Triazole fungicides disrupt embryonic stem cell differentiation: Potential modulatory role of the retinoic acid signaling pathway

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 283, P. 116859 - 116859

Published: Aug. 12, 2024

The developmental toxicity and human health risks of triazole fungicides (TFs) have attracted worldwide attention due to the ability enter body in a variety ways. Nevertheless, specific mechanism by which TFs exert remains incompletely understood. Given that retinoic acid (RA) signaling pathway are closely related development, this study aimed screen identify developmentally disabled chemicals commonly used reveal potential effects on retardation through RA mouse embryonic stem cells (mESCs). Specifically, six typical (myclobutanil, tebuconazole, hexaconazole, propiconazole, difenoconazole, flusilazole) were exposed construction an embryoid bodies (EBs)-based vitro global differentiation models. Our results clarified various disturbed lineage commitment during early development. Crucially, activation pathway, alters expression key genes interferes transport metabolism retinol, may be responsible for effect. Furthermore, molecular docking, dynamics simulations, experiments using receptor α inhibitor provide evidence supporting modulatory role injury. current offers new insights into involved interfere with process mESCs, is crucial understanding impact pregnancy

Language: Английский

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Maternal Gut Microbiome-Mediated Epigenetic Modifications in Cognitive Development and Impairments: A New Frontier for Therapeutic Innovation

Nutrients, Journal Year: 2024, Volume and Issue: 16(24), P. 4355 - 4355

Published: Dec. 17, 2024

Cognitive impairment in various mental illnesses, particularly neuropsychiatric disorders, has adverse functional and clinical consequences. While genetic mutations epigenetic dysregulations of several genes during embryonic adult periods are linked to cognitive the composition diversity resident bacteria gastrointestinal tract—shaped by environmental factors—also influence brain epigenome, affecting behavior functions. Accordingly, many recent studies have provided evidence that human gut microbiota may offer a potential avenue for improving deficits. In this review, we provide an overview relationship between impairment, alterations microbiome, periods. We examine how factors beyond genetics—such as lifestyle, age, maternal diet—impact composition, diversity, functionality consequently influencing performance. Additionally, explore microbiome signatures biomarkers predicting risk older adults. This article also explores roles nutritional deficiencies programming disorders perinatal period offspring, well promise microbiome-targeted therapeutics with effects prevent or alleviate dysfunctions infants, middle-aged adults, Unsolved challenges mitigating translation into practice discussed, lastly.

Language: Английский

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The Fungicide Ipconazole Can Activate Mediators of Cellular Damage in Rat Brain Regions

Toxics, Journal Year: 2024, Volume and Issue: 12(9), P. 638 - 638

Published: Aug. 31, 2024

This study aimed to investigate the toxicity of fungicide ipconazole on oxidative status, cell death and inflammasome complex activation in hypothalamus, cerebral cortex, striatum hippocampus rats. Female albino rats were randomly divided into a control group four groups treated with at doses 1, 5, 10 20 mg/kg b.w., administered for six days. Ipconazole significantly increased MDA ROS levels all brain regions studied, while reducing catalase enzyme activity. The molecular expression death-related genes (AKT1, APAF1, BNIP3, CASP3 BAX) (CASP1, IL1β, IL6, NLRP3, NFĸB TNFα) was also assessed, showing least one region. findings demonstrate that induces central nervous system mammals, highlighting its potential role as risk factor development neurodegenerative disorders individuals exposed this contaminant.

Language: Английский

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Environmental chemical-induced adverse effects on gut microbiota and their implications for the etiopathogenesis of chronic neurological diseases

Advances in neurotoxicology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 81

Published: Jan. 1, 2024

Language: Английский

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Artemether Restores the Barrier Damage and Intestinal Dysbiosis to Alleviate Postoperative Cognitive Dysfunction Via Inhibiting Tlr4/Myd88/Traf6 Pathway

