Protective Effects of Thymoquinone on Doxorubicin-induced Lipid Peroxidation and Antioxidant Enzyme Levels in Rat Peripheral Tissues DOI Creative Commons

Deniz Akpınar,

Tanju Mercan, Halit Demir

et al.

Pakistan Veterinary Journal, Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 1, 2023

We aimed to investigate the effects of thymoquinone (TQ) against Doxorubicin (DOX)-induced toxicity in liver, lung, kidney, testes, diaphragm and stomach tissues rats.Healthy twenty-eight Wistar albino male rats aged three months, were divided into four groups, each containing seven animals: Group 1: Control (C group); injected intraperitoneally with saline 5 times every other day given by gavage vehicle solution 2 (T group): Rats treated (10mg/kg gavage), 3 (D doxorubicin (3mg/kg) 4 (DT (injected 3mg/kg DOX) gavage).Group TQ (10mg/kg) was administered at 24-hour intervals for weeks.Saline (i.p.) day.Group The mg/kg DOX day.In addition, 24-h weeks.Group day.TQ weeks.At end experiment, animals euthanized sodium pentobarbital (50 mg/kg) their stomach, testicular removed measurement superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) malondialdehyde (MDA) levels.It observed that administration (in group D) increased lipid peroxidation decreased antioxidant enzyme levels peripheral rats.It determined treatment DT group) levels.In our study, it eliminated toxic protective revealed.

Language: Английский

Nanomaterials-based advanced systems for photothermal / photodynamic therapy of oral cancer DOI
Yue Wang, Lili Chang, Hongyu Gao

et al.

European Journal of Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 272, P. 116508 - 116508

Published: May 15, 2024

Language: Английский

Citations

20
Rnd3 protects against doxorubicin-induced cardiotoxicity through inhibition of PANoptosis in a Rock1/Drp1/mitochondrial fission-dependent manner DOI Creative Commons
Ge Wen, Xiaohua Zhang,

Jie Lin

et al.

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 4, 2025

Abstract Doxorubicin, a representative drug of the anthracycline class, is widely used in cancer treatment. However, Doxorubicin-induced cardiotoxicity (DIC) presents significant challenge its clinical application. Mitochondrial dysfunction plays central role DIC, primarily through disrupting mitochondrial dynamics. This study aimed to investigate impact Rnd3 (a Rho family GTPase 3) on with focus Cardiomyocyte-specific transgenic mice (Rnd3-Tg) and LSP/LSP (N-Tg) were established for vivo experiments, adenoviruses harboring (Ad-Rnd3) or negative control (Ad-Control) injected myocardium vitro experiments. The DIC model was using wild-type, N-Tg, Rnd3-Tg mice, subsequent intraperitoneal injection Dox 4 weeks. molecular mechanism explored RNA sequencing, immunofluorescence staining, co-immunoprecipitation assay, protein-protein docking. administration induced injury cardiac dysfunction, which ameliorated by overexpression. Further, augmentation expression mitigated fragmentation mediated dynamin-related protein 1 (Drp1), thereby ameliorating PANoptosis (pyroptosis, apoptosis, necroptosis) response Dox. Mechanically, interaction between Rho-associated kinase (Rock1) may impede Rock1-induced Drp1 phosphorylation at Ser616, thus inhibiting fission dysfunction. Interestingly, Rock1 knockdown nullified effects cardiomyocytes PANoptosis, as well Dox-induced remodeling elicited Rnd3. enhances resilience against stabilizing dynamics reducing PANoptosis. Our findings suggest that Rnd3/Rock1/Drp1 signaling pathway represents novel target mitigating modulating could be strategic approach safeguarding function patients undergoing

Language: Английский

Citations

2
Hyaluronic acid-based prodrug nanomedicines for enhanced tumor targeting and therapy: A review DOI
Renshuai Zhang,

Xiaohua Zhao,

Ang Jia

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 249, P. 125993 - 125993

Published: July 27, 2023

Language: Английский

Citations

30
Chloroquine and Chemotherapeutic Compounds in Experimental Cancer Treatment DOI Open Access
Natalia I. Agalakova

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(2), P. 945 - 945

Published: Jan. 12, 2024

Chloroquine (CQ) and its derivate hydroxychloroquine (HCQ), the compounds with recognized ability to suppress autophagy, have been tested in experimental works clinical trials as adjuvant therapy for treatment of tumors different origin increase efficacy cytotoxic agents. Such a strategy can be effective overcoming resistance cancer cells standard chemotherapy or anti-angiogenic therapy. This review presents results combined application CQ/HCQ conventional drugs (doxorubicin, paclitaxel, platinum-based compounds, gemcitabine, tyrosine kinases PI3K/Akt/mTOR inhibitors, other agents) malignancies obtained experiments on cultured cells, animal xenografts models, few trials. The effects such an approach viability tumor growth, well autophagy-dependent -independent molecular mechanisms underlying cellular responses CQ/HCQ, are summarized. Although majority vitro vivo studies shown that effectively sensitize agents potential chemotherapy, often inconsistent. Nevertheless, pharmacological suppression autophagy remains promising tool increasing development more specific inhibitors is required.

Language: Английский

Citations

13
Novel drug delivery systems: Insight into self-powered and nano-enabled drug delivery systems DOI Creative Commons
Ugochi E. Ewii, Adaeze Linda Onugwu,

Victor C. Nwokpor

et al.

