Capturing and releasing of hepatocellular carcinoma EpCAM+ and EpCAM- circulating tumor cells based on photosensitive intelligent nanoreactor DOI Creative Commons

Zhifang Mao,

Meng Hu, Qinglin Shen

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 30, 2024

Epithelial cell adhesion molecule negative circulating tumor cells (EpCAM- CTCs) and EpCAM positive CTCs (EpCAM + have different biological characteristics. Therefore, the isolation of EpCAM- is a new strategy to study heterogeneity cells. The azobenzene group (Azo) cyclodextrin (CD) composite system forms photosensitive molecular switch based on effect external light stimulation. We used technology specifically capturing using anti-EpCAM aptamers functionalized nanochips. Both can be connected Azo through 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) modification process. we assume that intelligent nanoreactor (PSINR) modified with capture CTCs; Utilizing characteristics aptamer ligand binding, PSINR Then, two PSINRs were separated stimulated release CTCs, respectively. Based expected reveal key mechanisms recurrence, metastasis drug resistance, make individualized treatment liver cancer more targeted, safe effective, provide basis for final realization accurate tumors.

Language: Английский

Electrochemiluminescence biosensor for specific detection of pancreatic ductal carcinoma through dual targeting of MUC1 and miRNA-196a DOI
Yuxuan Chen,

Zhuoxin Ye,

Mo Ma

et al.

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 254, P. 116241 - 116241

Published: March 23, 2024

Language: Английский

Citations

11
Enhancing detection and monitoring of circulating tumor cells: Integrative approaches in liquid biopsy advances DOI Creative Commons

Thanmayi Velpula,

Buddolla Viswanath

The Journal of Liquid Biopsy, Journal Year: 2025, Volume and Issue: 8, P. 100297 - 100297

Published: April 29, 2025

Language: Английский

Citations

0
Insights into the Precision Medicine of Pancreatic Cancer: Single-Cell Analysis DOI Open Access
Negar Jamshidi,

Nazanin Jamshidi,

Vahid Chaleshi

et al.

Journal of Chronic Diseases and Management, Journal Year: 2024, Volume and Issue: 8(1), P. 1 - 14

Published: Feb. 23, 2024

The rising incidence of pancreatic cancer (PC) is a global challenge paramount importance. Understanding the complex nature this disease crucial for addressing various issues within field, including early recurrence, metastasis, and formidable resistance to chemotherapy radiotherapy. complexity PC arises from diverse biological phenotypes present in tumor cells, creating mosaic-like diversity. lack comprehensive insights into functional aspects cellular molecular components further complicates treatment, leading suboptimal outcomes. To tackle these intricacies, researchers must explore foundations multifaceted disease. Profiling transcriptomes becomes powerful tool understanding subtle heterogeneity populations tissues. This approach allows deconstruction processes at individual cell level, revolutionizing our identity function. By carefully examining differences single-cell genomes, transcriptomes, epigenetic profiles among both primary metastatic, collected patient samples, we gain landscape heterogeneity. perspective has significant implications precision oncology context cancer. current study provides assessment empirical basis RNA sequencing technologies, methodologies isolation, conceptual framework integrating elements prognostic assessment, genomic accessibility, transcriptional dynamics, proteomic landscapes. combining components, aims establish coherent that facilitates development medicine approaches tailored unique challenges presented by

Language: Английский

Citations

0
Applications of spatial transcriptomics and artificial intelligence to develop integrated management of pancreatic cancer DOI

Rishabh Maurya,

Isha Chug,

Vignesh Vudatha

et al.

Advances in cancer research, Journal Year: 2024, Volume and Issue: unknown, P. 107 - 136

Published: Jan. 1, 2024

Language: Английский

Citations

0
Current biological implications and clinical relevance of metastatic circulating tumor cells DOI Creative Commons

Reza Shahhosseini,

Seyed Abbas Pakmehr,

Anis Elhami

et al.

Clinical and Experimental Medicine, Journal Year: 2024, Volume and Issue: 25(1)

Published: Nov. 15, 2024

Metastatic disease and cancer recurrence are the primary causes of cancer-related deaths. Circulating tumor cells (CTCs) disseminated (DTCs) driving forces behind spread cells. The emergence development liquid biopsy using rare CTCs as a minimally invasive strategy for early-stage detection improved management is promising advancement in recent years. However, before blood sample analysis clinical translation, precise isolation from patients' based on their biophysical properties, followed by molecular identification single-cell multi-omics technologies necessary to understand heterogeneity provide effective diagnosis monitoring progression. Additionally, understanding origin, morphological variation, interaction between metastatic niche, well regulatory immune cells, will offer new insights into CTC-based advanced targeting future trials.

Language: Английский

Citations

0
Electrochemiluminescence resonance energy transfer biosensor based on BNCs@Zn-PTC for ultrasensitive and low-potential detection of CA125 in pancreatic cancer diagnostics DOI
Yuxuan Chen,

Zhuoxin Ye,

Mo Ma

et al.

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: unknown, P. 137136 - 137136

Published: Dec. 1, 2024

Language: Английский

Citations

0
Mass Spectrometry-based Proteomics Technology in Pancreatic Cancer Research DOI Creative Commons
Xue Sun, Siyuan Wang, Catherine C. L. Wong

et al.

Journal of Pancreatology, Journal Year: 2023, Volume and Issue: 7(2), P. 145 - 163

Published: Nov. 14, 2023

Pancreatic ductal adenocarcinoma (PDAC) has become a significant health concern with increasing incidence and mortality rates over the past few decades. Researchers have turned their attention to cutting-edge mass spectrometry (MS) technology due its high-throughput accurate detection capacity, which plays vital role in understanding mechanisms discovering biomarkers for pancreatic diseases. In this review, we comprehensively investigate various methodologies of quantitative qualitative proteomics MS technologies, alongside bioinformatical platforms employed cancer research. The integration these optimized approaches provides novel insights into molecular underlying tumorigenesis disease progression, ultimately facilitating discovery potential diagnostic, prognostic biomarkers, therapeutic targets. robust MS-based strategy shows promise paving way early diagnosis personalized medicine patients.

Language: Английский

Citations

1
Capturing and releasing of hepatocellular carcinoma EpCAM+ and EpCAM- circulating tumor cells based on photosensitive intelligent nanoreactor DOI Creative Commons

Zhifang Mao,

Meng Hu, Qinglin Shen

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 12

Published: Aug. 30, 2024

Epithelial cell adhesion molecule negative circulating tumor cells (EpCAM- CTCs) and EpCAM positive CTCs (EpCAM + have different biological characteristics. Therefore, the isolation of EpCAM- is a new strategy to study heterogeneity cells. The azobenzene group (Azo) cyclodextrin (CD) composite system forms photosensitive molecular switch based on effect external light stimulation. We used technology specifically capturing using anti-EpCAM aptamers functionalized nanochips. Both can be connected Azo through 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) modification process. we assume that intelligent nanoreactor (PSINR) modified with capture CTCs; Utilizing characteristics aptamer ligand binding, PSINR Then, two PSINRs were separated stimulated release CTCs, respectively. Based expected reveal key mechanisms recurrence, metastasis drug resistance, make individualized treatment liver cancer more targeted, safe effective, provide basis for final realization accurate tumors.

Language: Английский

Citations

0