Functional MRI and Tumor Vasculature Correlation in Ewing Sarcoma Xenografts: A Prospective Study Based on MRI–Pathology Co‐Alignment DOI Open Access
X. Liu, Kai Zhang, Yutong Song

et al.

Journal of Magnetic Resonance Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Limited studies have evaluated vascular markers of Ewing sarcoma (ES) using MRI. To explore the correlation between tumor and MRI perfusion parameters in ES xenografts based on MRI-pathology co-alignment. Prospective. Thirty-four xenograft models were established female athymic nude mice human-derived A673 cell line. 3 T MRI, T1-weighted (T1w) with fast spin echo sequence, T2w recovery intravoxel incoherent motion (IVIM) echo-planar diffusion-weighted dynamic contrast-enhanced (DCE-MRI) liver acquisition volume acceleration sequence. IVIM (D, D*, f), DCE-MRI semiquantitative (maximum slope increase [MSI], contrast-enhancement ratio [CER], initial area under gadolinium curve [iAUGC]), quantitative (Ktrans, Kep, Ve). The expression endothelial growth factor (VEGF), microvessel density (MVD), mimicry (VM) was by immunohistochemical staining. Intraclass coefficient (ICC), bootstrap resampling, Fisher's Z transformation, Pearson or Spearman analysis, receiver operating characteristic (ROC) DeLong's test. p < 0.05 considered statistically significant. Ktrans, f, D* values showed significant correlations VEGF (r = 0.697, 0.630, 0.781, 0.695, respectively). MSI, CER MVD 0.42, 0.554, 0.486, 0.461, 0.416, D f VM -0.552, 0.384, Kep good diagnostics distinguishing high- low-expression groups (AUC 0.833-0.954). 0.727, 0.739, can be utilized to assess vasculature xenografts. 1. Stage 3.

Language: Английский

Targeting PI3K/AKT/mTOR Signaling to Overcome Drug Resistance in Cancer DOI
Muhammad Tufail,

Wendong Wan,

Canhua Jiang

et al.

Chemico-Biological Interactions, Journal Year: 2024, Volume and Issue: 396, P. 111055 - 111055

Published: May 17, 2024

Language: Английский

Citations

10
CircDCAF8 promotes the progression of hepatocellular carcinoma through miR-217/NAP1L1 Axis, and induces angiogenesis and regorafenib resistance via exosome-mediated transfer DOI Creative Commons

Jiahao Gong,

Guoyong Han,

Zhiqiang Chen

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: May 30, 2024

Abstract Background Circular RNAs (circRNAs), which are a new type of single-stranded circular RNA, have significant involvement in progression many diseases, including tumors. Currently, multiple circRNAs been identified hepatocellular carcinoma (HCC). Our study aims to investigate the function and mechanism circDCAF8 HCC. Methods The expression (hsa_circ_0014879) HCC para-carcinoma tissue samples was determined using quantitative real-time polymerase chain reaction (qRT-PCR). biological confirmed by experiments conducted both vitro vivo. And relationship between circDCAF8, miR-217 NAP1L1 predicted database verified qRT-PCR, RNA-binding protein immunoprecipitation (RIP) dual-luciferase reporter assays. Exosomes isolated from cells were utilized assess connection exosomal with angiogenesis regorafenib resistance. Results CircDCAF8 is upregulated tissues cell lines, linked an unfavourable prognosis for patients. Functionally, proved facilitate proliferation, migration, invasion Epithelial-Mesenchymal Transformation (EMT) cells. Animal examinations also validated tumor-promoting characteristics on Besides, promoted HUVECs. Mechanistically, interacted downstream miR-217. sponging In addition, exosomes may transfer regorafenib-resistant sensitive cells, where it would confer resistant phenotype. Conclusion facilitates proliferation metastasis via miR-217/NAP1L1 axis. Meanwhile, can promote drive resistance regorafenib, making viable therapeutic target

Language: Английский

Citations

10
Cellular Dynamics of Tumor Microenvironment Driving Immunotherapy Resistance in Non-Small-Cell Lung Carcinoma DOI
Shujie Huang, Jeff Yat‐Fai Chung, Chunjie Li

et al.

Cancer Letters, Journal Year: 2024, Volume and Issue: unknown, P. 217272 - 217272

Published: Sept. 1, 2024

Language: Английский

Citations

8
Unveiling the Anti-Angiogenic Potential of Small-Molecule (Kinase) Inhibitors for Application in Rheumatoid Arthritis DOI Creative Commons

Fatemeh Khodadust,

Eva M. L. Philippon,

Maarten M. Steinz

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(2), P. 102 - 102

Published: Jan. 11, 2025

Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by inflammation leading to joint damage and systemic complications. Angiogenesis promotes contributes RA progression. This study evaluated potential anti-angiogenic effects of several compounds including small-molecule kinase inhibitors, such as sunitinib (pan-kinase inhibitor), tofacitinib (JAK-inhibitor), NIKi (NF-κB-inducing the integrin-targeting peptide fluciclatide, using scratch assay 3D spheroid-based models angiogenesis. For all drugs tested in low micromolar range (1–25 μM), (as positive anti-angiogenetic control) showed marked inhibition endothelial cell (EC) migration sprouting, effectively reducing both closure sprout formation. Tofacitinib exhibited marginal effectiveness assay, but performed better (55% inhibition), whereas around 50% models. Fluciclatide changed EC morphology rather than migration, only when stimulated with synovial fluid spheroid model did it show inhibitory (at ≥2.5 µM), none below this dosage. These results highlight for angiogenesis fluciclatide safe diagnostic targeting microdose RA, well need advanced screening that mimic RA’s complex inflammatory pro-angiogenic environment.

Language: Английский

Citations

1
Functional MRI and Tumor Vasculature Correlation in Ewing Sarcoma Xenografts: A Prospective Study Based on MRI–Pathology Co‐Alignment DOI Open Access
X. Liu, Kai Zhang, Yutong Song

et al.

Journal of Magnetic Resonance Imaging, Journal Year: 2025, Volume and Issue: unknown

Published: March 20, 2025

Limited studies have evaluated vascular markers of Ewing sarcoma (ES) using MRI. To explore the correlation between tumor and MRI perfusion parameters in ES xenografts based on MRI-pathology co-alignment. Prospective. Thirty-four xenograft models were established female athymic nude mice human-derived A673 cell line. 3 T MRI, T1-weighted (T1w) with fast spin echo sequence, T2w recovery intravoxel incoherent motion (IVIM) echo-planar diffusion-weighted dynamic contrast-enhanced (DCE-MRI) liver acquisition volume acceleration sequence. IVIM (D, D*, f), DCE-MRI semiquantitative (maximum slope increase [MSI], contrast-enhancement ratio [CER], initial area under gadolinium curve [iAUGC]), quantitative (Ktrans, Kep, Ve). The expression endothelial growth factor (VEGF), microvessel density (MVD), mimicry (VM) was by immunohistochemical staining. Intraclass coefficient (ICC), bootstrap resampling, Fisher's Z transformation, Pearson or Spearman analysis, receiver operating characteristic (ROC) DeLong's test. p < 0.05 considered statistically significant. Ktrans, f, D* values showed significant correlations VEGF (r = 0.697, 0.630, 0.781, 0.695, respectively). MSI, CER MVD 0.42, 0.554, 0.486, 0.461, 0.416, D f VM -0.552, 0.384, Kep good diagnostics distinguishing high- low-expression groups (AUC 0.833-0.954). 0.727, 0.739, can be utilized to assess vasculature xenografts. 1. Stage 3.

Language: Английский

Citations

1