Exploration of the Differential Expression Patterns of Immunoglobulin Heavy Chain Genes in Horses and Donkeys

Developmental & Comparative Immunology, Journal Year: 2025, Volume and Issue: unknown, P. 105360 - 105360

Published: March 1, 2025

Language: Английский

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Molecular characterization and complexity of the immunoglobulin repertoire in the silver-black fox (Vulpes vulpes)

BMC Veterinary Research, Journal Year: 2025, Volume and Issue: 21(1)

Published: March 28, 2025

Immunoglobulins, a class of globulins with antibody properties, play crucial role in the body's defense against pathogens. In this study, we analyzed gene loci structure Silver-black fox using comparative genomics approach. The mechanisms expression diversity and its preferences were investigated through Next-generation sequencing (NGS). results revealed 32 potentially functional VH genes, 9 Vκ 17 Vλ DH 3 JH 6 Jκ 11 Jλ located on different scaffolds. Subsequently, 5'RACE PE300 bipartite used to obtain reads expressed repertoire foxes rearrangement events. analysis indicated strong preference use V genes J by fox. main ways V(D)J recombination somatic hypermutation (SHM). hypermutated region SHM was not only concentrated CDR but also had higher mutation rate FR region. types G > A, C T, T C, A G. findings study could serve as theoretical foundation for deeper understanding immunoglobulins, which is significant enriching knowledge immunogenetics providing support future studies vaccine design

Language: Английский

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Immunoglobulin gene loci structure and diversity of raccoon dog (Nyctereutes procyonoides)

BMC Genomics, Journal Year: 2025, Volume and Issue: 26(1)

Published: April 29, 2025

In this study, we analyzed the loci structure of raccoon dog genes using a comparative genomics approach. We investigated mechanism expression diversity and its preference Next-generation sequencing. The results indicated that gene were similar to most mammals, with relative abundance V(D)J genes, which ensures diversity. identified following potentially functional genes: 7 VH 6 Vκ 23 Vλ 24 DH 5 JH 4 Jκ Jλ arranged in manner other mammals. Analysis showed have strong for use V same was true genes. main modes recombination somatic hypermutation, high mutation region hypermutation not only concentrated CDR region, but also has FR types G > A, C T, T C, A G. Interestingly, compared differences between normal diseased dogs, there significant (P < 0.05), whereas no expression, utilization preference, recombination, types. summary, described IgH IgL dog, mapped loci, PE300 study could provide theoretical basis an in-depth understanding immunoglobulins, references disease-resistant breeding.

Language: Английский

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Structural profile and diversity of immunoglobulin genes in the Arctic Fox

The Veterinary Journal, Journal Year: 2024, Volume and Issue: unknown, P. 106295 - 106295

Published: Dec. 1, 2024

Language: Английский

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