Force-dependent intercellular adhesion strengthening underlies asymmetric adherens junction contraction

Current Biology, Journal Year: 2022, Volume and Issue: 32(9), P. 1986 - 2000.e5

Published: April 4, 2022

Language: Английский

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EpCAM proteolysis and release of complexed claudin-7 repair and maintain the tight junction barrier

The Journal of Cell Biology, Journal Year: 2022, Volume and Issue: 222(1)

Published: Nov. 15, 2022

TJs maintain the epithelial barrier by regulating paracellular permeability. Since are under dynamically fluctuating intercellular tension, cells must continuously survey and repair any damage. However, underlying mechanisms allowing to sense TJ damage not yet fully understood. Here, we showed that proteinases play an important role in maintenance of barrier. At break sites, EpCAM-claudin-7 complexes on basolateral membrane become accessible apical membrane-anchored serine (MASPs) MASPs cleave EpCAM. Biochemical data imaging analysis suggest claudin-7 released from EpCAM contributes rapid damaged TJs. Knockout (KO) drastically reduced function live-imaging permeability MASPs-KO exhibited increased size, duration, frequency leaks. Together, our results reveal a novel mechanism through localized proteolysis at leaks, provide better understanding dynamic regulation

Language: Английский

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Staying away from the breaking point: Probing the limits of epithelial cell elimination

Current Opinion in Cell Biology, Journal Year: 2024, Volume and Issue: 86, P. 102316 - 102316

Published: Jan. 9, 2024

Epithelial tissues are dramatically remodelled during embryogenesis and tissue homeostasis yet need to maintain their sealing properties sustain barrier functions at any time. Part of these remodellings involve the elimination a large proportion cells through apoptosis. Cell extrusion, remodelling steps leading seamless dying cell expulsion, helps cohesion. However, there is an intrinsic limit in system that can only accommodate certain proportion/rate as well spatiotemporal distributions. What then critical conditions epithelial rupture/tear/sealing defects upon which mechanisms ensure such limits never reached? In this short review, I document rupture has been observed, including contexts death, mechanical parameters influencing rupture, review feedback help keep epithelia away from breaking point.

Language: Английский

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Damage control of epithelial barrier function in dynamic environments

European Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 103(2), P. 151410 - 151410

Published: April 3, 2024

Epithelial tissues cover the surfaces and lumens of internal organs multicellular animals crucially contribute to environment homeostasis by delineating distinct compartments within body. This vital role is known as epithelial barrier function. cells are arranged like cobblestones intricately bind together form an sheet that upholds this Central restriction solute fluid diffusion through intercellular spaces occluding junctions, tight junctions in vertebrates septate invertebrates. As part tissues, undergo constant renewal, with older being replaced new ones. Simultaneously, tissue undergoes relative rearrangement, elongating, shifting directionally a whole. The movement or shape changes necessitate significant deformation reconnection junctions. Recent advancements have shed light on intricate mechanisms which sustain their function dynamic environments. review aims introduce these noteworthy findings discuss some questions remain unanswered.

Language: Английский

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Actin cytoskeleton dynamics during mucosal inflammation: a view from broken epithelial barriers

Current Opinion in Physiology, Journal Year: 2020, Volume and Issue: 19, P. 10 - 16

Published: June 30, 2020

Language: Английский

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