Analytical Chemistry,
Journal Year:
2022,
Volume and Issue:
94(46), P. 16196 - 16203
Published: Nov. 11, 2022
Quantification
of
exosomal
multi-miRNA
can
reveal
the
initiation,
progression,
and
metastasis
tumors,
which
is
conducive
to
noninvasive
early
diagnosis
cancer.
However,
low-sensitivity
single-plex
detection
characteristics
traditional
methods
seriously
hinder
accuracy
specificity
miRNAs
in
cancer
diagnosis.
Herein,
we
design
an
ultramultiplexing
strategy
that
enables
simultaneous
sensitive
multiple
by
nanosatellites
(magnetic
beads
(MBs)
@
NaLnF4)
catalytic
hairpin
assembly
(CHA)
amplification
combination
with
inductively
coupled
plasma-mass
spectrometry
(ICP-MS)
diagnose
accurately.
The
competitive
binding
target
recognition
sequences
on
triggers
drop
NaLnF4
from
MBs,
followed
a
CHA
reaction
releases
more
labels
for
ICP-MS
detection.
This
method
used
detect
ten
types
simultaneously
limit
0.01
fM,
one
order
magnitude
lower
than
quantitative
reverse
transcription
polymerase
chain
(qRT-PCR)
method.
Linear
discriminant
analysis
as
machine
learning
algorithm
subsequently
applied
analyze
signals
multi-miRNA,
discrimination
cell
exosomes
reaches
98.6%.
In
clinical
cohort
42
patients,
including
five
healthy
controls,
achieves
accurate
classification
100%
accuracy.
Our
results
show
nanosatellites,
CHA,
provides
universal
biosensing
platform
ultrasensitive
targets.
Journal of Cellular and Molecular Medicine,
Journal Year:
2024,
Volume and Issue:
28(9)
Published: April 28, 2024
Abstract
Oestrogen
is
known
to
be
strongly
associated
with
ovarian
cancer.
There
was
much
work
show
the
importance
of
lncRNA
SNHG17
in
However,
no
study
has
revealed
molecular
regulatory
mechanism
and
functional
effects
between
oestrogen
development
metastasis
In
this
study,
we
found
that
expression
significantly
increased
cancer
positively
correlated
treatment.
could
promote
M2
macrophage
polarization
as
well
cells
SKOV3
ES2
cell
exosomal
expression.
When
exposure
oestrogen,
promoted
proliferation,
migration,
invasion
epithelial‐mesenchymal
transition
(EMT)
vitro,
tumour
growth
lung
vivo
by
accelerating
M2‐like
phenotype
macrophages.
Mechanically,
facilitate
release
CCL13
from
via
PI3K‐Akt
signalling
pathway.
Moreover,
CCL13‐CCR2
axis
identified
involved
behaviours
driven
oestrogen.
results
demonstrate
a
novel
exerts
an
oncogenic
effect
on
CCL13–CCR2–M2
upon
treatment,
which
may
potential
biomarker
therapeutic
target
for
responded
Frontiers in Oncology,
Journal Year:
2025,
Volume and Issue:
14
Published: Jan. 16, 2025
Oral
squamous
cell
carcinoma
(OSCC)
accounts
for
approximately
90%
of
all
oral
cancers,
significantly
impacting
the
survival
and
quality
life
patients.
Exosomes,
small
extracellular
vesicles
released
by
cells,
play
a
crucial
role
in
intercellular
communication
cancer.
Nevertheless,
their
function
mechanism
OSCC
remain
elusive.
Search
Pubmed,
Web
Science,
Cochrane
Library
using
keywords
OSCC,
exome,
diagnosis,
treatment
to
review
research
progress
exome
OSCC.
Based
on
these
results,
this
starting
from
biosynthesis,
structure,
contents
exosomes,
elaborates
exosomes
diagnosis
It
explores
potential
briefly
describes
challenges
researchers
currently
face.
Molecular Cancer,
Journal Year:
2025,
Volume and Issue:
24(1)
Published: Feb. 14, 2025
Extracellular
vesicles
(EVs),
nanoscale
secreted
by
cells,
have
attracted
considerable
attention
in
recent
years
due
to
their
role
tumor
immunomodulation.
These
facilitate
intercellular
communication
transporting
proteins,
nucleic
acids,
and
other
biologically
active
substances,
they
exhibit
a
dual
development
immune
evasion
mechanisms.
Specifically,
EVs
can
assist
cells
evading
surveillance
attack
impairing
cell
function
or
modulating
immunosuppressive
pathways,
thereby
promoting
progression
metastasis.
Conversely,
also
transport
release
immunomodulatory
factors
that
stimulate
the
activation
regulation
of
system,
enhancing
body's
capacity
combat
malignant
diseases.
This
functionality
presents
promising
avenues
targets
for
immunotherapy.
By
examining
biological
characteristics
influence
on
immunity,
novel
therapeutic
strategies
be
developed
improve
efficacy
relevance
cancer
treatment.
review
delineates
complex
immunomodulation
explores
potential
implications
approaches,
aiming
establish
theoretical
foundation
provide
practical
insights
advancement
future
EVs-based
immunotherapy
strategies.
The FASEB Journal,
Journal Year:
2025,
Volume and Issue:
39(6)
Published: March 13, 2025
Abstract
Breast
cancer
(BC)
is
one
of
the
most
common
malignant
tumors
among
women,
accounting
for
24.5%
all
cases
and
leading
to
15.5%
cancer‐related
mortality.
The
treatment
BC
patients
remains
a
significant
challenge
due
disease's
high
invasiveness,
elevated
metastatic
potential,
substantial
drug
resistance,
recurrence
rate.
