ER-mitochondria distance is a critical parameter for efficient mitochondrial Ca2+ uptake and oxidative metabolism

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: Oct. 10, 2024

IP

Language: Английский

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The Temperature of the First Cleavage Impacts Preimplantation Development and Newborn Viability

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3745 - 3745

Published: April 16, 2025

At the early developmental stage, embryos are susceptible to environmental factors, which modulate development trajectories. In our study, we examined how different incubation temperatures (35 °C, 37 and 39 °C) in vitro during first embryonic cleavage affect morphology, cell division rate, DNA methylation two-, four-, eight-cell viability of these two-cell transferred recipient females. Embryos kept at 35 °C for 24 h after fertilization two- four-cell showed enhanced variability size blastomeres 5mC level among blastomeres, as compared groups °C. This was associated with highest rate embryo death four- newborns. contrast, did not significantly impact dynamics but led a notably higher gestation failure other groups. The indicators group fell within an intermediate range. Therefore, conclude that decrease temperature zygotic genome activation (ZGA) highlights adaptive potential their initial cleavages, while increase does show clear effects on fate.

Language: Английский

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Decoding Cold Mechanisms of Enhanced Bone Repair through Sensory Receptors and Molecular Pathways

Published: Aug. 29, 2024

Applying cold to a bone injury can aid healing, though its mechanisms are complex. This study investigates how therapy impacts repair optimize healing. Cold was applied rodent model, with physiological responses analyzed. Vasoconstriction mediated by an increase in TRP channels, TRPA1 (p=0.012) and TRPM8 (p<0.001), within cortical defects enhancing sensory response blood flow regulation. exposure also elevated hypoxia (p < 0.01) VEGF expression 0.001), promoting angiogenesis vital for regeneration. Increased of osteogenic proteins PGC-1α = 0.039) RBM3 (p<0.008) suggests stimulated reparative processes. Enhanced osteoblast differentiation ALP presence at days 5 (three-fold, p=0.014) 10 (two-fold, p=0.010) were observed, along increased osteocalcin (OCN) day p 0.010) indicating mature osteoblasts capable mineralization. These findings highlight therapy's multifaceted effects on repair, offering insights therapeutic strategies.

Language: Английский

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The effect of temperature shock on enhancing sperm motility: A new insight into molecular and cellular mechanisms through current procedures

Medical Hypotheses, Journal Year: 2024, Volume and Issue: 188, P. 111377 - 111377

Published: May 11, 2024

Language: Английский

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Intermittent subdiffusion of short nuclear actin rods due to interactions with chromatin

Physical review. E, Journal Year: 2024, Volume and Issue: 110(1)

Published: July 18, 2024

The interior of cellular nuclei, the nucleoplasm, is a crowded fluid that pervaded by protein-decorated DNA polymers, chromatin. Due to complex architecture chromatin and multitude associated nonequilibrium processes, e.g., repair, nucleoplasm can be expected feature nontrivial material properties hence anomalous transport phenomena. Here, we have used single-particle tracking on nuclear actin rods probe such Our analysis reveals short in nucleus show an intermittent, antipersistent subdiffusion with clear signatures fractional Brownian motion. Moreover, diffusive motion heterogeneous intermittent switching trajectories between at least two different mobilities, most likely due transient associations In line this interpretation, hyperosmotic stress seen stall rods, whereas hypo-osmotic conditions yield reptationlike data highlights heterogeneity needs taken into account for understanding nucleoplasmic organization mechanobiology nuclei.

Language: Английский

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ER-mitochondria distance is a critical parameter for efficient mitochondrial Ca²⁺uptake and oxidative metabolism

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

ABSTRACT IP 3 receptor (IP R)-mediated Ca 2+ transfer at the mitochondria-endoplasmic reticulum (ER) contact sites (MERCS) drives mitochondrial uptake and oxidative metabolism is linked to different pathologies, including Parkinson’s disease (PD). The dependence of efficiency on ER-mitochondria distance remains unexplored. Employing molecular rulers that stabilize ER-mitochondrial distances 5 nm resolution, using genetically-encoded indicators targeting ER lumen sub-mitochondrial compartments, we now show a ∼20 optimal for due enrichment R MERCS. In human iPSC-derived astrocytes from PD patients, 20 MERCS were specifically reduced which correlated with reduction uptake. Our work determines precision flux between mitochondria suggests new paradigm fine control over function.

Language: Английский

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Decoding Cold Therapy Mechanisms of Enhanced Bone Repair through Sensory Receptors and Molecular Pathways

Biomedicines, Journal Year: 2024, Volume and Issue: 12(9), P. 2045 - 2045

Published: Sept. 9, 2024

Applying cold to a bone injury can aid healing, though its mechanisms are complex. This study investigates how therapy impacts repair optimize healing. Cold was applied rodent model, with the physiological responses analyzed. Vasoconstriction mediated by an increase in transient receptor protein channels (TRPs), potential ankyrin 1 (TRPA1;

Language: Английский

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