohd Fadhli Hamdan

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 243(4), P. 1424 - 1439

Published: June 24, 2024

Summary Acyl‐CoA‐Binding Proteins (ACBPs) bind acyl‐CoA esters and function in lipid metabolism. Although acbp3‐1 , the ACBP3 mutant Arabidopsis thaliana ecotype Col‐0, displays normal floral development, acbp3‐2 from L er ‐0 characterized herein exhibits defective adaxial anther lobes improper sporocyte formation. To understand these differences identify role of ERECTA function, acbp3 mutants acbp3‐erecta ( ) lines were analyzed by microscopy for morphology high‐performance liquid chromatography composition. Defects Landsberg development related to ERECTA‐mediated pathway because progenies × La‐0 er‐1 Col‐0 showed anthers, contrasting that ‐0. Polymorphism regulatory region enabled its but not which harbored an AT‐repeat insertion. expression restored using ACBP3pro (Ler)::ACBP3 (Col)::ACBP3. SPOROCYTELESS (SPL), a formation regulator activated transcription Col‐0. For ERECTA‐related is required ‐0, The disrupted promoter SPL binding eliminates development.

Language: Английский

Flowering time: From physiology, through genetics to mechanism DOI Creative Commons
Robert Maple, Pan Zhu, Jo Hepworth

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(1), P. 190 - 212

Published: Feb. 28, 2024

Abstract Plant species have evolved different requirements for environmental/endogenous cues to induce flowering. Originally, these varying were thought reflect the action of molecular mechanisms. Thinking changed when genetic and analysis in Arabidopsis thaliana revealed that a network environmental endogenous signaling input pathways converge regulate common set “floral pathway integrators.” Variation predominance within can generate diversity observed species. Many genes identified by flowering time mutants found encode general developmental gene regulators, with their targets having specific function. Studies natural variation more successful at identifying acting as nodes central adaptation domestication. Attention has now turned mechanistic dissection function how during adaptation. This will inform breeding strategies climate-proof crops help define which act critical many other

Language: Английский

Citations

27
COOLAIR and PRC2 function in parallel to silence FLC during vernalization DOI Creative Commons
Mathias Nielsen, Govind Menon, Yusheng Zhao

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(4)

Published: Jan. 18, 2024

Noncoding transcription induces chromatin changes that can mediate environmental responsiveness, but the causes and consequences of these mechanisms are still unclear. Here, we investigate how antisense (termed COOLAIR ) interfaces with Polycomb Repressive Complex 2 (PRC2) silencing during winter-induced epigenetic regulation Arabidopsis FLOWERING LOCUS C ( FLC ). We use genetic analyses on lines ineffective or hyperactive for pathway in combination computational modeling to define underlying repression. Our results show is silenced through pathways function different dynamics: a transcription-mediated capable fast response parallel slow PRC2 switching mechanism maintains each allele an epigenetically state. Components both regulated by common transcriptional regulator (NTL8), which accumulates reduced dilution due growth at low temperature. The activities regulatory steps, their control temperature-dependent dynamics, create flexible system registering widely fluctuating natural temperature conditions change year year, yet ensure robust FLC.

Language: Английский

Citations

10
Retrotransposon-driven environmental regulation of FLC leads to adaptive response to herbicide DOI

Mathieu Raingeval,

Basile Leduque,

Pierre Baduel

et al.

Nature Plants, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 27, 2024

Language: Английский

Citations

7
GhGASA14 regulates the flowering time of upland cotton in response to GA3 DOI
Ying Li,

Wenmin Yuan,

Jialuo Peng

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(7)

Published: June 13, 2024

Language: Английский

Citations

4
Correlation between SNPs in promoter region of gene, L-Type lectin (LvLTLC1) and the resistance trait of shrimp, Litopenaeus vannamei, against vibrios DOI

Shuyang Wen,

Lihong Yuan,

Jiayue Yin

et al.

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 41, P. 102725 - 102725

Published: March 1, 2025

Language: Английский

Citations

0
GhGASA14 regulates the flowering time of upland cotton in response to GA3 DOI Creative Commons
Ying Li,

W. Yuan,

Jialuo Peng

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 8, 2024

Abstract The gibberellic acid-stimulated Arabidopsis (GASA), a gibberellin-regulated short amino acid family, has been extensively investigated in several plant species and found to be critical for growth development. However, limited research reported cotton. In this study, we identified 38 GhGASAs that were dispersed across 18 chromosomes upland cotton, all of these genes had GASA core domain. Transcriptome expression patterns qRT‒PCR results revealed GhGASA9 GhGASA14 exhibited upregulated not only the floral organs but also leaves early-maturity cultivars. two further functionally characterized by virus-induced gene silencing (VIGS) budding flowering times after target later than those control (TRV:00). Exogenous application GA3 made period different fruiting branches more concentrated compared with water-treated group (MOCK). Interestingly, allelic variation was detected coding sequence GhGASA14 between early‐maturing late‐maturing accessions, frequency favorable allele greater high-latitude cotton varieties low-latitude ones. Additionally, significant linear relationship observed between level time among 12 accessions. Taken together, indicated may positively regulate respond GA3. These findings could lead use valuable genetic resources breeding future.

