The Identification of Key Genes and Biological Pathways in Cardiac Arrest by Integrated Bioinformatics and Next Generation Sequencing Data Analysis DOI Open Access
Basavaraj Vastrad, Chanabasayya Vastrad

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

Abstract Cardiac arrest (CA) is a common cause of death world wide. The disease has lacks effective treatment. Efforts have been made to elucidate the molecular pathogenesis CA, but mechanisms remain elusive. To identify key genes and pathways in next generation sequencing (NGS) GSE200117 dataset was downloaded from Gene Expression Omnibus (GEO) database. DESeq2 tool used recognize differentially expressed (DEGs). ontology (GO) REACTOME pathway enrichment analyses were performed analyze DEGs associated signal g:Profiler IID database construct protein-protein interaction (PPI) network, modules analysis using Cytoscape. A miRNA-hub gene regulatory network TF-hub then constructed screen miRNAs, TFs hub by miRNet NetworkAnalyst Cityscape software. Receiver operating characteristic curve (ROC) verified genes. In total, 844 identified, comprising 414 up regulated 430 down GO indicated that for CA mainly enriched organonitrogen compound metabolic process, response stimulus, translation immune system. Ten (up-regulated: HSPA8, HOXA1, INCA1 TP53; down-regulated: HSPB1, LMNA, SNCA, ADAMTSL4 PDLIM7) screened. We also predicted miRNAs (hsa-mir-1914-5p hsa-mir-598-3p) (JUN PRRX2) targeting This study uses series bioinformatics technologies obtain hug genes, TFs, related CA. These results provide us with new ideas finding biomarkers treatment methods

Language: Английский

Mitochondrial Melatonin: Beneficial Effects in Protecting against Heart Failure DOI Creative Commons
Russel J. Reıter, Ramaswamy Sharma, Luiz Gustavo de Almeida Chuffa

et al.

Life, Journal Year: 2024, Volume and Issue: 14(1), P. 88 - 88

Published: Jan. 5, 2024

Cardiovascular disease is the cause of physical infirmity and thousands deaths annually. Typically, during heart failure, cardiomyocyte mitochondria falter in terms energy production metabolic processing. Additionally, inflammation accumulation non-contractile fibrous tissue contribute to cardiac malfunction. Melatonin, an endogenously produced molecule, experimentally reduces initiation progression atherosclerotic lesions, which are often basis coronary artery disease. The current review critically analyzes published data related experimental use melatonin forestall pathologies. Collectively, these studies document melatonin’s anti-atherosclerotic actions reducing LDL oxidation triglyceride levels, lowering endothelial malfunction, limiting adhesion molecule formation, preventing macrophage polarization M1 pro-inflammatory phenotype, changing cellular metabolism, scavenging destructive reactive oxygen species, proliferation invasion arterial smooth muscle cells into lesioned area, restricting ingrowth blood vessels from vasa vasorum, solidifying plaque cap reduce chance its rupture. Diabetic hyperglycemia, aggravates also inhibited by supplementation animals. potential value non-toxic as a possible inhibitor pathology humans should be seriously considered performing clinical trials using this multifunctional molecule.

Language: Английский

Citations

10
Melatonin ameliorates PM2.5-induced airway inflammation and apoptosis by PERK/eIF2α/ATF4/CHOP in chronic obstructive pulmonary disease mice DOI
Meng Shi, Kai Liu, Xin Li

et al.

Toxicology and Applied Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 117314 - 117314

Published: March 1, 2025

Language: Английский

Citations

1
Melatonin as a treatment for atherosclerosis: focus on programmed cell death, inflammation and oxidative stress DOI Creative Commons

Reza Asemi,

Elham Omidi Najafabadi,

Zahra Gholami Mahmoudian

et al.

Journal of Cardiothoracic Surgery, Journal Year: 2025, Volume and Issue: 20(1)

Published: April 12, 2025

Delaying the development of atherosclerosis (AS) and decreasing cardiac ischemia-reperfusion damage remain serious challenges for medical community. Chronic arterial disease, i.e., AS, is frequently linked to oxidative stress inflammation as significant contributing causes. AS risk factors, such hyperlipidemia, high blood pressure, age, hyperglycemia, smoking, cholesterol, irregular sleep patterns, can exacerbate in carotid artery further shrink its lumen. Finding new approaches that support plaque inhibition or stability an ongoing problem. The last ten years have shown us melatonin (MLT) affects cardiovascular system, although exact mechanisms action are yet unknown. MLT's direct free radical scavenger activity, indirect antioxidant qualities, anti-inflammatory capabilities all contribute atheroprotective effects on several pathogenic signaling pathways. Herein, we examine evidence showing MLT treatment has protective against AS-related diseases. numerous pieces puzzle been epigenetic biogenetic targets prevention therapy atherosclerotic processes identified.