Exosomes,
which
are
lipid‐bilayer
extracellular
vesicles
ranging
in
size
from
30
150
nm,
mediate
intercellular
communication
between
tumor
cells
surrounding
microenvironment
by
transferring
various
bioactive
substances,
such
as
proteins,
lipids,
nucleic
acids.
Recently,
growing
evidence
has
demonstrated
that
non‐coding
RNAs
(ncRNAs)
enriched
exosomes
play
critical
role
regulating
cell
proliferation,
metastasis,
angiogenesis
BC.
Consequently,
exosomal
ncRNAs
have
emerged
promising
therapeutic
target
treatment,
given
their
involvement
multiple
processes
progression.
This
review
provides
comprehensive
in‐depth
analysis
emerging
BC,
highlighting
potential
biological
mechanisms
advanced
applications
treatment.
Cancers,
Journal Year:
2022,
Volume and Issue:
14(9), P. 2317 - 2317
Published: May 7, 2022
Great
improvement
has
been
made
in
the
diagnosis
and
therapy
of
breast
cancer
patients.
However,
identification
biomarkers
for
early
diagnosis,
prognosis,
assessment
monitoring,
including
drug
resistance
detection
micro-metastases,
is
still
lacking.
Recently,
circulating
microRNAs
(miRNAs),
freely
blood
stream
or
entrapped
extracellular
vesicles
(EVs),
have
shown
to
a
potential
diagnostic,
prognostic
predictive
power.
In
this
review,
recent
findings
are
summarized,
both
at
preclinical
clinical
level,
related
miRNA
applicability
context
cancer.
Different
aspects,
technical
challenges,
discussed,
describing
potentialities
use
Even
though
more
methodological
standardized
studies
conducted
larger
selected
patient
cohorts
needed
support
effective
utility
as
biomarkers,
they
could
represent
novel
accessible
tools
be
transferred
into
practice.
International Journal of Molecular Sciences,
Journal Year:
2022,
Volume and Issue:
23(14), P. 7792 - 7792
Published: July 14, 2022
Breast
cancer
(BC)
is
a
major
public
health
problem,
with
key
pieces
of
information
needed
for
developing
preventive
and
curative
measures
still
missing.
For
example,
the
participation
chaperone
system
(CS)
in
carcinogenesis
anti-cancer
responses
poorly
understood,
although
it
can
be
predicted
to
crucial
factor
these
mechanisms.
The
chief
components
CS
are
molecular
chaperones,
here
we
discuss
four
them,
Hsp27,
Hsp60,
Hsp70,
Hsp90,
focusing
on
their
pro-carcinogenic
roles
BC
potential
anti-BC
therapies.
These
chaperones
targets
negative
chaperonotherapy,
namely
elimination/blocking/inhibition
chaperone(s)
functioning
favor
BC,
using,
instance,
Hsp
inhibitors.
also
employed
immunotherapy
against
as
adjuvants,
together
antigens.
Extracellular
vesicles
(EVs)
diagnosis
management
briefly
discussed,
considering
easily
accessible
carriers
biomarkers
shippers
agents
amenable
manipulation
controlled
delivery.
data
surveyed
from
many
laboratories
reveal
that,
enhance
understanding
role
BS
pathogenesis,
one
must
consider
physiological
system,
encompassing
diverse
members
throughout
body
interacting
ubiquitin–proteasome
chaperone-mediated
autophagy
machinery,
immune
(IS).
An
integrated
view
CS,
including
its
functional
partners
highly
dynamic
nature
EVs
transporting
reach
all
cell
compartments
which
they
needed,
opens
yet
unexplored
pathways
leading
that
interference
by
treatments
centered
components,
such
chaperones.
Frontiers in Immunology,
Journal Year:
2022,
Volume and Issue:
13
Published: May 11, 2022
The
prevalence
and
incidence
of
metabolic-associated
fatty
liver
disease
(MAFLD),
a
clinically
heterogeneous
whose
primary
clinical
therapies
include
dietary
control
exercise
therapy,
is
increasing
worldwide
constitutes
significant
medical
burden.
Gut
microbes
influence
the
physiopathological
processes
through
different
mechanisms
based
on
gut-liver
axis.
Exosomes
are
essential
carriers
intercellular
communication.
Most
previous
studies
have
focused
adipocyte-
hepatocyte-derived
exosomes,
while
critical
role
microbial-derived
exosomes
molecular
behind
them
in
MAFLD
received
little
attention.
Therefore,
we
searched
screened
latest
relevant
PubMeb
database
to
elucidate
link
between
pathogenesis
MAFLD,
mainly
terms
insulin
resistance,
intestinal
barrier,
inflammatory
response,
lipid
metabolism,
fibrosis.
aim
was
provide
theoretical
framework
support
for
protocols
innovative
drug
development.
Cell Death and Disease,
Journal Year:
2022,
Volume and Issue:
13(6)
Published: June 9, 2022
Abstract
Tumour
immunity
plays
an
important
role
in
the
development
of
cancer.
immunotherapy
is
component
antitumour
therapy.
Exosomes,
a
type
extracellular
vesicle,
act
as
mediators
intercellular
communication
and
molecular
transfer
play
essential
tumour
immunity.
Circular
RNAs
(circRNAs)
are
new
noncoding
RNA
that
enriched
within
exosomes.
In
this
review,
we
describe
effects
exosomal
circRNAs
on
various
immune
cells
mechanisms
these
effects,
including
macrophages,
neutrophils,
T
cells,
Natural
killer
(NK)
cells.
Next,
elaborate
latest
progress
exosome
extraction.
addition,
function
potential
prognostic
drug
sensitivity
marker
described.
We
present
great
promise
regulating
immunity,
predicting
patient
outcomes,
evaluating
efficacy.
