Emergence of hypervirulent and carbapenem-resistant Klebsiella pneumoniae from 2014 - 2021 in Central and Eastern China: a molecular, biological, and epidemiological study

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Nov. 11, 2024

In recent years, the hypervirulent and carbapenem-resistant Klebsiella pneumoniae has been increasingly reported worldwide. The objective of this study was to compare antibiotic resistance virulence profiles K.pneumoniae (CR-hvKP) (hv-CRKP) identify prevailing strain in clinical settings.

Language: Английский

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Poor Glycemic Control in Carbapenem-Resistant Klebsiella pneumoniae Infections: Impact on Epidemiological Features, Mortality Risks, and Polymyxin Resistance

Infection and Drug Resistance, Journal Year: 2025, Volume and Issue: Volume 18, P. 647 - 660

Published: Feb. 1, 2025

Purpose: This study aims to investigate the relationship between glycemic control and epidemiological characteristics of patients infected with carbapenem-resistant Klebsiella pneumoniae (CRKP), identify mortality risk factors associated CRKP infection, evaluate impact glucose on resistance polymyxin serum killing. Patients Methods: Clinical cases 218 were collected from a large tertiary public hospital in Anhui Province. We analyzed whether impacts clinical laboratory manifestations patients. Logistic regression identified factors. Antibiotic sensitivity, capsular serotypes, virulence genes tested strains. Three clinically isolated strains used effect bacterial capsule synthesis Results: poor experienced more severe infections had higher likelihood chronic kidney disease (CKD) acute renal insufficiency compared those good control. They also exhibited an increased rate. analysis age, glycosylated hemoglobin (HbA 1c ) ≥ 7%, CKD, tumor, mechanical ventilation, sepsis as independent for death infection. A 0.5% (0.5 g/100mL) environment can stimulate synthesis, which is inhibitable by cyclic adenosine monophosphate (cAMP). Moreover, high-glucose enhance CRKP's Conclusion: persistent hyperglycemic resulting may capsules, could killing, thereby further increasing patient mortality. Keywords: , control, polymyxin, resistance, capsule, diabetes

Language: Английский

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Molecular epidemiology and genetic dynamics of carbapenem-resistant hypervirulent Klebsiella pneumoniae in China

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 14, 2025

Carbapenem-resistant hypervirulent Klebsiella pneumoniae (CRhvKP) poses a significant global health threat due to its enhanced virulence and resistance. This study analyzed 5,036 publicly available K. genomes from China (2005–2023), identifying 1,538 CRhvKP genomes, accounting for 44.6% of carbapenem-resistant isolates 69.5% isolates. Predominant carbapenemases included bla KPC (92.1%), with an increasing prevalence NDM OXA-48-like genes. Most (93.6%) carried both aerobactin yersiniabactin The genetic background showed high diversity, characterized by 36 sequence types (STs) 22 capsule types, high-risk endemic STs such as ST11, ST15, ST23 being predominant. demonstrated virulence, whereas ST11 more resistance genes but minimal presence iroBCDN A core genome MLST analysis revealed that 89.0% clustered into 131 clonal groups, indicating widespread dissemination, particularly in eastern China. CR hv plasmids, primarily IncF, IncH, IncR distinct community structures, plasmids demonstrating higher mobility diversity. Crucially, we identified 40 CR-hv convergent across five STs, likely resulting plasmid fusions, which have become increasingly prevalent over the last decade. Furthermore, chromosomal integration KPC-2 was detected, underscoring stable inheritance these traits. Class 1 Integrons were present 84.5% strains, most notably least ST23. These integrons harbored confer various antibiotics, including IMP VIM , their content varying different STs. highlights complexity, rapid China, emphasizing urgent need genomic surveillance targeted interventions mitigate posed multidrug-resistant strains.

