bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 22, 2025
Abstract
The
widespread
application
of
high-throughput
Next
Generation
Sequencing
(NGS)
technologies
has
made
microbiome
research
an
emerging
field
in
public
health
and
biomedical
sciences.
However,
there
are
still
many
challenges
that
need
to
be
addressed
this
field.
Pipelines
available
generate
data
across
cohorts
diverse,
sources
variation
recorded
evaluated
during
profiling
have
not
been
standardized.
Moreover,
meticulous
quality
control
the
processing,
from
collection
computational
quantification
is
challenging,
especially
large
population
studies.
Innovative
approaches
required
handle
samples
minimize
potential
bias
introduced
by
logistic
hurdles
biobanking.
In
paper,
we
describe
methodological
steps
surrounding
optimization
16s
rRNA
gut
two
prospective
R
Study
(mean
age
9.83
±
0.32
years)
Rotterdam
62.67
5.66
years).
This
paper
also
highlights
solutions
sample
mislabeling
large-scale
analysis.
To
summarize,
our
study
addresses
common
problems
human
research.
It
aims
improve
reliability
integrating
more
stringent
standards
into
Nutrients,
Journal Year:
2024,
Volume and Issue:
16(16), P. 2657 - 2657
Published: Aug. 11, 2024
Irritable
bowel
syndrome
(IBS)
is
a
prevalent
functional
gastrointestinal
disorder
characterized
by
abdominal
pain,
bloating,
diarrhea,
and
constipation.
Recent
studies
have
underscored
the
significant
role
of
gut
microbiota
in
pathogenesis
IBS.
Physical
exercise,
as
non-pharmacological
intervention,
has
been
proposed
to
alleviate
IBS
symptoms
modulating
microbiota.
Aerobic
such
running,
swimming,
cycling,
shown
enhance
diversity
abundance
beneficial
bacteria,
including
Lactobacillus
Bifidobacterium.
These
bacteria
produce
short-chain
fatty
acids
that
possess
anti-inflammatory
properties
support
barrier
integrity.
Studies
involving
patients
participating
structured
aerobic
exercise
programs
reported
improvements
their
microbiota's
composition
diversity,
alongside
an
alleviation
like
pain
bloating.
Additionally,
positively
influences
mental
health
reducing
stress
improving
mood,
which
can
further
relieve
via
gut-brain
axis.
Long-term
interventions
provide
sustained
benefits,
maintaining
stability,
supporting
immune
functions,
systemic
inflammation.
However,
must
be
tailored
individual
needs
avoid
exacerbating
symptoms.
Personalized
plans
starting
with
low-to-moderate
intensity
gradually
increasing
maximize
benefits
minimize
risks.
This
review
examines
impact
various
types
intensities
physical
on
patients,
highlighting
need
for
explore
optimal
protocols.
Future
research
should
include
larger
sample
sizes,
longer
follow-up
periods,
examine
synergistic
effects
other
lifestyle
modifications.
Integrating
into
comprehensive
management
symptom
control
improve
patients'
quality
life.
International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(11), P. 5782 - 5782
Published: May 26, 2024
Nowadays,
depressive
disorder
is
spreading
rapidly
all
over
the
world.
Therefore,
attention
to
studies
of
pathogenesis
disease
in
order
find
novel
ways
early
diagnosis
and
treatment
increasing
among
scientific
medical
communities.
Special
drawn
a
biomarker
therapeutic
strategy
through
microbiota–gut–brain
axis.
It
known
that
symbiotic
interactions
between
gut
microbes
host
can
affect
mental
health.
The
review
analyzes
mechanisms
action
microbiota
on
pathophysiology
depression.
possibility
using
knowledge
about
taxonomic
composition
metabolic
profile
patients
with
depression
select
gene
compositions
(metagenomic
signature)
as
biomarkers
evaluated.
use
silico
technologies
(machine
learning)
for
based
given.
Alternative
approaches
are
being
considered
by
balancing
microbial
dietary
modifications
additives,
namely
probiotics,
postbiotics
(including
vesicles)
prebiotics
psychobiotics,
fecal
transplantation.
bacterium
Faecalibacterium
prausnitzii
under
consideration
promising
new-generation
probiotic
auxiliary
diagnostic
analysis
conducted
this
may
be
useful
clinical
practice
pharmacology.
