Neural circuitry linking mating and egg laying in Drosophila females

Nature, Journal Year: 2020, Volume and Issue: 579(7797), P. 101 - 105

Published: Feb. 26, 2020

Language: Английский

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Nested neural circuits generate distinct acoustic signals during Drosophila courtship

Current Biology, Journal Year: 2024, Volume and Issue: 34(4), P. 808 - 824.e6

Published: Jan. 30, 2024

Many motor control systems generate multiple movements using a common set of muscles. How are premotor circuits able to flexibly diverse movement patterns? Here, we characterize the neuronal that drive distinct courtship songs Drosophila melanogaster. Male flies vibrate their wings toward females produce two different song modes—pulse and sine song—which signal species identity male quality. Using cell-type-specific genetic reagents connectome, provide cellular synaptic map in ventral nerve cord these examine how activating or inhibiting each cell type within affects song. Our data reveal circuit is organized into nested feedforward pathways with extensive reciprocal feedback connections. The larger network produces pulse song, more complex ancestral form. A subset this simpler recent Such organization may be feature which evolution has layered increasing flexibility onto basic pattern.

Language: Английский

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Changes in the cellular makeup of motor patterning circuits drive courtship song evolution in Drosophila

Current Biology, Journal Year: 2024, Volume and Issue: 34(11), P. 2319 - 2329.e6

Published: April 29, 2024

Language: Английский

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Functional Imaging and Optogenetics in Drosophila

Genetics, Journal Year: 2018, Volume and Issue: 208(4), P. 1291 - 1309

Published: April 1, 2018

Abstract Understanding how activity patterns in specific neural circuits coordinate an animal’s behavior remains a key area of neuroscience research. Genetic tools and brain tractable complexity make Drosophila premier model organism for these studies. Here, we review the wealth reagents available to map manipulate neuronal with light.

Language: Английский

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Genetic and neuronal mechanisms governing the sex-specific interaction between sleep and sexual behaviors in Drosophila

Nature Communications, Journal Year: 2017, Volume and Issue: 8(1)

Published: July 21, 2017

Animals execute one particular behavior among many others in a context-dependent manner, yet the mechanisms underlying such behavioral choice remain poorly understood. Here we studied how two fundamental behaviors, sex and sleep, interact at genetic neuronal levels Drosophila. We show that an increased need for sleep inhibits male sexual by decreasing activity of male-specific P1 neurons coexpress determination genes fru M dsx, but does not affect female behavior. Further, delineate sex-specific circuit wherein encoding courtship drive suppressed forming mutually excitatory connections with -positive sleep-controlling DN1 neurons. In addition, find FRUM regulates through distinct neural substrates. These studies reveal basis interaction between behaviors Drosophila, provide insights into competing are co-regulated.Genes circuits involved arousal have been extensively authors identify fruitless doublesex, P1-DN1 feedback governs these behaviors.

Language: Английский

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Classification and genetic targeting of cell types in the primary taste and premotor center of the adult Drosophila brain

eLife, Journal Year: 2021, Volume and Issue: 10

Published: Sept. 2, 2021

Neural circuits carry out complex computations that allow animals to evaluate food, select mates, move toward attractive stimuli, and away from threats. In insects, the subesophageal zone (SEZ) is a brain region receives gustatory, pheromonal, mechanosensory inputs contributes control of diverse behaviors, including feeding, grooming, locomotion. Despite its importance in sensorimotor transformations, study SEZ has been hindered by limited knowledge underlying diversity neurons. Here, we generate collection split-GAL4 lines provides precise genetic targeting 138 different cell types adult Drosophila melanogaster, comprising approximately one third all We characterize single-cell anatomy these neurons find they cluster morphology into six supergroups organize discrete anatomical domains. majority local interneurons are not classically polarized, suggesting rich processing, whereas projection tend be conveying information number higher regions. This insight organization generates resources will facilitate further their contributions sensory processing behavior.

Language: Английский

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Descending neurons coordinate anterior grooming behavior in Drosophila

Current Biology, Journal Year: 2022, Volume and Issue: 32(4), P. 823 - 833.e4

Published: Feb. 1, 2022

The brain coordinates the movements that constitute behavior, but how descending neurons convey myriad of commands required to activate motor limbs in right order and combinations produce those is not well understood. For anterior grooming behavior fly, we show its component head sweeps leg rubs can be initiated separately, or as a set, by different neurons. Head are mutually exclusive front legs normally alternate, circuits ventral nerve cord resolve competing commands. Finally, left must work together remove debris. coordination for achieved unilateral activation single neuron, while similar manipulation neuron decouples single-sided sweeps. Taken together, these results demonstrate distinct orchestrate complex alternation between make up grooming.

Language: Английский

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Systematic annotation of a complete adult maleDrosophilanerve cord connectome reveals principles of functional organisation

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 6, 2023

Summary Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ system hierarchical coarse annotations group similar across midline segments, then define systematic cell types sensory neurons, intrinsic ascending non-motor efferent neurons. Stereotyped arrays neuroblasts generate related neuron populations called hemilineages repeat segments cord. confirm larval-born from given hemilineage generally express same neurotransmitter but find earlier born often different one. match over 35% intrinsic, ascending, defining serial sets which were crucial typing motor assign modality 5000 cluster by connectivity, identify serially homologous layered organisation likely corresponding peripheral topography. Finally, present selected examples circuits predicated on programmatic analysis VNC connectome. Our are critical analysing structure descending input output, both described in third (Cheong 2023). These being released part neuprint.janelia.org clio.janelia.org web applications also serve basis connectome through dedicated tools paper.

Language: Английский

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Single-cell type analysis of wing premotor circuits in the ventral nerve cord ofDrosophila melanogaster

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 1, 2023

To perform most behaviors, animals must send commands from higher-order processing centers in the brain to premotor circuits that reside ganglia distinct brain, such as mammalian spinal cord or insect ventral nerve cord. How these are functionally organized generate great diversity of animal behavior remains unclear. An important first step unraveling organization is identify their constituent cell types and create tools monitor manipulate with high specificity assess function. This possible tractable fly. a toolkit, we used combinatorial genetic technique (split-GAL4) 195 sparse driver lines targeting 198 individual These included wing haltere motoneurons, modulatory neurons, interneurons. Using combination behavioral, developmental, anatomical analyses, systematically characterized targeted our collection. Taken together, resources results presented here form powerful toolkit for future investigations neural connectivity while linking them behavioral outputs.

Language: Английский

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Systematic annotation of a complete adult male Drosophila nerve cord connectome reveals principles of functional organisation

Published: July 22, 2024

Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ system hierarchical coarse annotations group similar across midline segments, then define systematic cell types sensory neurons, intrinsic ascending non-motor efferent neurons. Stereotyped arrays neuroblasts generate related neuron populations called hemilineages repeat segments cord. confirm larval-born from given hemilineage generally express same neurotransmitter but find earlier born often different one. match over 35% intrinsic, ascending, defining serial sets which were crucial typing motor assign modality 5000 cluster by connectivity, identify serially homologous layered organisation likely corresponding peripheral topography. Finally, present selected examples circuits predicated on programmatic analysis VNC connectome. Our are critical analysing structure descending input output, both described in third (Cheong 2023). These being released part neuprint.janelia.org clio.janelia.org web applications also serve basis connectome through dedicated tools paper.

Language: Английский

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