Language: Английский

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Epidemiological, phenotypic and genotypic characteristics difference of hypervirulent and carbapenem-resistant Klebsiella pneumoniae with different capsular serotypes

Journal of Microbiology Immunology and Infection, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

KL1, KL2 CR-hvKP and KL64, KL47 hv-CRKP all exhibit overlapping multidrug resistance hypervirulence phenotypes, but the differences in epidemiological, phenotypic genotypic characteristics between them remains unclear. In this study, we collected non-repeated hv-CRKP/CR-hvKP isolates a tertiary hospital Shanghai, China from January 2019 to December 2022. Furthermore, selected four typical hypervirulent carbapenem-resistant Klebsiella pneumoniae, including ST23-KL1/ST86-KL2 (WYKP3 WYKP194) ST11-KL64/ST11-KL47 (WYKP589 WYKP188), tried clarify compare their virulence drug plasmid distribution. Our study found that ST23-KL1 ST86-KL2 exhibited less diversity than of ST11-KL64 ST11-KL47 hv-CRKP. Compared with hv-CRKP, harbored significantly fewer antimicrobial genes more genes, which contributed higher these strains antibiotics. has emerged as most prevalent pneumoniae probably due its clonal transmission within hospitals well plasmids help conjugative plasmids. Due different evolutionary mechanisms capsular serotypes, are different.

Language: Английский

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Zebrafish as a model for investigating Klebsiella pneumoniae -driven lung injury and therapeutic targets

Experimental Lung Research, Journal Year: 2025, Volume and Issue: 51(1), P. 11 - 22

Published: March 5, 2025

Background: Lung injury induced by Klebsiella pneumoniae infection presents a significant challenge, with complex molecular mechanisms driving tissue damage and immune dysregulation. This study aimed to establish zebrafish model of K. pneumoniae-induced lung explore the underlying involved in damage, responses, development. Methods: A was developed injecting into swim bladder at 96 h post-fertilization (hpf). The response, including neutrophil migration cytokine secretion, assessed through histological analysis quantitative measures. Transcriptomic performed evaluate gene expression changes related development, regulation, metabolism. role TGF-β signaling pathway response repair investigated using inhibitor SB 431542. Results: Infection rapid secretion inflammatory cytokines such as IL-6, IL-1β, TNF-α, TNF-β, similar responses seen mouse models. revealed alterations genes metabolic pathways, underscoring broad impact on physiological regulation. found play dual role: it promoted cell recruitment but suppressed developmental genes, delaying repair. Treatment 431542 reduced aggregation, lowered levels, restored development Conclusions: effectively mimics injury, offering valuable insights Targeting holds therapeutic potential for reducing inflammation promoting repair, providing foundation new treatment strategies infections.

Language: Английский

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Carbapenem-resistant hypervirulent Klebsiella pneumoniae: where is it headed in the tug-of-war between virulence and resistance?

EBioMedicine, Journal Year: 2025, Volume and Issue: 114, P. 105649 - 105649

Published: March 10, 2025

Language: Английский

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Identification of a novel phage depolymerase against ST11 K64 carbapenem-resistant Klebsiella pneumoniae and its therapeutic potential

Journal of Bacteriology, Journal Year: 2025, Volume and Issue: unknown

Published: March 26, 2025

ABSTRACT Carbapenem-resistant Klebsiella pneumoniae (CRKP) is a clinical pathogen with high mortality rate, and its management infection control have become serious challenge. Phage-encoded depolymerase cleaves the capsular polysaccharide, major virulence factor of K. . This study aimed to identify phage targeting ST11 K64 CRKP, evaluate antimicrobial activity therapeutic efficacy, provide new alternative strategies for CRKP. Phages were screened from untreated hospital sewage using clinically isolated CRKP as host bacterium. The range, efficiency plaque formation, optimal multiplicity infection, adsorption efficiency, one-step growth curve vB_KpnP_IME1309 determined by double-layer agar plate culture method. morphology was observed transmission electron microscopy. Phage nucleic acids extracted whole-genome sequencing, phage-encoded gene ORF37 amplified polymerase chain reaction. Next, recombinant plasmid constructed induce expression, which verified sodium dodecyl sulfate-polyacrylamide gel electrophoresis. In vitro bactericidal combined serum assay, anti- K biofilm effect crystal violet staining. Finally, Galleria mellonella larvae model established investigate on in vivo Here, we characterized featured latent period 20 min burst size approximately 290 plaque-forming units/cell. It contained 41 predicted open reading frames, encoded depolymerase. expressed purified Dep37 cleaved only formed translucent halo plate. increased susceptibility B1 killing, inhibited degraded mature biofilms. combination kanamycin significantly more effective treating biofilms compared either or alone. An injection at 5 2 h after their survival rates up 73% 53%, respectively. Depolymerase may be used potential method capsule typing , showing great promise development novel against IMPORTANCE A carbapenem-resistant characterized. encoding successfully purified. increases inhibits degrades than alone treatment biofilm. rate

