Membrane oscillations driven by Arp2/3 constrict the intercellular bridge during neural stem cell divisions DOI Creative Commons
Bryce LaFoya, Kenneth E. Prehoda

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 29, 2024

Summary After the first furrowing step of animal cell division, nascent sibling cells remain connected by a thin intercellular bridge (ICB). In isolated siblings migrate away from each other to generate tension and constrict ICB, but less is known about how complete cytokinesis when constrained within tissues. We examined ICBs formed Drosophila larval brain neural stem (NSC) asymmetric divisions find that they rely on constriction focused at central midbody region rather than flanking arms ICBs. Super-resolution, full volume imaging revealed unexpected oscillatory waves in plasma membrane sheets surrounding ICB pore during its formation constriction. these dynamics are driven Arp2/3-dependent branched actin networks. Inhibition Arp2/3 complex activity blocks oscillations prevents Our results identify previously unrecognized role for localized function cannot through migration.

Language: Английский

Extracellular vesicles, RNA sequencing, and bioinformatic analyses: Challenges, solutions, and recommendations DOI Creative Commons
Rebecca T. Miceli,

Tzu-yi Chen,

Yohei Nose

et al.

Journal of Extracellular Vesicles, Journal Year: 2024, Volume and Issue: 13(12)

Published: Dec. 1, 2024

Abstract Extracellular vesicles (EVs) are heterogeneous entities secreted by cells into their microenvironment and systemic circulation. Circulating EVs carry functional small RNAs other molecular footprints from cell of origin, thus have evident applications in liquid biopsy, therapeutics, intercellular communication. Yet, the complete transcriptomic landscape is poorly characterized due to critical limitations including variable protocols used for EV‐RNA extraction, quality control, cDNA library preparation, sequencing technologies, bioinformatic analyses. Consequently, there a gap knowledge need standardized approach delineating EV‐RNAs. Here, we address these gaps describing following points (1) focusing on large canopy particles (EVPs), which includes, but not limited – exosomes EVs, lipoproteins, exomeres/supermeres, mitochondrial‐derived vesicles, RNA binding proteins, cell‐free DNA/RNA/proteins; (2) examining potential roles biogenesis EVPs; (3) discussing various methods technologies uncovering cargoes (4) presenting comprehensive list subtypes reported (5) different databases resources specific species; (6) reviewing established bioinformatics pipelines novel strategies reproducible EV transcriptomics analyses; (7) emphasizing significant gold standard identifying EV‐RNAs across studies; (8) finally, highlight current challenges, discuss possible solutions, present recommendations robust analyses EVP‐associated RNAs. Overall, seek provide clarity landscape, EVP‐RNAs. Detailed portrayal state EVP will lead better understanding how cargo EVPs can be modern targeted diagnostics therapeutics. For inclusion discussed this article, use terms large/small non‐vesicular extracellular (NVEPs), EPs as defined MISEV guidelines International Society Vesicles (ISEV).

Language: Английский

Citations

16
A class of large cell-like extracellular vesicles DOI
Crislyn D’Souza‐Schorey, Dolores Di Vizio

Nature Cell Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 21, 2025

Language: Английский

Citations

1
An atlas of RNA-dependent proteins in cell division reveals the riboregulation of mitotic protein-protein interactions DOI Creative Commons

Varshni Rajagopal,

Jeanette Seiler,

Isha Nasa

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 8, 2025

Abstract Ribonucleoprotein complexes are dynamic assemblies of RNA with RNA-binding proteins, which modulate the fate RNA. Inversely, riboregulates interactions and functions associated proteins. Dysregulation ribonucleoprotein is linked to diseases such as cancer neurological disorders. In dividing cells, proteins present in mitotic structures, but their impact on cell division remains unclear. By applying proteome-wide R-DeeP strategy cells synchronized mitosis versus interphase integrated RBP2GO knowledge, we provided an atlas RNA-dependent division, accessible at R-DeeP3.dkfz.de. We uncovered AURKA, KIFC1 TPX2 unconventional was identified a new substrate TPX2-interacting protein. Their pair-wise were dependent. addition, stimulated AURKA kinase activity stabilized its conformation. this work, highlighted riboregulation major factors additional complexity level division.

