bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 29, 2024
Summary
After
the
first
furrowing
step
of
animal
cell
division,
nascent
sibling
cells
remain
connected
by
a
thin
intercellular
bridge
(ICB).
In
isolated
siblings
migrate
away
from
each
other
to
generate
tension
and
constrict
ICB,
but
less
is
known
about
how
complete
cytokinesis
when
constrained
within
tissues.
We
examined
ICBs
formed
Drosophila
larval
brain
neural
stem
(NSC)
asymmetric
divisions
find
that
they
rely
on
constriction
focused
at
central
midbody
region
rather
than
flanking
arms
ICBs.
Super-resolution,
full
volume
imaging
revealed
unexpected
oscillatory
waves
in
plasma
membrane
sheets
surrounding
ICB
pore
during
its
formation
constriction.
these
dynamics
are
driven
Arp2/3-dependent
branched
actin
networks.
Inhibition
Arp2/3
complex
activity
blocks
oscillations
prevents
Our
results
identify
previously
unrecognized
role
for
localized
function
cannot
through
migration.
Journal of Extracellular Vesicles,
Journal Year:
2024,
Volume and Issue:
13(12)
Published: Dec. 1, 2024
Abstract
Extracellular
vesicles
(EVs)
are
heterogeneous
entities
secreted
by
cells
into
their
microenvironment
and
systemic
circulation.
Circulating
EVs
carry
functional
small
RNAs
other
molecular
footprints
from
cell
of
origin,
thus
have
evident
applications
in
liquid
biopsy,
therapeutics,
intercellular
communication.
Yet,
the
complete
transcriptomic
landscape
is
poorly
characterized
due
to
critical
limitations
including
variable
protocols
used
for
EV‐RNA
extraction,
quality
control,
cDNA
library
preparation,
sequencing
technologies,
bioinformatic
analyses.
Consequently,
there
a
gap
knowledge
need
standardized
approach
delineating
EV‐RNAs.
Here,
we
address
these
gaps
describing
following
points
(1)
focusing
on
large
canopy
particles
(EVPs),
which
includes,
but
not
limited
–
exosomes
EVs,
lipoproteins,
exomeres/supermeres,
mitochondrial‐derived
vesicles,
RNA
binding
proteins,
cell‐free
DNA/RNA/proteins;
(2)
examining
potential
roles
biogenesis
EVPs;
(3)
discussing
various
methods
technologies
uncovering
cargoes
(4)
presenting
comprehensive
list
subtypes
reported
(5)
different
databases
resources
specific
species;
(6)
reviewing
established
bioinformatics
pipelines
novel
strategies
reproducible
EV
transcriptomics
analyses;
(7)
emphasizing
significant
gold
standard
identifying
EV‐RNAs
across
studies;
(8)
finally,
highlight
current
challenges,
discuss
possible
solutions,
present
recommendations
robust
analyses
EVP‐associated
RNAs.
Overall,
seek
provide
clarity
landscape,
EVP‐RNAs.
Detailed
portrayal
state
EVP
will
lead
better
understanding
how
cargo
EVPs
can
be
modern
targeted
diagnostics
therapeutics.
For
inclusion
discussed
this
article,
use
terms
large/small
non‐vesicular
extracellular
(NVEPs),
EPs
as
defined
MISEV
guidelines
International
Society
Vesicles
(ISEV).
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: March 8, 2025
Abstract
Ribonucleoprotein
complexes
are
dynamic
assemblies
of
RNA
with
RNA-binding
proteins,
which
modulate
the
fate
RNA.
Inversely,
riboregulates
interactions
and
functions
associated
proteins.
Dysregulation
ribonucleoprotein
is
linked
to
diseases
such
as
cancer
neurological
disorders.
In
dividing
cells,
proteins
present
in
mitotic
structures,
but
their
impact
on
cell
division
remains
unclear.
By
applying
proteome-wide
R-DeeP
strategy
cells
synchronized
mitosis
versus
interphase
integrated
RBP2GO
knowledge,
we
provided
an
atlas
RNA-dependent
division,
accessible
at
R-DeeP3.dkfz.de.
We
uncovered
AURKA,
KIFC1
TPX2
unconventional
was
identified
a
new
substrate
TPX2-interacting
protein.
Their
pair-wise
were
dependent.
addition,
stimulated
AURKA
kinase
activity
stabilized
its
conformation.
this
work,
highlighted
riboregulation
major
factors
additional
complexity
level
division.
Abstract
RNAs
are
meticulously
controlled
by
proteins.
Through
direct
and
indirect
associations,
every
facet
in
the
brief
life
of
an
mRNA
is
subject
to
regulation.
R
NA‐
b
inding
p
roteins
(RBPs)
permeate
biology.
Here,
we
focus
on
their
roles
pain.
Chronic
pain
among
largest
challenges
facing
medicine
requires
new
strategies.
Mounting
pharmacologic
genetic
evidence
obtained
pre‐clinical
models
suggests
fundamental
for
a
broad
array
RBPs.
We
describe
diverse
that
span
RNA
modification,
splicing,
stability,
translation,
decay.
Finally,
highlight
opportunities
expand
our
understanding
regulatory
interactions
contribute
signaling.
This
article
categorized
under:
Interactions
with
Proteins
Other
Molecules
>
Protein‐RNA
Interactions:
Functional
Implications
Translation
Regulation
Disease
Development
The Journal of Cell Biology,
Journal Year:
2023,
Volume and Issue:
222(12)
Published: Nov. 3, 2023
Midbodies
function
during
telophase
to
regulate
the
abscission
step
of
cytokinesis.
Until
recently,
it
was
thought
that
abscission-regulating
proteins,
such
as
ESCRT-III
complex
subunits,
accumulate
at
MB
by
directly
or
indirectly
binding
resident
protein,
CEP55.
However,
recent
studies
have
shown
depletion
CEP55
does
not
fully
block
targeting
MB.
Here,
we
show
MBs
contain
mRNAs
and
these
MB-associated
can
be
locally
translated,
resulting
in
accumulation
proteins.
We
demonstrate
localized
translation
CHMP4B
is
required
for
its
site
3′
UTR-dependent
mRNA
successful
completion
Finally,
identify
regulatory
cis-elements
within
RNAs
are
necessary
sufficient
trafficking
propose
a
novel
method
regulating
cytokinesis
selective
mRNAs.
The Journal of Cell Biology,
Journal Year:
2025,
Volume and Issue:
224(7)
Published: May 12, 2025
Cytokinesis,
the
final
stage
of
cell
division,
serves
to
physically
separate
daughter
cells.
In
cultured
naïve
mouse
embryonic
stem
cells,
cytokinesis
lasts
unusually
long.
Here,
we
describe
a
novel
function
for
kinesin-13
member
KIF2A
in
this
process.
genome-engineered
find
that
localizes
spindle
poles
during
metaphase
and
regulates
length
manner
consistent
with
its
known
role
as
microtubule
minus-end
depolymerase.
contrast,
observe
tight
binding
intercellular
bridge
microtubules.
At
stage,
maintains
number
controls
acetylation.
We
propose
conversion
from
depolymerase
stabilizer
is
driven
by
both
inhibition
ATPase
activity,
which
increases
lattice
affinity,
preference
compacted
lattices.
turn,
might
maintain
state
at
bridge,
thereby
dampening
As
depletion
causes
pluripotency
problems
affects
mRNA
homeostasis,
our
results
furthermore
indicate
KIF2A-mediated
stabilization
prolongs
pluripotency.
