Elevation of ANXA1 associated with potential protective mechanism against ferroptosis and immune cell infiltration in age-related macular degeneration DOI Creative Commons
Jing Chen, Tu Lu, Chen Chen

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: Dec. 23, 2024

Age-related macular degeneration (AMD), is a neurodegenerative ocular disease. This study investigated the role of ferroptosis-related genes and their interaction with immune cell infiltration in AMD. We screened differential expression (DEGs) AMD from data sets Gene Expression Omnibus. identified differentially expressed (ferroDEGs) by intersecting DEGs genes. Protein–protein interactions network Cytoscape were used for screening hub Next, we analyzed using CIBERSORT examined crosstalk between ferroDEGs infiltration. Hub each cluster proportions different clusters normal samples single-cell data. The ferroDEG expressions verified mouse models RT–qPCR, western blot, immunofluorescence assay. roles ANXA1 ferroptosis its microglia investigated. that include six (ANXA1, DKK1, CD44, VIM, TGFB2, DUSP1). Functional analysis those was found to be correlated leukocyte migration chemotaxis, macrophage migration, gliogenesis. high-risk group exhibited elevated levels CD8+ T cells, activated NK M2 macrophages. Single-cell sequencing revealed high degree heterogeneity monocytes proportion area higher samples. Moreover, observed mRNA protein (P < 0.01), while knockdown reduced GPX4 model. Finally, validated increased colocalization assays. offers insights into pathogenesis identifies as potential target related protecting response future research.

Language: Английский

Ferroptosis: a novel mechanism of cell death in ophthalmic conditions DOI Creative Commons
Yaqi Yang,

Yumeng Lin,

Zhongyu Han

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: June 27, 2024

Ferroptosis, a new type of programmed cell death proposed in recent years, is characterized mainly by reactive oxygen species and iron-mediated lipid peroxidation differs from death, such as apoptosis, necrosis, autophagy. Ferroptosis associated with variety physiological pathophysiological processes. Recent studies have shown that ferroptosis can aggravate or reduce the occurrence development diseases targeting metabolic pathways signaling tumors, ischemic organ damage, other degenerative related to peroxidation. Increasing evidence suggests closely linked onset progression various ophthalmic conditions, including corneal injury, glaucoma, age-related macular degeneration, diabetic retinopathy, retinal detachment, retinoblastoma. Our review current research on reveals significant advancements our understanding pathogenesis, aetiology, treatment these conditions.

Language: Английский

Citations

5
Single-cell RNA sequencing highlights a significant retinal Müller glial population in dry Age-Related Macular Degeneration DOI Creative Commons
Bing Zeng,

Chuanhe Zhang,

Youcai Liang

et al.

iScience, Journal Year: 2025, Volume and Issue: 28(5), P. 112464 - 112464

Published: April 17, 2025

The main challenge in dissecting the cells and pathways involved pathogenesis of age-related macular degeneration (AMD) is highly heterogeneous dynamic nature retinal microenvironment. This study aimed to describe comprehensive landscape dry AMD (dAMD) model identify key cell cluster contributing dAMD. We identified a subset Müller that express high levels Sox2, which play crucial roles homeostasis neuroprotection both mouse models patients with Additionally, number Sox2+ decreased significantly during progression AMD, indicating these were damaged underwent death. Interestingly, ferroptosis apoptosis as contributors damage cells. Our findings are potentially valuable not only for advancing current understanding dAMD but also development treatment strategies through protection

Language: Английский

Citations

0
Improving understanding of ferroptosis: molecular mechanisms, connection with cellular senescence and implications for aging DOI Creative Commons

Diego De León-Oliva,

Diego Liviu Boaru,

Ana Minaya‐Bravo

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(21), P. e39684 - e39684

Published: Oct. 24, 2024

Language: Английский

Citations

3
Melanin‐Like Nanomedicine Functions as a Novel RPE Ferroptosis Inhibitor to Ameliorate Retinal Degeneration and Visual Impairment in Dry Age‐Related Macular Degeneration DOI

Keke Huang,

Hongkuan Deng,

Shuang Wang

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(30)

Published: Aug. 11, 2024

Ferrous ion accumulation and lethal oxidative stress mediate irreversible retinal pigment epithelial (RPE) cell ferroptosis subsequent photoreceptor degeneration, a potential key pathogenic factor in the onset of dry age-related macular degeneration (dAMD), causing vision loss global elderly population. However, currently, no effective interventional treatment strategy exists clinical practice. Herein, lesion site-targeted melanin-like nanoparticles, named ConA-MelNPs, are designed as novel inhibitor for degenerative diseases. ConA-MelNPs possessed chelating iron characteristics, alleviating severe mitochondrial damage caused by protecting RPE cells from induced sodium iodate (NaIO

Language: Английский

Citations

2
Astragaloside IV attenuates ferroptosis and protects against iron overload-induced retinal injury DOI
Qiang Li, Yuanyuan Zhang, Pan Liu

et al.