Language: Английский

Citations

1
Dissecting the oncogenic properties of essential RNA-modifying enzymes: a focus on NAT10 DOI
Mahmood Hassan Dalhat, Sharath Narayan,

Hannah Serio

et al.

Oncogene, Journal Year: 2024, Volume and Issue: 43(15), P. 1077 - 1086

Published: Feb. 26, 2024

Language: Английский

Citations

5
RNA‐binding proteins in pain DOI
Patrick Smith, Zachary T. Campbell

Wiley Interdisciplinary Reviews - RNA, Journal Year: 2024, Volume and Issue: 15(2)

Published: March 1, 2024

Abstract RNAs are meticulously controlled by proteins. Through direct and indirect associations, every facet in the brief life of an mRNA is subject to regulation. R NA‐ b inding p roteins (RBPs) permeate biology. Here, we focus on their roles pain. Chronic pain among largest challenges facing medicine requires new strategies. Mounting pharmacologic genetic evidence obtained pre‐clinical models suggests fundamental for a broad array RBPs. We describe diverse that span RNA modification, splicing, stability, translation, decay. Finally, highlight opportunities expand our understanding regulatory interactions contribute signaling. This article categorized under: Interactions with Proteins Other Molecules > Protein‐RNA Interactions: Functional Implications Translation Regulation Disease Development

Language: Английский

Citations

4
The role of midbody-associated mRNAs in regulating abscission DOI Creative Commons
Trey Farmer, Katherine F. Vaeth,

Ke-Jun Han

et al.

The Journal of Cell Biology, Journal Year: 2023, Volume and Issue: 222(12)

Published: Nov. 3, 2023

Midbodies function during telophase to regulate the abscission step of cytokinesis. Until recently, it was thought that abscission-regulating proteins, such as ESCRT-III complex subunits, accumulate at MB by directly or indirectly binding resident protein, CEP55. However, recent studies have shown depletion CEP55 does not fully block targeting MB. Here, we show MBs contain mRNAs and these MB-associated can be locally translated, resulting in accumulation proteins. We demonstrate localized translation CHMP4B is required for its site 3′ UTR-dependent mRNA successful completion Finally, identify regulatory cis-elements within RNAs are necessary sufficient trafficking propose a novel method regulating cytokinesis selective mRNAs.

Language: Английский

Citations

11
Long-lived cytokinetic bridges coordinate sister-cell elimination in mouse embryos DOI
Filip Vasilev, Aleksandar I. Mihajlović, Gaudeline Rémillard‐Labrosse

et al.

Developmental Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Intercellular bridges are essential for transposon repression and meiosis in the male germline DOI Creative Commons
Julia Sorkin,

Kevin Tilton,

Matthew A. Lawlor

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 10, 2025

Language: Английский

Citations

0
The cell biology of Extracellular Vesicles: A jigsaw puzzle with a myriad of pieces DOI
Gisela D’Angelo,

Philip D. Stahl,

Graça Raposo

et al.

Current Opinion in Cell Biology, Journal Year: 2025, Volume and Issue: 94, P. 102519 - 102519

Published: April 22, 2025

Language: Английский

Citations

0
KIF2A stabilizes intercellular bridge microtubules to maintain mouse embryonic stem cell cytokinesis DOI

Lieke Stockmann,

Hélène Kabbech, Gert‐Jan Kremers

et al.

The Journal of Cell Biology, Journal Year: 2025, Volume and Issue: 224(7)

Published: May 12, 2025

Cytokinesis, the final stage of cell division, serves to physically separate daughter cells. In cultured naïve mouse embryonic stem cells, cytokinesis lasts unusually long. Here, we describe a novel function for kinesin-13 member KIF2A in this process. genome-engineered find that localizes spindle poles during metaphase and regulates length manner consistent with its known role as microtubule minus-end depolymerase. contrast, observe tight binding intercellular bridge microtubules. At stage, maintains number controls acetylation. We propose conversion from depolymerase stabilizer is driven by both inhibition ATPase activity, which increases lattice affinity, preference compacted lattices. turn, might maintain state at bridge, thereby dampening As depletion causes pluripotency problems affects mRNA homeostasis, our results furthermore indicate KIF2A-mediated stabilization prolongs pluripotency.

Language: Английский

Citations

0