Metabolic regulation of the hallmarks of stem cell biology

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(2), P. 161 - 180

Published: Feb. 1, 2024

Language: Английский

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Selective utilization of glucose metabolism guides mammalian gastrulation

Nature, Journal Year: 2024, Volume and Issue: 634(8035), P. 919 - 928

Published: Oct. 16, 2024

The prevailing dogma for morphological patterning in developing organisms argues that the combined inputs of transcription factor networks and signalling morphogens alone generate spatially temporally distinct expression patterns. However, metabolism has also emerged as a critical developmental regulator

Language: Английский

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Recording morphogen signals reveals mechanisms underlying gastruloid symmetry breaking

Nature Cell Biology, Journal Year: 2024, Volume and Issue: 26(11), P. 1832 - 1844

Published: Oct. 2, 2024

Language: Английский

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Integrated molecular-phenotypic profiling reveals metabolic control of morphological variation in a stem-cell-based embryo model

Cell stem cell, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Considerable phenotypic variation under identical culture conditions limits the potential of stem-cell-based embryo models (SEMs) in basic and applied research. The biological processes causing this seemingly stochastic remain unclear. Here, we investigated roots by parallel recording transcriptomic states morphological history individual structures modeling embryonic trunk formation. Machine learning integration time-resolved single-cell RNA sequencing with imaging-based profiling identified early features predictive end states. Leveraging power revealed that imbalance oxidative phosphorylation glycolysis results aberrant morphology a neural lineage bias, which confirmed metabolic measurements. Accordingly, interventions improved Collectively, our work establishes divergent as drivers offers broadly applicable framework to chart predict organoids SEMs. strategy can be used identify control underlying processes, ultimately increasing reproducibility.

Language: Английский

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Three‐dimensional stem cell models of mammalian gastrulation

BioEssays, Journal Year: 2024, Volume and Issue: 46(12)

Published: Aug. 28, 2024

Abstract Gastrulation is a key milestone in the development of an organism. It period cell proliferation and coordinated cellular rearrangement, that creates outline body plan. Our current understanding mammalian gastrulation has been improved by embryo culture, but there are still many open questions difficult to address because intrauterine embryos low number specimens. In case humans, additional difficulties associated with technical ethical challenges. Over last few years, pluripotent stem models being developed have potential become useful tools understand gastrulation. Here we review these special emphasis on gastruloids provide survey methods produce them robustly, their uses, relationship embryos, prospects as well limitations.

Language: Английский

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A Spatiotemporal Compartmentalization of Glucose Metabolism Guides Mammalian Gastrulation Progression

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: June 7, 2023

Gastrulation is considered the

Language: Английский

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Systematic analysis of protein stability associated with species-specific developmental tempo

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 8, 2024

Abstract Human embryonic development is generally slower compared with mouse, and one of the model systems for such inter-species differences in developmental tempo segmentation clock. The oscillation period human clock, as measured induced presomitic mesoderm (iPSM) cells, two times longer than that mouse. While core clock gene HES7 known to show protein degradation iPSM mouse iPSM, it remains unclear whether concept species-specific stability generalizable other genes. Here we systematically rates approximately 5000 genes between by using dynamic SILAC-based proteomics, demonstrating a pervasive trend cells. difference was observed not only proteasome-mediated but also lysosome-mediated degradation. We further investigated effect metabolism on profile. Treating cells glycolysis inhibitor extended period, aligning profile more closely though less These results highlight universality suggest modulators.

Language: Английский

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Historic obstacles and emerging opportunities in the field of developmental metabolism – lessons from Heidelberg

Development, Journal Year: 2024, Volume and Issue: 151(12)

Published: June 15, 2024

The field of developmental metabolism is experiencing a technological revolution that opening entirely new fields inquiry. Advances in metabolomics, small-molecule sensors, single-cell RNA sequencing and computational modeling present opportunities for exploring cell-specific tissue-specific metabolic networks, interorgan communication, gene-by-metabolite interactions time space. Together, these advances not only means by which biologists can tackle questions have challenged the centuries, but also young scientists with to define areas These emerging frontiers were at center highly interactive 2023 EMBO workshop 'Developmental metabolism: flows energy, matter, information'. Here, we summarize key discussions from this forum, emphasizing modern biology's challenges opportunities.

Language: Английский

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The logic of monsters: development and morphological diversity in stem-cell-based embryo models

Interface Focus, Journal Year: 2024, Volume and Issue: 14(5)

Published: Oct. 25, 2024

Organoids and stem-cell-based embryo models (SEMs) are imperfect organ or representations that explore a much larger space of possible forms, morphospace, compared to their

Language: Английский

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Ficus pumila fruit polysaccharide alleviates OVA-induced allergic asthma mice through the MAPK/NF-κB signaling pathway

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 105827 - 105827

Published: Jan. 1, 2025

Language: Английский

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