Giant polyketide synthase enzymes in the biosynthesis of giant marine polyether toxins

Science, Journal Year: 2024, Volume and Issue: 385(6709), P. 671 - 678

Published: Aug. 8, 2024

Prymnesium parvum are harmful haptophyte algae that cause massive environmental fish kills. Their polyketide polyether toxins, the prymnesins, among largest nonpolymeric compounds in nature and have biosynthetic origins remained enigmatic for more than 40 years. In this work, we report “PKZILLAs,” P. synthase (PKS) genes evaded previous detection. PKZILLA-1 -2 encode giant protein products of 4.7 3.2 megadaltons 140 99 enzyme domains. predicted polyene product matches proposed pre-prymnesin precursor 90-carbon–backbone A-type prymnesins. We further characterize variant PKZILLA-B1, which is responsible shorter B-type analog prymnesin-B1, from RCC3426 thus establish a general model logic. This work expands expectations genetic enzymatic size limits biology.

Language: Английский

Genetic, metabolic and toxicological diversity within Prymnesium parvum (Haptophyte) from Polish waterbodies

Water Research, Journal Year: 2025, Volume and Issue: 282, P. 123744 - 123744

Published: April 30, 2025

Language: Английский