Language: Английский

Citations

0
Cryptic Variation in Adaptive Phenotypes Revealed by PanspecificflcMutants DOI Creative Commons
Ulrich Lutz, Ilja Bezrukov, Rebecca Schwab

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 17, 2024

The study of mutants is one the best tools for understanding genetic basis phenotypes that contribute to adaptation. Oddly, mutant analyses are almost always restricted single backgrounds and findings therefore can not be easily generalized. A case in point key regulator flowering, FLOWERING LOCUS C ( FLC ), which has been inferred explain much flowering time variation Arabidopsis thaliana , yet have examined very few backgrounds. We previously established a set panspecific flc 62 accessions A. (Ruffley et al. 2024). Here, we investigate how background modulates effects on vegetative traits, as well physiology transcriptomes. Time onset genome-edited lines was reduced by up 83%, but considerable remained. Genetic mapping showed extremely early absence mostly explained natural at known target FT with additional contribution from loci colocalizing . Prognostic sequence did suggest extremely-early combinations engineered alleles would deleterious, represented commonly used collections accessions. To test whether this discrepancy could due sampling bias, undertook focused collection effort wild populations Southern Italy, confirmed exist populations. Apart its specific role regulation, pleiotropic other ecophysiological traits such growth, these were also dependent background, further supported transcriptomic comparisons. Together conclude various roles greatly diversified different Our provides proof-of-concept analysis reveal true extent networks focal gene participates in.

Language: Английский

Citations

0
AtSNP_TATAdb: Candidate Molecular Markers of Plant Advantages Related to Single Nucleotide Polymorphisms within Proximal Promoters of Arabidopsis thaliana L. DOI Open Access
Anton Bogomolov,

Karina Zolotareva,

Sergey Filonov

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(1), P. 607 - 607

Published: Jan. 3, 2024

The mainstream of the post-genome target-assisted breeding in crop plant species includes biofortification such as high-throughput phenotyping along with genome-based selection. Therefore, this work, we used Web-service Plant_SNP_TATA_Z-tester, which have previously developed, to run a uniform silico analysis transcriptional alterations 54,013 protein-coding transcripts from 32,833 Arabidopsis thaliana L. genes caused by 871,707 SNPs located proximal promoter region. identified 54,993 significantly decreasing or increasing gene expression through changes TATA-binding protein affinity promoters. existence these highly conserved promoters may be explained intraspecific diversity kept stabilizing natural To support this, hand-annotated papers on some possessing their orthologs other and demonstrated effects expressions vital traits. We integrated estimates TBP-promoter AtSNP_TATAdb knowledge base showed significant correlations independent vivo experimental data. These appeared robust variations statistical criteria, genomic environment TATA box regions, plants growing conditions.

Language: Английский

Citations

0
A promoter polymorphism defines distinct roles in anther development for Col‐0 and Ler‐0 alleles of Arabidopsis ACYL‐COA BINDING PROTEIN3 DOI Creative Commons
Ze‐Hua Guo, Tai‐Hua Hu, Mohd Fadhli Hamdan

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 243(4), P. 1424 - 1439

Published: June 24, 2024

Summary Acyl‐CoA‐Binding Proteins (ACBPs) bind acyl‐CoA esters and function in lipid metabolism. Although acbp3‐1 , the ACBP3 mutant Arabidopsis thaliana ecotype Col‐0, displays normal floral development, acbp3‐2 from L er ‐0 characterized herein exhibits defective adaxial anther lobes improper sporocyte formation. To understand these differences identify role of ERECTA function, acbp3 mutants acbp3‐erecta ( ) lines were analyzed by microscopy for morphology high‐performance liquid chromatography composition. Defects Landsberg development related to ERECTA‐mediated pathway because progenies × La‐0 er‐1 Col‐0 showed anthers, contrasting that ‐0. Polymorphism regulatory region enabled its but not which harbored an AT‐repeat insertion. expression restored using ACBP3pro (Ler)::ACBP3 (Col)::ACBP3. SPOROCYTELESS (SPL), a formation regulator activated transcription Col‐0. For ERECTA‐related is required ‐0, The disrupted promoter SPL binding eliminates development.

Language: Английский

Citations

0