Mapping Biodiversity Coast‐to‐Coast‐to‐Coast Across Canada's Three Oceans Using eDNA Metabarcoding DOI Creative Commons
Loïc Jacquemot, Brian P. V. Hunt,

Shaorong Li

et al.

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(6)

Published: Nov. 1, 2024

ABSTRACT Marine biodiversity worldwide is rapidly declining, and nowhere this more evident than in coastal ecosystems where the impacts of climate change anthropogenic activities concentrate. The ongoing crisis affects all components marine food web, but data required to monitor shifts at continental scales are scarce taxonomically spatially heterogeneous. application environmental DNA metabarcoding can complement traditional approaches monitoring biodiversity, its efficiency detecting large‐scale biogeographic breaks remains be tested. Using 86 surface water samples collected during Canada C3 expedition summer 2017, we investigated metazoan across Canada's three oceans—North Pacific, Arctic North Atlantic—using multi‐marker eDNA metabarcoding. resulting dataset, combining information from seven separate amplicons, identified 1477 unique species ranging zooplankton mammals. We found that around separated into four clusters overlapped with known ecoregions, indicating a higher connectivity between Atlantic Pacific clusters. However, detection salmon Canadian suggests these may extending their distribution range poleward. By comparing occurrence recorded Ocean Biodiversity Information System (OBIS) for Alaska waters, 324 “unexpected” species. These results demonstrate importance primer selection species‐specific applications provide benchmark further work aimed validating identification map large spatial scale. Our showed powerful method an interoceanic Integrating programs valuable insights changes associated contribute filling gaps species‐at‐risk.

Language: Английский

Mapping Biodiversity Coast‐to‐Coast‐to‐Coast Across Canada's Three Oceans Using eDNA Metabarcoding DOI Creative Commons
Loïc Jacquemot, Brian P. V. Hunt,

Shaorong Li

et al.

Environmental DNA, Journal Year: 2024, Volume and Issue: 6(6)

Published: Nov. 1, 2024

ABSTRACT Marine biodiversity worldwide is rapidly declining, and nowhere this more evident than in coastal ecosystems where the impacts of climate change anthropogenic activities concentrate. The ongoing crisis affects all components marine food web, but data required to monitor shifts at continental scales are scarce taxonomically spatially heterogeneous. application environmental DNA metabarcoding can complement traditional approaches monitoring biodiversity, its efficiency detecting large‐scale biogeographic breaks remains be tested. Using 86 surface water samples collected during Canada C3 expedition summer 2017, we investigated metazoan across Canada's three oceans—North Pacific, Arctic North Atlantic—using multi‐marker eDNA metabarcoding. resulting dataset, combining information from seven separate amplicons, identified 1477 unique species ranging zooplankton mammals. We found that around separated into four clusters overlapped with known ecoregions, indicating a higher connectivity between Atlantic Pacific clusters. However, detection salmon Canadian suggests these may extending their distribution range poleward. By comparing occurrence recorded Ocean Biodiversity Information System (OBIS) for Alaska waters, 324 “unexpected” species. These results demonstrate importance primer selection species‐specific applications provide benchmark further work aimed validating identification map large spatial scale. Our showed powerful method an interoceanic Integrating programs valuable insights changes associated contribute filling gaps species‐at‐risk.

Language: Английский

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