Published: Jan. 1, 2024

Objective : To study the effect of Artemether (ART) on postoperative cognitive dysfunction (POCD) rats and its barrier damage intestinal dysbiosis mediated by TLR4/MYD88/TRAF6 signaling pathway.Method: We simulated pathological process POCD in mice with an exploratory laparotomy animal model. A total 30 C57BL/6 were randomly divided into three groups: 10 for CON group, POCD+ART (40mg/kg daily) group . Behavioral tests used to examine function mice. 16S rRNA sequencing was employed detect gut microbiota changes. Alcian blue staining applied observe integrity. Two-photon imaging permeability blood-brain barrier. The molecules pathway alternations tested QPCR western blot. Immunofluorescence activation glial cells expression LPS hippocampus.Results: Our findings indicated that anesthesia surgery could impair spatial learning memory capacity aged mouse feces revealed significantly changed composition diversity microbiota, resulting destruction homeostasis increased production endotoxins. Meanwhile, integrity interfered surgery. Further, we artemether as a therapeutic agent, which maintained microbiome homeostasis, restored synaptic function, reversed restores alleviate via inhibiting pathway.

Language: Английский

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Ameliorative effects of gallic acid on tebuconazole–induced adverse effects in the cerebellum of adult albino rats: histopathological and immunohistochemical evidence

Ultrastructural Pathology, Journal Year: 2024, Volume and Issue: 48(5), P. 351 - 366

Published: Aug. 6, 2024

Tebuconazole (TEB) is a common triazole sterol demethylation inhibitor fungicide utilized to manage variety of diseases in crops like cereals, fruits, and vegetables. The aim this work was assess the effects TEB on structure cerebellum adult albino rats possible protective impact co-administration Gallic acid (GA). Four groups forty male were randomly selected, group I received corn oil through daily gavage for 4 weeks. Group II GA dissolved normal saline at dose 100 mg/kg weeks, III administered with its acceptable intake (0.02 body weight) IV both GA. For light microscopic, ultrastructural, immunohistochemical investigations, cerebellar specimens prepared. exposure led neuronal damage form degenerated Purkinje cells vacuolated cytoplasm, areas lost cells, basket appeared neuropil, granule clumped congested between them, dilated islands, weak positive bcl2 immunoreactions numerous GFAP-positive astrocytes. mitigated TEB-mediated histological changes cortex. We concluded that caused neurons rat cortex degenerate undergo apoptosis. had neuroprotective benefit against toxicity

Language: Английский

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Recombinant protein LPxT-GYLEQ attenuates cognitive impairment by ameliorating oxidative stress in D-galactose-induced aging mice model

Food Bioscience, Journal Year: 2024, Volume and Issue: 62, P. 105079 - 105079

Published: Sept. 8, 2024

Language: Английский

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Research on the Mechanism of Buyang Huanwu Decoction in the Amelioration of Age-Associated Memory Impairment Based on the “Co-occurrence Network Regulation of Intestinal Microecology-Host Metabolism-Immune Function”

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 337, P. 118819 - 118819

Published: Sept. 19, 2024

Language: Английский

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An update of the impact of pesticide exposure on memory and learning

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1)

Published: Nov. 11, 2024

The dependence on the use of pesticides in agribusiness leads to an increase human exposure these chemical compounds through occupational activity, contamination environment, food and water. nervous system is target most insecticidal when are poorly selective, they can harm non-target species, including humans. This work aimed investigate effects pesticide memory learning. Articles published PubMed database between 2015 2024 were reviewed. One hundred sixty-one articles included this review, which one thirty two preclinical studies twenty-nine studies. Among studies, organophosphates, pyrethroids, neonicotinoids, carbamates, addition herbicides, fungicides mixed exposures was associated with learning deficits animals such as bees, mosquitoes, fishes, mice, rats, bats. Olfactory, associative aversive learning, well capacity affected by pesticides. Most evaluated environmental organophosphate pesticides, pyrethroids carbamates outcomes interest. Living close application areas having parents working agriculture worse scores childhood adolescence. Prenatal insecticides has already been poorer attention skills attention-deficit/hyperactivity disorder (ADHD) symptoms, intellectual disability, autism spectrum disorder, motor delays, problems. In adults, during activity also performance. evidence found review suggests that different mechanisms action able cause some cognitive impairment. Developmental exposure, chronic contribute performance, especially memory.

Language: Английский

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Enhanced Quasi-Meshing Hotspot Effect Integrated Embedded Attention Residual Network for Culture-Free SERS Accurate Determination of Fusarium Spores

Biosensors and Bioelectronics, Journal Year: 2024, Volume and Issue: 271, P. 117053 - 117053

Published: Dec. 9, 2024

Language: Английский

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