A pathogen effector co-opts a host RabGAP protein to remodel pathogen interface and subvert defense-related secretion DOI Creative Commons
Enoch Lok Him Yuen, Yasin Tümtaş, Freddie J. King

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(40)

Published: Oct. 4, 2024

Pathogens have evolved sophisticated mechanisms to manipulate host cell membrane dynamics, a crucial adaptation survive in hostile environments shaped by innate immune responses. Plant-derived interfaces, engulfing invasive hyphal projections of fungal and oomycete pathogens, are prominent junctures dictating infection outcomes. Understanding how pathogens transform these host-pathogen interfaces their advantage remains key biological question. Here, we identified conserved effector, secreted plant pathogenic oomycetes, that co-opts Rab GTPase-activating protein (RabGAP), TOPGAP, remodel the interface. The PiE354, hijacks TOPGAP as susceptibility factor usurp its GAP activity on Rab8a, GTPase for defense-related secretion. By hijacking PiE354 purges Rab8a from plasma membrane, diverting Rab8a-mediated trafficking away pathogen This mechanism signifies an uncanny evolutionary effector co-opting regulatory component subvert secretion, thereby providing unprecedented mechanistic insights into reprogramming dynamics pathogens.

Language: Английский

Paired C-type lectin receptors mediate specific recognition of divergent oomycete pathogens in C. elegans DOI Creative Commons
Kenneth Liu, Manish Grover, Franziska Trusch

et al.

Cell Reports, Journal Year: 2024, Volume and Issue: 43(11), P. 114906 - 114906

Published: Oct. 25, 2024

Language: Английский

Citations

2
A PRA-Rab trafficking machinery modulates NLR immune receptor plasma membrane microdomain anchoring and blast resistance in rice DOI Creative Commons
Di Liang, Dongyong Yang, Tai Li

et al.

Science Bulletin, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

2
Paired C-type lectin receptors mediate specific recognition of divergent oomycete pathogens in C. elegans. DOI Creative Commons
Kenneth Liu, Manish Grover, Franziska Trusch

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 8, 2024

Summary Innate immune responses can be initiated through the detection of pathogen or damage-associated molecular patterns by host receptors that are often present on surface cells. While certain invertebrates like Caenorhabditis elegans lack professional cells, they still respond to infection in a pathogen-specific manner. It has been debated for years whether homologues canonical recognition also functioning nematode. Here we show C-type lectin mediate species-specific divergent oomycetes C. elegans. A CLEC-27/CLEC-35 pair is essential oomycete Myzocytiopsis humicola , while CLEC-26/CLEC-36 required Haptoglossa zoospora. Both clec pairs transcriptionally regulated shared promoter conserved PRD-like homeodomain transcription factor CEH-37/OTX2 and act sensory neurons anterior intestine trigger protective response epidermis. This system enables redundant tissue sensing threats CLEC defense via cross-tissue communication. Highlights zoospora co-regulated function redundantly

Language: Английский

Citations

0
Linking secretion and cytoskeleton in immunity– a case for Arabidopsis TGNap1 DOI Creative Commons
Deepak D. Bhandari,

Federica Brandizzi

BioEssays, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 20, 2024

Abstract In plants, robust defense depends on the efficient and resilient trafficking supply chains to site of pathogen attack. Though importance intracellular in plant immunity has been well established, a lack clarity remains regarding contribution various pathways transporting immune‐related proteins. We have recently identified trans‐Golgi network protein, TGN‐ASSOCIATED PROTEIN 1 (TGNap1), which functionally links post‐Golgi vesicles with cytoskeleton transport immunity‐related proteins model species Arabidopsis thaliana . propose new hypotheses functional implications TGNap1 then elaborate surprising heterogeneity TGN during revealed by discovery other TGN‐associated recent years.

Language: Английский

Citations

0
A pathogen effector co-opts a host RabGAP protein to remodel pathogen interface and subvert defense-related secretion DOI Creative Commons
Enoch Lok Him Yuen, Yasin Tümtaş, Freddie J. King

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(40)

Published: Oct. 4, 2024

Pathogens have evolved sophisticated mechanisms to manipulate host cell membrane dynamics, a crucial adaptation survive in hostile environments shaped by innate immune responses. Plant-derived interfaces, engulfing invasive hyphal projections of fungal and oomycete pathogens, are prominent junctures dictating infection outcomes. Understanding how pathogens transform these host-pathogen interfaces their advantage remains key biological question. Here, we identified conserved effector, secreted plant pathogenic oomycetes, that co-opts Rab GTPase-activating protein (RabGAP), TOPGAP, remodel the interface. The PiE354, hijacks TOPGAP as susceptibility factor usurp its GAP activity on Rab8a, GTPase for defense-related secretion. By hijacking PiE354 purges Rab8a from plasma membrane, diverting Rab8a-mediated trafficking away pathogen This mechanism signifies an uncanny evolutionary effector co-opting regulatory component subvert secretion, thereby providing unprecedented mechanistic insights into reprogramming dynamics pathogens.

Language: Английский

Citations

0