A leaky integrate-and-fire computational model based on the connectome of the entire adultDrosophilabrain reveals insights into sensorimotor processing DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Nico Spiller

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 2, 2023

Abstract The forthcoming assembly of the adult Drosophila melanogaster central brain connectome, containing over 125,000 neurons and 50 million synaptic connections, provides a template for examining sensory processing throughout brain. Here, we create leaky integrate-and-fire computational model entire brain, based on neural connectivity neurotransmitter identity, to study circuit properties feeding grooming behaviors. We show that activation sugar-sensing or water-sensing gustatory in accurately predicts respond tastes are required initiation. Computational region those elicit motor neuron firing, testable hypothesis validate by optogenetic behavioral studies. Moreover, different classes makes accurate predictions how multiple taste modalities interact, providing circuit-level insight into aversive appetitive processing. Our sugar water pathways form partially shared initiation pathway, which our calcium imaging experiments confirm. Additionally, applied this mechanosensory circuits found small set comprising antennal do not overlap with circuits, describes response upon subtypes. results demonstrate modeling purely from predicted identity generates experimentally hypotheses can describe complete sensorimotor transformations.

Language: Английский

Neuronal wiring diagram of an adult brain DOI Creative Commons
Sven Dorkenwald, Arie Matsliah, Amy Sterling

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 124 - 138

Published: Oct. 2, 2024

Language: Английский

Citations

139
Neuronal wiring diagram of an adult brain DOI Creative Commons
Sven Dorkenwald, Arie Matsliah, Amy Sterling

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 30, 2023

Abstract Connections between neurons can be mapped by acquiring and analyzing electron microscopic (EM) brain images. In recent years, this approach has been applied to chunks of brains reconstruct local connectivity maps that are highly informative, yet inadequate for understanding function more globally. Here, we present the first neuronal wiring diagram a whole adult brain, containing 5×10 7 chemical synapses ∼130,000 reconstructed from female Drosophila melanogaster . The resource also incorporates annotations cell classes types, nerves, hemilineages, predictions neurotransmitter identities. Data products available download, programmatic access, interactive browsing made interoperable with other fly data resources. We show how derive projectome, map projections regions, connectome. demonstrate tracing synaptic pathways analysis information flow inputs (sensory ascending neurons) outputs (motor, endocrine, descending neurons), across both hemispheres, central optic lobes. Tracing subset photoreceptors all way motor illustrates structure uncover putative circuit mechanisms underlying sensorimotor behaviors. technologies open ecosystem FlyWire Consortium set stage future large-scale connectome projects in species.

Language: Английский

Citations

134
A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution DOI Creative Commons
Geoffrey W. Meissner, Aljoscha Nern, Zachary Dorman

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Feb. 23, 2023

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of

Language: Английский

Citations

85
NeuronBridge: an intuitive web application for neuronal morphology search across large data sets DOI Creative Commons
Jody Clements, Cristian Goina, Philip M. Hubbard

et al.

BMC Bioinformatics, Journal Year: 2024, Volume and Issue: 25(1)

Published: March 15, 2024

Abstract Background Neuroscience research in Drosophila is benefiting from large-scale connectomics efforts using electron microscopy (EM) to reveal all the neurons a brain and their connections. To exploit this knowledge base, researchers relate connectome’s structure neuronal function, often by studying individual neuron cell types. Vast libraries of fly driver lines expressing fluorescent reporter genes sets have been created imaged confocal light (LM), enabling targeting for experimentation. However, creating line driving gene expression within single found an EM connectome remains challenge, as it typically requires identifying pair where only interest expressed both. This task other emerging scientific workflows require finding similar across large data different modalities. Results Here, we present NeuronBridge, web application easily rapidly putative morphological matches between We describe functionality construction NeuronBridge service, including its user-friendly graphical user interface (GUI), extensible model, serverless cloud architecture, massively parallel image search engine. Conclusions fills critical gap workflow used hundreds neuroscience around world. offer our software code, open APIs, processed integration reuse, provide service at http://neuronbridge.janelia.org .

Language: Английский

Citations

26
Transforming descending input into behavior: The organization of premotor circuits in the Drosophila Male Adult Nerve Cord connectome DOI Open Access
Han SJ Cheong, Katharina Eichler, Tomke Stürner

et al.

