Sustainable artificial coral reef restoration using nanoclays and composite hydrogel microcapsules

RSC Sustainability, Journal Year: 2024, Volume and Issue: 2(3), P. 616 - 620

Published: Jan. 1, 2024

Nanoclays, nanocomposite hydrogel microcapsules, and encapsulated bacteria can be considered as cement replacements for artificial coral reef development.

Language: Английский

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A modular plasmid toolkit applied in marine bacteria reveals functional insights during bacteria-stimulated metamorphosis

mBio, Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 2, 2023

A conspicuous roadblock to studying marine bacteria for fundamental research and biotechnology is a lack of modular synthetic biology tools their genetic manipulation. Here, we applied, generated new parts for, plasmid toolkit study in the context symbioses host-microbe interactions. To demonstrate utility this system, genetically manipulated bacterium Pseudoalteromonas luteoviolacea, which stimulates metamorphosis model tubeworm, Hydroides elegans. Using these tools, quantified constitutive native promoter expression, developed reporter strains that enable imaging host-bacteria interactions, used CRISPR interference (CRISPRi) knock down secondary metabolite host-associated gene. We broader system testing tractability are known be associated with diverse symbioses. These efforts resulted successful conjugation 12 from Alphaproteobacteria Gammaproteobacteria classes. Altogether, present demonstrates how strategies investigation microbes potential implications environmental restoration biotechnology. IMPORTANCE Marine Proteobacteria attractive targets engineering due ability produce diversity bioactive metabolites involvement Modular cloning toolkits have become standard microbes, such as Escherichia coli, because they innumerable mix-and-match DNA assembly options. However, not yet been applied most bacterial species. In work, adapt use set by association its host animal This work provides proof concept can address basic science questions innovations.

Language: Английский

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A modular plasmid toolkit applied in marine Proteobacteria reveals functional insights during bacteria-stimulated metamorphosis

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Jan. 31, 2023

A conspicuous roadblock to studying marine bacteria for fundamental research and biotechnology is a lack of modular synthetic biology tools their genetic manipulation. Here, we applied, generated new parts for, plasmid toolkit study in the context symbioses host-microbe interactions. To demonstrate utility this system, genetically manipulated bacterium Pseudoalteromonas luteoviolacea , which stimulates metamorphosis model tubeworm, Hydroides elegans . Using these tools, quantified constitutive native promoter expression, developed reporter strains that enable imaging host-bacteria interactions, used CRISPR interference (CRISPRi) knock down secondary metabolite host-associated gene. We broader system rapidly creating iteratively testing tractability by modifying are known be associated with diverse symbioses. These efforts enabled successful transformation twelve across two Proteobacteria classes, four orders ten genera. Altogether, present demonstrates how strategies investigation microbes implications environmental restoration biotechnology.

Language: Английский

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Bacterial microbiome variation across symbiotic states and clonal lines in a cnidarian model

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 10

Published: Feb. 10, 2023

Introduction Exaiptasia diaphana is a popular model organism for exploring the symbiotic relationship observed between cnidarians and their microsymbionts. While physiological roles of algal photosymbionts (Symbiodinaceae) are well studied, contributions bacterial communities less defined in this system. Methods We investigated microbial variation distinct parts body state across four genets held identical environmental conditions using 16s rRNA gene amplicon sequencing. Results found differentially abundant taxa part that highlight these bacteria may play holobiont heterotrophy nutrient cycling. Beta-diversity analysis revealed states consistent with previous studies; however, we did not observe presence previously reported core microbiota. also community differences clonal lines, despite years rearing conditions. Conclusion These findings suggest microbiome greatly influenced by host genetics unpredictable influences.

Language: Английский

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Microbiome-assisted restoration of degraded marine habitats: a new nature-based solution?

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 10

Published: Sept. 20, 2023

Microorganisms interact with all biological components in a variety of ways. They contribute to increase the efficiency marine food webs and facilitate adaptation multicellular organisms climate change other human-induced impacts. Increasing evidence suggests that microbiomes are essential for health species, maintaining productive ecosystems, thus sustainable functioning global biosphere. Marine typically species- or habitat-specific susceptible environmental human-driven changes. The microbiota seagrasses, macroalgae, mangroves tropical corals benefits their hosts by increasing fitness, contributing removal toxic compounds, conferring protection against pathogens, and/or supporting nutrient requirements. Alterations might have negative consequences on species’ health, survival, overall ecosystem functioning. Despite key ecological role potential restoration degraded habitats is still largely unexplored. Here we present literature survey existing information associated habitat-forming species suggest resilience/recovery damaged can depend changes microbiota. Nature-based solutions relying microbiome analyses (also through omics approaches) enable monitoring transplanted organisms/metacommunities identification/production probiotics/bio-promoters stabilize unhealthy conditions transplants. In context international strategies concerning restoration, use scientific knowledge acquired deserves be exploited assist both traditional innovative approaches. success habitat may our ability maintain, along restored habitats, functional