Nano TransMed, Journal Year: 2024, Volume and Issue: 3, P. 100042 - 100042

Published: July 11, 2024

Novel drug delivery systems comprise sophisticated technology merged into systems. These are created with the aim of addressing limitations conventional For instance, inefficient in tackling challenging human diseases such as cancer. thus formed materials to enhance permeation target cells. They improve patients' compliance and efficacy. include self-powered microelectromechanical Nanotechnology is a rapidly growing promising field, especially system development disease therapy. often utilise nanocarriers due their numerous advantages. One benefits ability manipulate size surface functionalisation achieve site-specific targeting. Many researchers have reported controlled targeted release profiles. Nanocarriers propelled tissues, where entrapped drugs released, prolonging therapeutic efficacy decreasing undesired side effects. Hence, these offer delivery, low toxicity, high bioavailability improved efficiency. It essential understand mechanisms toxicity order develop efficient safe This review describes utilisation novel also dwells on loading, targeting from nanocarriers. The primary concerns clinical applications potential presented. expected that this will be helpful formulation seeking solutions cancer cardiovascular diseases.

Language: Английский

Citations

12
Clinical applications of STING agonists in cancer immunotherapy: current progress and future prospects DOI Creative Commons
Bin Wang, Wanpeng Yu, Hongfei Jiang

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 2, 2024

The STING (Stimulator of Interferon Genes) pathway is pivotal in activating innate immunity, making it a promising target for cancer immunotherapy. agonists have shown potential enhancing immune responses, particularly tumors resistant to traditional therapies. This scholarly review examines the diverse categories agonists, encompassing CDN analogues, non-CDN chemotypes, CDN-infused exosomes, engineered bacterial vectors, and hybrid structures small molecules-nucleic acids. We highlight their mechanisms, clinical trial progress, therapeutic outcomes. While these agents offer significant promise, challenges such as toxicity, tumor heterogeneity, delivery methods remain obstacles broader use. Ongoing research innovation are essential overcoming hurdles. could play transformative role treatment, patients with hard-to-treat malignancies, by harnessing body's system eliminate cells.

Language: Английский

Citations

11
Nanozyme-based synergistic therapeutic strategies against tumors DOI
Ye Zhang, Ning Zhang, Shouping Gong

et al.

Drug Discovery Today, Journal Year: 2025, Volume and Issue: unknown, P. 104292 - 104292

Published: Jan. 1, 2025

Language: Английский

Citations

1
Camptothecin-based prodrug nanomedicines for cancer therapy DOI
Renshuai Zhang, Jing Yu, Zhu Guo

et al.

Nanoscale, Journal Year: 2023, Volume and Issue: 15(44), P. 17658 - 17697

Published: Jan. 1, 2023

Camptothecin (CPT) is a cytotoxic alkaloid that attenuates the replication of cancer cells via blocking DNA topoisomerase 1.

Language: Английский

Citations

19
Strategies for the development of stimuli-responsive small molecule prodrugs for cancer treatment DOI Creative Commons

Yuxuan Tu,

Jianbao Gong,

Jing Mou

et al.

Frontiers in Pharmacology, Journal Year: 2024, Volume and Issue: 15

Published: July 31, 2024

Approved anticancer drugs typically face challenges due to their narrow therapeutic window, primarily because of high systemic toxicity and limited selectivity for tumors. Prodrugs are initially inactive drug molecules designed undergo specific chemical modifications. These modifications render the until they encounter conditions or biomarkers

Language: Английский

Citations

7
NOX4 aggravates doxorubicin-induced cardiomyocyte pyroptosis by increasing reactive oxygen species content and activating the NLRP3 inflammasome DOI Open Access
Hong Zeng,

Pengtao Zou,

Yanmei Chen

et al.

Cardiovascular Diagnosis and Therapy, Journal Year: 2024, Volume and Issue: 14(1), P. 84 - 100

Published: Feb. 1, 2024

Background: Nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4)-mediated reactive oxygen species (ROS) has been reported to induce cardiomyocyte apoptosis, but its effect on pyroptosis of cardiomyocytes rarely reported. This paper aimed explore the effects NOX4-mediated ROS production doxorubicin (DOX)-induced myocardial injury and through nucleotide-binding oligomerization domain-like receptor protein 3 (NLRP3) inflammasome. Methods: HL-1 cells were treated with DOX or mice (30 divided into five groups six mice/group) underwent intraperitoneal injection (5 mg/kg, once a week, times) injury, followed by assessment NOX4 NLRP3 expression in cell supernatant tissues. In cells, proliferation was tested MTT assay activity probes. The superoxide dismutase (SOD) activity, malondialdehyde (MDA) content, glutathione (GSH) evaluated kits. proteins assessed western blotting. Subsequently, altered determine pyroptosis. animal models utilized evaluate changes cardiac function using an echocardiographic system, these parameters measured including left ventricular ejection fraction (LVEF), fractional shortening (LVFS), end-diastolic diameter (LVEDD). Furthermore, content markers determined mice. Results: treatment led pyroptosis, as evidenced weakened LVEF, LVFS, (P<0.05), elevated LVEDD, ROS, MDA increased decreased SOD GSH (P<0.05). Additionally, highly-expressed (P<0.05) DOX-induced overexpression intensified levels aggravate which reversed scavenger N-acetyl-cysteine. it revealed that combination short hairpin RNA (sh)-NOX4 overexpressed (oe)-NLRP3 cardioprotective sh-NOX4 tissue vitro vivo. No died during experiments, only two ruled out due weight loss greater than 20%. Conclusions: activated inflammasome, thereby aggravating

Language: Английский

Citations

6