Published: March 18, 2024

In most animals, a relatively small number of descending neurons (DNs) connect higher brain centers in the animal’s head to circuits and motor (MNs) nerve cord body that effect movement limbs. To understand how signals generate behavior, it is critical these pathways are organized onto MNs. fly, Drosophila melanogaster , MNs controlling muscles leg, wing, other systems reside ventral (VNC), analogous mammalian spinal cord. companion papers, we introduced densely-reconstructed connectome Male Adult Nerve Cord (MANC, Takemura et al., 2023), including cell type developmental lineage annotation (Marin which provides complete VNC connectivity at synaptic resolution. Here, present first look organization networks connecting DNs based on this new information. We proofread curated all ensure accuracy reliability, then systematically matched DN axon terminals MN dendrites with light microscopy data link their morphology inputs or muscle targets. report both broad organizational patterns entire network fine-scale analysis selected interest. discover direct DN-MN connections infrequent identify communities intrinsic linked control different systems, putative for walking, dorsal flight steering power generation, intermediate lower tectulum coordinated action wings legs. Our generates hypotheses future functional experiments and, together MANC connectome, empowers others investigate richer mechanistic detail.

Language: Английский

Citations

24
Descending networks transform command signals into population motor control DOI Creative Commons
Jonas Braun, Femke Hurtak, Sibo Wang

et al.

Nature, Journal Year: 2024, Volume and Issue: 630(8017), P. 686 - 694

Published: June 5, 2024

Abstract To convert intentions into actions, movement instructions must pass from the brain to downstream motor circuits through descending neurons (DNs). These include small sets of command-like that are sufficient drive behaviours 1 —the circuit mechanisms for which remain unclear. Here we show DNs in Drosophila directly recruit networks additional orchestrate require active control numerous body parts. Specifically, found previously thought alone 2–4 fact co-activate larger populations DNs. Connectome analyses and experimental manipulations revealed this functional recruitment can be explained by direct excitatory connections between interconnected brain. Descending population is necessary behavioural control: with many partners network co-activation complete only simple stereotyped movements their absence. DN reside within behaviour-specific clusters inhibit one another. results support a mechanism generated increasingly large compose combining multiple subroutines.

Language: Английский

Citations

19
A Drosophila computational brain model reveals sensorimotor processing DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Nico Spiller

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 210 - 219

Published: Oct. 2, 2024

Language: Английский

Citations

19
Neural circuit mechanisms underlying context-specific halting in Drosophila DOI Creative Commons

Neha Sapkal,

Nino Mancini,

Divya Sthanu Kumar

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 191 - 200

Published: Oct. 2, 2024

Language: Английский

Citations

18
Serotonergic neurons translate taste detection into internal nutrient regulation DOI Creative Commons
Zepeng Yao, Kristin Scott

Neuron, Journal Year: 2022, Volume and Issue: 110(6), P. 1036 - 1050.e7

Published: Jan. 19, 2022

The nervous and endocrine systems coordinately monitor regulate nutrient availability to maintain energy homeostasis. Sensory detection of food regulates internal in a manner that anticipates intake, but sensory pathways promote anticipatory physiological changes remain unclear. Here, we identify serotonergic (5-HT) neurons as critical mediators transform gustatory by into the activation insulin-producing cells enteric Drosophila. One class 5-HT responds sugars, excites cells, limits consumption, suggesting they anticipate increased levels prevent overconsumption. A second bitter compounds activates gastric motility, likely stimulate digestion increase circulating nutrients upon rejection. These studies demonstrate relay acute divergent for longer-term stabilization nutrients.

Language: Английский

Citations

43
Transforming descending input into behavior: The organization of premotor circuits in theDrosophilaMale Adult Nerve Cord connectome DOI Creative Commons
Han SJ Cheong, Katharina Eichler, Tomke Stürner

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 7, 2023

Abstract In most animals, a relatively small number of descending neurons (DNs) connect higher brain centers in the animal’s head to circuits and motor (MNs) nerve cord body that effect movement limbs. To understand how signals generate behavior, it is critical these pathways are organized onto MNs. fly, Drosophila melanogaster , MNs controlling muscles leg, wing, other systems reside ventral (VNC), analogous mammalian spinal cord. companion papers, we introduced densely-reconstructed connectome Male Adult Nerve Cord (MANC, Takemura et al., 2023), including cell type developmental lineage annotation (Marin which provides complete VNC connectivity at synaptic resolution. Here, present first look organization networks connecting DNs based on this new information. We proofread curated all ensure accuracy reliability, then systematically matched DN axon terminals MN dendrites with light microscopy data link their morphology inputs or muscle targets. report both broad organizational patterns entire network fine-scale analysis selected interest. discover direct DN-MN connections infrequent identify communities intrinsic linked control different systems, putative for walking, dorsal flight steering power generation, intermediate lower tectulum coordinated action wings legs. Our generates hypotheses future functional experiments and, together MANC connectome, empowers others investigate richer mechanistic detail.

Language: Английский

Citations

38