Current Opinion in Physiology, Journal Year: 2020, Volume and Issue: 19, P. 10 - 16
Published: June 30, 2020
Language: Английский
The Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 223(4)
Published: Feb. 14, 2024
The ability to dynamically assemble contractile networks is required throughout cell physiology, yet direct biophysical mechanisms regulating non-muscle myosin 2 filament assembly in living cells are lacking. Here, we use a suite of dynamic, quantitative imaging approaches identify deterministic factors that drive appearance and amplification. We find actin dynamics regulate assembly, but the static architecture plays less clear role. Instead, remodeling modulates local monomer levels facilitates through myosin:myosin-driven interactions. Using optogenetically controlled myosin, demonstrate locally concentrating sufficient both form filaments jump-start amplification partitioning. By counting monomers within filaments, myosin-facilitated process establishes stacks prior partitioning into clusters feed higher-order networks. Together, these findings establish structures ubiquitous biology.
Language: Английский
Citations
10
Developmental Cell, Journal Year: 2024, Volume and Issue: 59(21), P. 2866 - 2881.e8
Published: Aug. 12, 2024
Formation of fluid-filled lumina by epithelial tissues is essential for organ development. How cells control the hydraulic and cortical forces to lumen morphology not well understood. Here, we quantified mechanical role tight junctions in formation using MDCK-II cysts. We found that paracellular ion barrier formed claudin receptors required inflation a lumen. However, depletion zonula occludens scaffold resulted collapse folding apical membranes. Combining quantitative measurements hydrostatic pressure junctional tension with modeling enabled us explain morphologies from pressure-tension force balance. Tight promote decreasing via inhibition myosin. In addition, our results suggest excess area contributes opening. Overall, provide understanding how use modulate tissue shape.
Language: Английский
Citations
9
Open Biology, Journal Year: 2025, Volume and Issue: 15(1)
Published: Jan. 1, 2025
Epithelia are multicellular sheets that form barriers defining the internal and external environments. The constant stresses acting at this interface require epithelial mechanically robust provide a selective barrier to hostile exterior. These properties mediated by cellular junctions which physically linked with heavily crosslinked cytoskeletal networks. Such hardwiring is facilitated plakins, family of giant modular proteins serve as ‘molecular bridges’ between different filaments multiprotein adhesion complexes. Dysfunction crosslinking compromises biomechanics structural integrity. Subsequent loss function leads disturbed tissue homeostasis pathological consequences such skin blistering or intestinal inflammation. In article, we highlight importance cytolinker protein plectin for functional organization particular, focus on ability act an integrator cytoarchitecture defines whole tissue. Finally, also discuss role in emerging aspects mechanobiology critical maintenance homeostasis.
Language: Английский
Citations
1
Current Biology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 1, 2025
Language: Английский
Citations
1
Journal of Cell Science, Journal Year: 2019, Volume and Issue: 132(22)
Published: Nov. 15, 2019
ABSTRACT Epithelial cells form tissues that generate biological barriers in the body. Tight junctions (TJs) are responsible for maintaining a selectively permeable seal between epithelial cells, but little is known about how TJs dynamically remodel response to physiological forces challenge barrier function, such as cell shape changes (e.g. during division) or tissue stretching developmental morphogenesis). In this Review, we first introduce framework think TJ remodeling across multiple scales: from molecular dynamics, strand cell- and tissue-scale dynamics. We then relate knowledge gained global perturbations of emerging information local events, where transient localized Rho activation actomyosin-mediated contraction promote repair leaks function. conclude by identifying areas field propose ideas future studies address unanswered questions mechanisms drive remodeling.
Language: Английский
Citations
72
The Journal of Cell Biology, Journal Year: 2020, Volume and Issue: 219(10)
Published: Sept. 4, 2020
Collective migration of epithelial cells plays crucial roles in various biological processes such as cancer invasion. In migrating sheets, leader form lamellipodia to advance, and follower also similar motile apparatus at cell–cell boundaries, which are called cryptic (c-lamellipodia). Using adenocarcinoma-derived cells, we investigated how c-lamellipodia found that they sporadically grew from around E-cadherin–based adherens junctions (AJs). WAVE Arp2/3 complexes were localized along the AJs, silencing them not only interfered with formation but prevented trailing leaders. Disruption AJs by removing αE-catenin resulted uncontrolled growth, this was brought about myosin II activation resultant contraction AJ-associated actomyosin cables. Additional observations indicated tended grow mechanically weak sites junction. We conclude tie together support recruiting actin regulators, enabling undergo ordered collective migration.
Language: Английский
Citations
66
Development, Journal Year: 2020, Volume and Issue: 147(5)
Published: March 1, 2020
Many developmental processes involve the emergence of intercellular fluid-filled lumina. This process luminogenesis results in a build up hydrostatic pressure and signalling molecules lumen. However, potential roles lumina cellular functions, tissue morphogenesis patterning have yet to be fully explored. In this Review, we discuss recent findings that describe how pressurized fluid expansion can provide both mechanical biochemical cues influence cell proliferation, migration differentiation. We also review emerging techniques allow for precise quantification vivo situ Finally, intricate interplay between luminogenesis, mechanics signalling, which new dimension understanding principles governing self-organization embryonic development.
Language: Английский
Citations
57
International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(14), P. 7677 - 7677
Published: July 18, 2021
The epithelial cell tight junction structure is the site of transepithelial movement solutes and water between cells (paracellular permeability). Paracellular permeability can be divided into two distinct pathways, Pore Pathway mediating small ions Leak large solutes. Claudin proteins form basic paracellular barrier mediate via Pathway. remains less understood. Several have been implicated in Pathway, including occludin, ZO proteins, tricellulin, actin filaments, but comprising remain unresolved. Many aspects such as its molecular mechanism, properties, regulation, controversial. In this review, we provide a historical background to evolution concept from initial examinations permeability. We then discuss current information about properties present theories for Finally, some recent research suggesting possible basis
Language: Английский
Citations
54
Current Biology, Journal Year: 2024, Volume and Issue: unknown
Published: Sept. 1, 2024
Language: Английский
Citations
7
eLife, Journal Year: 2019, Volume and Issue: 8
Published: Jan. 31, 2019
Cellular forces sculpt organisms during development, while misregulation of cellular mechanics can promote disease. Here, we investigate how the actomyosin scaffold protein anillin contributes to epithelial in Xenopus laevis embryos. Increased mechanosensitive recruitment vinculin cell–cell junctions when is overexpressed suggested that promotes junctional tension. However, laser ablation unexpectedly showed recoil faster depleted and slower overexpressed. Unifying these findings, demonstrate regulates medial-apical actomyosin. Medial-apical supports conclusion tensile are stored across apical surface cells, this network. Finally, show anillin’s effects on impact tissue-wide mechanics. These results reveal as a key regulator lay groundwork for future studies may contribute mechanical events development
Language: Английский
Citations
46