Language: Английский

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Editorial: Interaction between marine invertebrates and symbiotic microbes in a changing environment: Community structure and ecological functions

Frontiers in Marine Science, Journal Year: 2023, Volume and Issue: 9

Published: Jan. 12, 2023

EDITORIAL article Front. Mar. Sci., 12 January 2023Sec. Microbial Symbioses Volume 9 - 2022 | https://doi.org/10.3389/fmars.2022.1128906

Language: Английский

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Help Me, Symbionts, You’re My Only Hope: Approaches to Accelerate Our Understanding of Coral Holobiont Interactions

Published: Sept. 14, 2022

Tropical corals construct the three-dimensional framework for one of most diverse ecosystems on planet, providing habitat to a plethora species across taxa. However, these ecosystem engineers are facing unprecedented challenges, such as increasing disease prevalence and marine heatwaves associated with anthropogenic global change. As result, major declines in coral cover health being observed world's oceans, often due breakdown coral-associated symbioses. Here, we review interactions between symbiotic partners holobiont – cnidarian host, algae family Symbiodiniaceae, microbiome that influence trait variation, including molecular mechanisms underlie symbiosis resulting physiological benefits different microbial partnerships. In doing so, highlight current formation maintenance cnidarian-Symbiodiniaceae symbiosis, role immunity pathways play this relationship. We emphasize understanding complex is challenging when you consider vast genetic variation host algal symbiont, well their highly microbiome, which also an important player health. Given among partners, propose several research directions approaches focused model systems emerging technologies will broaden our how partner may facilitate prediction phenotype, especially under rapid environmental

Language: Английский

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Coral black band disease in Indonesia: An overview

The Egyptian Journal of Aquatic Research, Journal Year: 2024, Volume and Issue: 50(1), P. 103 - 109

Published: March 1, 2024

Coral reefs stand out as exceptionally diverse ecosystems globally, serving crucial roles nurseries and nutrient sources for marine life. Beyond their significance ecosystems, these play a vital role in shielding humans from erosion by acting barriers against waves storms. Unfortunately, the existence of coral faces threat black band disease, particularly Indonesia. This disease can lead to rapid decline reefs, characterized microbial mat presence containing filamentous cyanobacteria consortium, resulting tissue loss, lesions, mortality. The limited awareness this Indonesia compounds issue. In review, we aim present comprehensive information on covering its definition, historical context, cases Indonesia, potential contributing factors, drawing secondary sources. There are 29 documented scattered across Sumatra, Java, Sulawesi, NTT, Papua, with Acropora Montipora being most frequently affected genera. Given relatively silent nature cases, it is imperative bring greater attention Microbiome engineering emerges suggested treatment. literature review serves enhance biodiversity

Language: Английский

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Microbial Nitrogen Removal in Reef-building Corals: a Light-sensitive Process

Chemosphere, Journal Year: 2024, Volume and Issue: 359, P. 142394 - 142394

Published: May 20, 2024

Language: Английский

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Extinction Chains Reveal Intermediate Phases Between the Safety and Collapse in Mutualistic Ecosystems

Engineering, Journal Year: 2024, Volume and Issue: unknown

Published: July 1, 2024

Ecosystems are undergoing unprecedented persistent deterioration due to unsustainable anthropogenic human activities, such as overfishing and deforestation, the effects of damage on ecological stability uncertain. Despite recent advances in experimental theoretical studies regime shifts tipping points, tools for understanding extinction chain, which is sequence species extinctions resulting from overexploitation, still lacking, especially large-scale nonlinear networked systems. In this study, we developed a mathematical tool predict chains ecosystems under multiple exploitation situations verified it 26 real-world mutualistic networks various sizes densities. We discovered five phases during process: safe, partial extinction, bistable, tristable, collapse, enabled optimal design restoration strategies degraded or collapsed validated our approach using 20-year dataset an eelgrass project. Counterintuitively, also found specific region diagram spanning rates competition intensities, where exploiting more helps increase biodiversity. Our computational provides insights into harvesting, fishing, exploitation, deforestation plans while conserving restoring biodiversity ecosystems.

Language: Английский

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