Life,
Journal Year:
2024,
Volume and Issue:
14(10), P. 1234 - 1234
Published: Sept. 26, 2024
Microbes
have
inhabited
the
earth
for
hundreds
of
millions
years
longer
than
humans.
The
microbiota-gut-brain
axis
(MGBA)
represents
a
bidirectional
communication
pathway.
These
communications
occur
between
central
nervous
system
(CNS),
enteric
(ENS),
and
emotional
cognitive
centres
brain.
field
research
on
gut-brain
has
grown
significantly
during
past
two
decades.
Signalling
occurs
gut
microbiota
brain
through
neural,
endocrine,
immune,
humoral
pathways.
A
substantial
body
evidence
indicates
that
MGBA
plays
pivotal
role
in
various
neurological
diseases.
include
Alzheimer's
disease
(AD),
autism
spectrum
disorder
(ASD),
Rett
syndrome,
attention
deficit
hyperactivity
(ADHD),
non-Alzheimer's
neurodegeneration
dementias,
fronto-temporal
lobe
dementia
(FTLD),
Wilson-Konovalov
(WD),
multisystem
atrophy
(MSA),
Huntington's
chorea
(HC),
Parkinson's
(PD),
multiple
sclerosis
(MS),
amyotrophic
lateral
(ALS),
temporal
epilepsy
(TLE),
depression,
schizophrenia
(SCZ).
Furthermore,
correlation
therapeutics
will
be
discussed.
Conversely,
mood
delivery,
exercise,
psychotropic
agents,
stress,
neurologic
drugs
can
influence
MGBA.
By
understanding
MGBA,
it
may
possible
to
facilitate
into
microbial-based
interventions
therapeutic
strategies
Nutrients,
Journal Year:
2025,
Volume and Issue:
17(2), P. 346 - 346
Published: Jan. 18, 2025
Background/objectives:
recent
studies
have
suggested
that
components
typical
of
the
Mediterranean
diet
(MedDiet)
are
associated
with
depression
and
anxiety
prevention.
In
this
sense,
main
objective
study
was
to
analyse
associations
between
adherence
MedDiet
symptoms
examine
whether
relationship
is
mediated
by
lean
mass
muscle
strength
index
(MSI).
Methods:
a
cross-sectional
(based
on
data
obtained
from
Nuts4Brain-Z
study)
conducted
2023–2024,
involving
428
university
students,
aged
18–30
years,
Spanish
public
university.
Depression
assessed
using
Beck
Inventory
(BDI-II),
via
General
Anxiety
Disorder-7
(GAD-7)
tool.
Adherence
MEDAS
questionnaire.
Lean
bioimpedance,
MSI
measured
dynamometer.
ANCOVA
models
were
used
test
mean
differences
in
scores
categories
(low
<
9
points
vs
high
≥
points).
Serial
multiple
mediation
models,
adjusted
for
confounders,
explore
role
relationships
symptoms.
Results:
students
exhibited
lower
(p
0.05)
than
did
low
MedDiet.
The
analysis
preliminarily
revealed
both
acted
as
mediators
anxiety.
Conclusion:adherence
per
se
does
not
appear
direct
effect
because
these
partially
(for
depression)
or
entirely
anxiety)
explained
MSI.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 22, 2025
Abstract
The
widespread
application
of
high-throughput
Next
Generation
Sequencing
(NGS)
technologies
has
made
microbiome
research
an
emerging
field
in
public
health
and
biomedical
sciences.
However,
there
are
still
many
challenges
that
need
to
be
addressed
this
field.
Pipelines
available
generate
data
across
cohorts
diverse,
sources
variation
recorded
evaluated
during
profiling
have
not
been
standardized.
Moreover,
meticulous
quality
control
the
processing,
from
collection
computational
quantification
is
challenging,
especially
large
population
studies.
Innovative
approaches
required
handle
samples
minimize
potential
bias
introduced
by
logistic
hurdles
biobanking.
In
paper,
we
describe
methodological
steps
surrounding
optimization
16s
rRNA
gut
two
prospective
R
Study
(mean
age
9.83
±
0.32
years)
Rotterdam
62.67
5.66
years).
This
paper
also
highlights
solutions
sample
mislabeling
large-scale
analysis.
To
summarize,
our
study
addresses
common
problems
human
research.
It
aims
improve
reliability
integrating
more
stringent
standards
into