Language: Английский

Citations

0
Protective effect of vitexin against high fat-induced vascular endothelial inflammation through inhibiting trimethylamineN-oxide-mediated RNA m6A modification DOI
Wenwen Li, Zeyuan Deng,

Shuang Xiao

et al.

Food & Function, Journal Year: 2024, Volume and Issue: 15(13), P. 6988 - 7002

Published: Jan. 1, 2024

A high-fat diet (HFD) is a major risk factor for cardiovascular disease.

Language: Английский

Citations

3
Melatonin and Vascular Function DOI Creative Commons
Leandro Mendes, Marcelo Queiroz, Cristina M. Sena

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(6), P. 747 - 747

Published: June 20, 2024

The indolamine hormone melatonin, also known as N-acetyl-5-methoxytrypamine, is frequently associated with circadian rhythm regulation. Light can suppress melatonin secretion, and photoperiod regulates levels by promoting its production secretion at night in response to darkness. This becoming more understood for functions an immune-modulatory, anti-inflammatory, antioxidant hormone. Melatonin may have a major effect on several diabetes-related disturbances, such hormonal imbalances, oxidative stress, sleep mood disorders, according recent research. has raised interest investigating the possible therapeutic advantages of treatment diabetic complications. In addition, studies described that been linked development diabetes, cancer, Alzheimer’s disease, immune system heart diseases. this review, we will highlight some regarding vascular biology.

Language: Английский

Citations

2
Melatonin stabilizes atherosclerotic plaques: an association that should be clinically exploited DOI Creative Commons
Russel J. Reıter, Ramaswamy Sharma, Alejandro Romero

et al.

Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11

Published: Dec. 11, 2024

Atherosclerosis is the underlying factor in premature death of millions humans annually. The cause often a result rupture an atherosclerotic plaque followed by discharge associated molecular debris into vessel lumen which occludes artery leading to ischemia downstream tissue and morbidity or mortality individual. This most serious when it occurs heart (heart attack) brain (stroke). Atherosclerotic plaques are classified as either soft, rupture-prone, hard, resistant. Melatonin, production diminishes with age, has major actions converting soft hard plaques. Experimentally, melatonin reduces ingrowth capillaries from tunica media relieving pressure on plaque, reducing intraplaque hemorrhage limiting size necrotic core. Moreover, promotes formation collagen invading vascular smooth muscle cells strengthen crown making resistant rupture. Melatonin also powerful antioxidant anti-inflammatory agent such that oxidative damage tissues limits inflammation both contribute cap weakness. Additional benefits relative atherosclerosis inhibition adhesion molecules endothelial cell surface, invasion monocytes arterial intima, conversion M2 macrophages pro-inflammatory M1 macrophages. Given high physiological financial cost cardiac neural ischemic events, this information should be given priority clinical setting.

Language: Английский

Citations

2
The Identification of Key Genes and Biological Pathways in Cardiac Arrest by Integrated Bioinformatics and Next Generation Sequencing Data Analysis DOI Open Access
Basavaraj Vastrad, Chanabasayya Vastrad

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 19, 2024

Abstract Cardiac arrest (CA) is a common cause of death world wide. The disease has lacks effective treatment. Efforts have been made to elucidate the molecular pathogenesis CA, but mechanisms remain elusive. To identify key genes and pathways in next generation sequencing (NGS) GSE200117 dataset was downloaded from Gene Expression Omnibus (GEO) database. DESeq2 tool used recognize differentially expressed (DEGs). ontology (GO) REACTOME pathway enrichment analyses were performed analyze DEGs associated signal g:Profiler IID database construct protein-protein interaction (PPI) network, modules analysis using Cytoscape. A miRNA-hub gene regulatory network TF-hub then constructed screen miRNAs, TFs hub by miRNet NetworkAnalyst Cityscape software. Receiver operating characteristic curve (ROC) verified genes. In total, 844 identified, comprising 414 up regulated 430 down GO indicated that for CA mainly enriched organonitrogen compound metabolic process, response stimulus, translation immune system. Ten (up-regulated: HSPA8, HOXA1, INCA1 TP53; down-regulated: HSPB1, LMNA, SNCA, ADAMTSL4 PDLIM7) screened. We also predicted miRNAs (hsa-mir-1914-5p hsa-mir-598-3p) (JUN PRRX2) targeting This study uses series bioinformatics technologies obtain hug genes, TFs, related CA. These results provide us with new ideas finding biomarkers treatment methods

Language: Английский

Citations

0