Language: Английский

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Molecular epidemiological analysis and research on resistance and virulence of carbapenem-resistant Klebsiella pneumoniae in a tertiary hospital from 2016 to 2023

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 16, 2025

Carbapenem-resistant Klebsiella pneumoniae (CRKP) represents a significant global threat due to its high prevalence rates and limited therapeutic options. The primary objective of this study was investigate the clinical distribution molecular epidemiology CRKP collected between 2016 2023 from tertiary care hospital in northern China. Polymerase chain reaction (PCR) assays were used identify resistance virulence genes, while various assessments, including string test biofilm formation analysis, assessed CRKP's virulence. Multilocus sequence typing (MLST) whole-genome sequencing employed elucidate strain classification plasmid characteristics. identified 100 unique strains, primarily isolated neurosurgery ICU, with sputum as most common specimen type. majority strains harbored blaKPC-2 mechanism. All minimum four entB, mrkD, fimH, ybtS being commonly detected across isolates. Notably, 66 classified carbapenem-resistant hypervirulent (CR-hvKP). prevailing type (ST) observed ST11, serotype KL47 prevalent initially, subsequently supplanted by ST11-KL64. Specific on IncFII-type plasmids, along other such blaTEM-1. KP635_PlasmidB harbors multiple antibiotic identity coverage KP635_PlasmidA NTUH-K2044 are 99%, which contributes highly virulent multidrug-resistant KP635. emergence hypervirulence requires vigilance, enhanced surveillance, stringent infection control measures limit spread.

Language: Английский

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A one-pot, one-step CRISPR-AapCas12b-based platform for sensitive and specific detection of Klebsiella pneumoniae

Sensing and Bio-Sensing Research, Journal Year: 2025, Volume and Issue: unknown, P. 100797 - 100797

Published: April 1, 2025

Language: Английский

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Emergence of High-Level Antibiotic Resistance in Klebsiella pneumoniae: A Narrative Review

South Asian Journal of Research in Microbiology, Journal Year: 2024, Volume and Issue: 18(2), P. 1 - 8

Published: Feb. 2, 2024

In an era marked by remarkable advancements in medicine, the persistent emergence of high-level antibiotic resistance Klebsiella pneumoniae poses a critical threat to public health globally. As worldwide spread extensively drug-resistant (XDR) and pan-drug-resistant (PDR) K. strains continues grow, significant shift how we approach treatment is on horizon. The complex interaction genetic factors, which encompasses wide range beta-lactamases, aminoglycoside-modifying enzymes, chromosomal mutations, creates dynamic mechanism that counters effects antibiotics. These intricate adaptations, arising from both gene transfers facilitated plasmids changes genome itself, present challenging obstacle our efforts managing antimicrobial effectiveness. infections come back stronger armed with molecular tactics challenge healthcare systems, prolong hospital stays, increase mortality. Beyond settings, economic social dimensions grow as resources are redirected, intensifying impact vulnerable groups. This review delves into mechanisms behind pneumoniae, examining its epidemiological, molecular, clinical facets. Highlighting necessity for coordinated research, medical protocols, policies, underscores importance judicious utilization, drug innovation, rigorous infection management.

Language: Английский

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