Treated wastewater: A hotspot for multidrug- and colistin-resistant Klebsiella pneumoniae DOI Creative Commons
Andrea Di Cesare, Alessandra Cornacchia, Tomasa Sbaffi

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 359, P. 124598 - 124598

Published: July 23, 2024

Wastewater treatment plants are hotspots for the release of antimicrobial resistant pathogenic bacteria into aquatic ecosystems, significantly contributing to cycle resistance. Special attention should be paid ESKAPE bacteria, which have been identified as high-priority targets control measures. Among them, Klebsiella pneumoniae is particularly noteworthy. In this study, we collected wastewater samples from inlet, sedimentation tank, and effluent water a plant in June, July, October, November 2018. We detected characterized 42 K. strains using whole genome sequencing (15 8 19 effluent). Additionally, were tested their resistance phenotype. Using no distinct patterns observed terms genetic profiles. All tetracycline, meanwhile 60%, 47%, 37.5% isolated effluent, respectively, multidrug resistant. Some isolates also colistin, nearly all positive eptB arnT genes, associated with polymyxin Various genes linked mobile elements, they did not correlate virulence groups or defense systems. Overall, our results, although quantitative, highlight that strains, including those colistin genetically unrelated, being discharged ecosystems plants. This suggests necessity monitoring aimed at characterizing these bacteria.

Language: Английский

In-depth characterization of multidrug-resistant NDM-1 and KPC-3 co-producing Klebsiella pneumoniae bloodstream isolates from Italian hospital patients DOI Creative Commons
Brunella Posteraro, Flavio De Maio, Yair Motro

et al.

Microbiology Spectrum, Journal Year: 2024, Volume and Issue: 12(4)

Published: Feb. 27, 2024

Bloodstream infection (BSI) caused by carbapenem-resistant

Language: Английский

Citations

5
Assessment of antimicrobial resistance, biofilm formation, and surface modification potential in hospital strains of Pseudomonas aeruginosa and Klebsiella pneumoniae DOI Creative Commons

Roberta Lordelo,

Rita Branco,

Fernando Gama

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(9), P. e30464 - e30464

Published: April 27, 2024

The occurrence of healthcare-associated infections is a multifactorial phenomenon related to hospital space contamination by bacteria. ESKAPE group, specifically Pseudomonas aeruginosa and Klebsiella pneumoniae, play relevant role in the these infections. Therefore, comprehensive research needed identify characteristics that justify prevalence species healthcare environment. In this line, study aimed determine antimicrobial resistance, biofilm formation, potential for polymer degradation collection 33 P. strains 2 K. pneumoniae sampled from various equipment non-critical surfaces Portuguese hospital. Antimicrobial susceptibility tests revealed none was categorized as multidrug-resistant (non-MDR). An assessment their biofilm-forming capabilities indicated 97 % exhibited biofilm-producing characteristics. Notably, within majority half were classified strong producers. Furthermore, evaluated cause damage or change medical devices, namely infusion sets, nasal cannula, urinary catheters. Three strains, two one moderate producers, showed highest ability modify cannula sets. Additionally, Chi-square test statistically significant relationship between presence water accession spots. conclusion, work suggests bacteria group hold grow environment through materials. This concern persistence proliferation organisms environments, emphasizing importance robust infection control measures mitigate risks associated with bacterial growth on such surfaces.

Language: Английский

Citations

5
Should multidrug resistant Klebsiella pneumoniae strains displaying hypervirulent traits be reclassified as either ultravirulent or supervirulent? DOI Creative Commons
Bruno Douradinha

Microbiological Research, Journal Year: 2023, Volume and Issue: 275, P. 127446 - 127446

Published: July 6, 2023

Language: Английский

Citations

12
A new anti-CRISPR gene promotes the spread of drug-resistance plasmids in Klebsiella pneumoniae DOI Creative Commons
Chunyu Jiang, Chengzhi Yu,

Shuyi Sun

et al.

Nucleic Acids Research, Journal Year: 2024, Volume and Issue: 52(14), P. 8370 - 8384

Published: June 18, 2024

The Klebsiella pneumoniae (K. pneumoniae, Kp) populations carrying both resistance-encoding and virulence-encoding mobile genetic elements (MGEs) significantly threaten global health. In this study, we identified a new anti-CRISPR gene (acrIE10) on conjugative plasmid with self-target sequence in K. type I-E* CRISPR-Cas system. AcrIE10 interacts the Cas7* subunit of crystal structure AcrIE10-KpCas7* complex suggests that suppresses by binding directly to Cas7 prevent its hexamerization, thereby preventing surveillance assembly crRNA loading. Bioinformatic functional analyses revealed is functionally widespread across diverse species. Our study reports novel highlights potential role spreading resistance virulence among pathogens.

Language: Английский

Citations

4
Treated wastewater: A hotspot for multidrug- and colistin-resistant Klebsiella pneumoniae DOI Creative Commons
Andrea Di Cesare, Alessandra Cornacchia, Tomasa Sbaffi

et al.

Environmental Pollution, Journal Year: 2024, Volume and Issue: 359, P. 124598 - 124598

Published: July 23, 2024

Wastewater treatment plants are hotspots for the release of antimicrobial resistant pathogenic bacteria into aquatic ecosystems, significantly contributing to cycle resistance. Special attention should be paid ESKAPE bacteria, which have been identified as high-priority targets control measures. Among them, Klebsiella pneumoniae is particularly noteworthy. In this study, we collected wastewater samples from inlet, sedimentation tank, and effluent water a plant in June, July, October, November 2018. We detected characterized 42 K. strains using whole genome sequencing (15 8 19 effluent). Additionally, were tested their resistance phenotype. Using no distinct patterns observed terms genetic profiles. All tetracycline, meanwhile 60%, 47%, 37.5% isolated effluent, respectively, multidrug resistant. Some isolates also colistin, nearly all positive eptB arnT genes, associated with polymyxin Various genes linked mobile elements, they did not correlate virulence groups or defense systems. Overall, our results, although quantitative, highlight that strains, including those colistin genetically unrelated, being discharged ecosystems plants. This suggests necessity monitoring aimed at characterizing these bacteria.

Language: Английский

Citations

4