A frameshift mutation in JAZ10 resolves the growth versus defense dilemma in rice DOI Creative Commons
Lei‐Lei Li,

Yujie Xiao,

Baohui Wang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(52)

Published: Dec. 18, 2024

CRISPR-Cas9 genome editing systems have revolutionized plant gene functional studies by enabling the targeted introduction of insertion-deletions (INDELs) via nonhomologous end-joining (NHEJ) pathway. Frameshift-inducing INDELs can introduce a premature termination codon and, in other instances, lead to appearance new proteins. Here, we found that mutations rice jasmonate (JA) signaling OsJAZ10 CRISPR-Cas9-based did not affect canonical JA signaling. However, type mutant with an INDEL yielded novel frameshift protein named FJ10 ( F rameshift mutation J AZ 10 ), exhibited enhanced growth and increased resistance brown planthopper attacks. Overexpression wild-type plants phenocopies mutants. Further characterization revealed interacts Slender Rice 1 (OsSLR1) F-box/Kelch 16 (OsFBK16). These interactions disrupt function OsSLR1 suppressing gibberellin-mediated OsFBK16 repressing lignin-mediated defense responses, respectively. Field experiments -expressing demonstrate this uncouples growth–defense tradeoff, opening broad avenues obtain cultivars yield without compromised defenses.

Language: Английский

Adaptability Analysis of Tuta absoluta to Different Hosts and Related Salivary Genes Identification DOI

Xiujie Li,

Xiang-Yun Cai, Luo-Hua Shang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Tuta absoluta is a significant agricultural pest primarily affecting Solanaceae plants, resulting in substantial economic losses agriculture. Insect saliva an intermediary between insects and playing crucial role modulating host adaptability plant defense. This study analyzed the adaptive differences of T. on four plants using two-sex life table method. Results indicated that to tobacco worse than its other three varieties tomatoes. The salivary gland transcriptome analysis signal peptide prediction revealed Trypsin, B5 V51-1498, Ta74 were highly expressed glands subjected treatment exhibit characteristics secretory proteins, alongside feeding selection differences. Our findings elucidate strategies larvae various offer new insights into protein-mediated defense processes.

Language: Английский

Citations

0
Insight into Rice Resistance to the Brown Planthopper: Gene Cloning, Functional Analysis, and Breeding Applications DOI Open Access
Yangdong Ye,

Shangye Xiong,

Xin Guan

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(24), P. 13397 - 13397

Published: Dec. 13, 2024

This review provides a comprehensive overview of the current understanding rice resistance to brown planthopper (BPH), major pest that poses significant threats production through direct feeding damage and by transmitting viruses such as Rice grassy stunt virus (RGSV) ragged (RRSV). We highlight emergence various BPH biotypes have overcome specific genes in rice. Advances genetic mapping cloning identified 17 genes, classified into typical R encoding nucleotide-binding leucine-rich repeat (NLR) proteins atypical lectin receptor kinases affecting cell wall composition. The molecular mechanisms these involve activation plant defense pathways mediated phytohormones like jasmonic acid (JA), salicylic (SA), ethylene, well defensive metabolites. also examine complex interactions between salivary responses, noting how effectors can both suppress trigger immunity. development improvement BPH-resistant varieties conventional breeding marker-assisted selection are discussed, including strategies gene pyramiding enhance durability. Finally, we outline challenges future directions for durable resistance, emphasizing need continued research on with broad-spectrum long-lasting resistance.

Language: Английский

Citations

1
Impact of arbuscular mycorrhizal fungi on maize rhizosphere microbiome stability under moderate drought conditions DOI
Yalin Chen,

Chunyu Sun,

Yuxin Yan

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 290, P. 127957 - 127957

Published: Oct. 30, 2024

Language: Английский

Citations

1
Recent Advances in Gene Mining and Hormonal Mechanism for Brown Planthopper Resistance in Rice DOI Open Access
Xiao Zhang,

Dongfang Gu,

Daoming Liu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12965 - 12965

Published: Dec. 2, 2024

Rice (

Language: Английский

Citations

1
A frameshift mutation in JAZ10 resolves the growth versus defense dilemma in rice DOI Creative Commons
Lei‐Lei Li,

Yujie Xiao,

Baohui Wang

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(52)

Published: Dec. 18, 2024

CRISPR-Cas9 genome editing systems have revolutionized plant gene functional studies by enabling the targeted introduction of insertion-deletions (INDELs) via nonhomologous end-joining (NHEJ) pathway. Frameshift-inducing INDELs can introduce a premature termination codon and, in other instances, lead to appearance new proteins. Here, we found that mutations rice jasmonate (JA) signaling OsJAZ10 CRISPR-Cas9-based did not affect canonical JA signaling. However, type mutant with an INDEL yielded novel frameshift protein named FJ10 ( F rameshift mutation J AZ 10 ), exhibited enhanced growth and increased resistance brown planthopper attacks. Overexpression wild-type plants phenocopies mutants. Further characterization revealed interacts Slender Rice 1 (OsSLR1) F-box/Kelch 16 (OsFBK16). These interactions disrupt function OsSLR1 suppressing gibberellin-mediated OsFBK16 repressing lignin-mediated defense responses, respectively. Field experiments -expressing demonstrate this uncouples growth–defense tradeoff, opening broad avenues obtain cultivars yield without compromised defenses.

Language: Английский

Citations

1