Calreticulin—From the Endoplasmic Reticulum to the Plasma Membrane—Adventures of a Wandering Protein

Cancers, Journal Year: 2025, Volume and Issue: 17(2), P. 288 - 288

Published: Jan. 17, 2025

Calreticulin (CRT) is a 46 kDa highly conserved protein initially identified as calregulin, prominent Ca2+-binding of the endoplasmic reticulum (ER). Subsequent studies have established that CRT functions in ER's folding response and Ca2+ homeostatic mechanisms. An ER retention signal on carboxyl terminus suggested was restricted to ER. However, identification nucleus cytosol has multi-compartmental, multifunctional protein. also plays an important role cancer progression. Most recently, cell surface shown be potent 'eat-me' key uptake apoptotic viable cells by phagocytes. Elevated exposure outer leaflet been linked with anticancer immunity superior therapeutic outcomes patients non-small lung carcinoma, colorectal acute myeloid leukemia, ovarian cancer, high-grade serous carcinomas. Mutations gene subset myeloproliferative neoplasms. The most recent from our laboratory revealed new significant function for extracellular plasminogen receptor. This discovery profound implications understanding neoplasms, specifically, essential thrombocythemia.

Language: Английский

Mass Spectrometry‐Based Proteomics Technologies to Define Endogenous Protein‐Protein Interactions and Their Applications to Cancer and Viral Infectious Diseases

Mass Spectrometry Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

An intricate network of protein assemblies and protein-protein interactions (PPIs) underlies nearly every biological process in living systems. The organization these cellular networks is highly dynamic intimately tied to the genomic proteomic landscapes a cell. Disruptions normal PPIs can impair functions contribute development human diseases. In recent years, targeting has emerged as an attractive strategy for drug discovery. Consequently, identification characterization endogenous PPIs-those occurring naturally under physiological conditions-has become crucial unraveling molecular mechanisms driving pathology laying groundwork novel diagnostics therapeutics. Owing numerous technological advancements, mass spectrometry (MS)-based proteomics transformed study at systems-level. This review focuses on approaches that enable physiologically relevant interactions, spanning complex-centric structure-centric analyses. Additionally, their applications define native contexts cancer viral infectious diseases highlighted.

Language: Английский

A photosensitive modification of recombinant Salmonella carrying shRNA −PD-L1 promotes host immunity against poorly immunogenic tumors

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 157034 - 157034

Published: Oct. 1, 2024

Language: Английский