Experimental Eye Research, Journal Year: 2024, Volume and Issue: 246, P. 110021 - 110021

Published: Aug. 6, 2024

Language: Английский

Citations

2
Uncovering the role of ferroptosis in Bietti crystalline dystrophy and potential therapeutic strategies DOI Creative Commons

Chang Shen,

Qianjie Yang,

Kuangqi Chen

et al.

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: July 11, 2024

Abstract Purpose Bietti crystalline dystrophy (BCD) is an inherited retinal degeneration disease caused by mutations in the CYP4V2 gene. Currently, there no clinical therapy approach available for BCD patients. Previous research has suggested that polyunsaturated fatty acids (PUFAs) may play a significant role development of BCD, implicating involvement ferroptosis pathogenesis. In this work, we aimed to investigate interplay between and detect potential therapeutic strategies disease. Methods Genetic-edited RPE cell line was first established study CRISPR-Cas9 technology. Cyp4v3 (the homologous gene human CYP4V2) knock out (KO) mice have also been used. Lipid profiling transcriptome analysis pigment epithelium (RPE) cells from KO conducted. Ferroptosis phenotypes investigated models vitro vivo, including lipid peroxidation, mitochondrial changes, elevated levels reactive oxygen species (ROS), altered expression. Additionally, iron chelator, deferiprone (DFP), tested vivo determine its efficacy suppressing restoring phenotype. Results exhibited progressive accumulation, similar phenotype, which exacerbated high-fat diet (HFD). Increased PUFAs, such as EPA (C22:5) AA (C20:4), were observed mice. Transcriptome revealed changes genes involved homeostasis, particularly upregulation NCOA4, confirmed immunofluorescence. Ferroptosis-related characteristics, defects, ROS related genes, detected both vivo. Abnormal accumulation ferrous detected. DFP, chelator administration suppressed phenotype mutated RPE. Oral DFP restored function morphology Conclusion This represented evidence substantial BCD. PUFAs resulting mutation serve substrates ferroptosis, potentially working conjunction with NCOA4-regulated ultimately leading degeneration. administration, chelates iron, demonstrated ability reverse suggesting promising future.

Language: Английский

Citations

1
Serum Iron Status and Retinal Degenerative Diseases: A Mendelian Randomization Study on AMD, RP, and DR DOI Open Access
Sichang Qu,

Yewen Zhu,

Norbert Pfeiffer

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(21), P. 3747 - 3747

Published: Oct. 31, 2024

: Observational studies have noted that patients with certain retinal degenerative diseases exhibit iron disturbances in the retina or vitreous compared to healthy controls. However, connection between serum status and these remains unclear. This study aims explore potential causal relationship biomarkers development of age-related macular degeneration (AMD), retinitis pigmentosa (RP), diabetic retinopathy (DR).

Language: Английский

Citations

0
Precision mitochondrial medicine: Uncovering pathways across diverse diseases DOI
Lourdes Sainero‐Alcolado, Elisa Garde-Lapido, Judit Liaño-Pons

et al.

Elsevier eBooks, Journal Year: 2024, Volume and Issue: unknown, P. 115 - 169

Published: Nov. 29, 2024

Language: Английский

Citations

0
Elevation of ANXA1 associated with potential protective mechanism against ferroptosis and immune cell infiltration in age-related macular degeneration DOI Creative Commons
Jing Chen, Tu Lu, Chen Chen

et al.

European journal of medical research, Journal Year: 2024, Volume and Issue: 29(1)

Published: Dec. 23, 2024

Age-related macular degeneration (AMD), is a neurodegenerative ocular disease. This study investigated the role of ferroptosis-related genes and their interaction with immune cell infiltration in AMD. We screened differential expression (DEGs) AMD from data sets Gene Expression Omnibus. identified differentially expressed (ferroDEGs) by intersecting DEGs genes. Protein–protein interactions network Cytoscape were used for screening hub Next, we analyzed using CIBERSORT examined crosstalk between ferroDEGs infiltration. Hub each cluster proportions different clusters normal samples single-cell data. The ferroDEG expressions verified mouse models RT–qPCR, western blot, immunofluorescence assay. roles ANXA1 ferroptosis its microglia investigated. that include six (ANXA1, DKK1, CD44, VIM, TGFB2, DUSP1). Functional analysis those was found to be correlated leukocyte migration chemotaxis, macrophage migration, gliogenesis. high-risk group exhibited elevated levels CD8+ T cells, activated NK M2 macrophages. Single-cell sequencing revealed high degree heterogeneity monocytes proportion area higher samples. Moreover, observed mRNA protein (P < 0.01), while knockdown reduced GPX4 model. Finally, validated increased colocalization assays. offers insights into pathogenesis identifies as potential target related protecting response future research.

Language: Английский

Citations

0