Integrons in the development of antimicrobial resistance: critical review and perspectives

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 31, 2023

Antibiotic resistance development and pathogen cross-dissemination are both considered essential risks to human health on a worldwide scale. Antimicrobial genes (AMRs) acquired, expressed, disseminated, traded mainly through integrons, the key players capable of transferring from bacterial chromosomes plasmids their integration by integrase target pathogenic host. Moreover, integrons play central role in disseminating assembling connected with antibiotic commensal species. They exhibit large concealed diversity natural environment, raising concerns about potential for comprehensive application adaptation. should be viewed as dangerous pool determinants “One Health approach.” Among three documented classes reported viz., class-1, 2, 3, class 1 has been found frequently associated AMRs humans is critical genetic element serve therapeutics gene silencing or combinatorial therapies. The direct method screening cassettes linked pathogenesis harbored novel way assess health. In last decade, they have witnessed surveying integron-associated increased drug tolerance rising pathogenicity microbes. Consequently, we aimed unravel structure functions mechanism understanding horizontal transfer one trophic group another. Many updates related extensively explored. Additionally, an updated account assessment prevailing grossly detailed—lastly, estimation AMR dissemination employing biomarkers also highlighted. current review will pave clinical devising roadmap solution pathogenicity. Graphical Abstract graphical abstract displays how integron-aided humans: Transposons capture integron yield high mobility that res sites plasmids. These plasmids, turn, promote acquired into diverse acquisitions resistant transferred transfer.

Language: Английский

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Anthropogenic pollution drives the bacterial resistome in a complex freshwater ecosystem

Chemosphere, Journal Year: 2023, Volume and Issue: 331, P. 138800 - 138800

Published: April 28, 2023

Aquatic ecosystems in anthropogenically impacted areas are important reservoirs of antibiotic resistance genes (ARGs) allochthonous origin. However, the dynamics different ARGs within bacterial communities lakes and rivers, as well factors that drive their selection, not completely understood. In this study, we analysed fate resistome (total content metal genes, MRGs) for a period six months (summer-winter) continuum lake-river-lake system (Lake Varese, River Bardello, Lake Maggiore) Northern Italy, by shotgun metagenomics. The metagenomic data were then compared with chemical, physical microbiological data, to infer role anthropogenic pressure sampling stations. MRGs more abundant diverse characterised highest pollution. date influenced MRGs, higher abundances summer (August) than fall or winter, when impact treated wastewater discharge river was limited water flow from Varese. ARG MRG significantly correlated they co-occurred main network analysis modules potential pathogenic bacteria. Different levels selectively promoted specific while others, generally waters, affected pressure. Reducing level resulted rapid decrease most ARGs. From our results, promoting spread resistances pathogens aquatic ecosystem becomes clear. Finally highlight strict correlation between suggesting co-selection stressed communities.

Language: Английский

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Conversion of swine manure into biochar for soil amendment: Efficacy and underlying mechanism of dissipating antibiotic resistance genes

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 871, P. 162046 - 162046

Published: Feb. 8, 2023

Language: Английский

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Deciphering the dynamics and driving mechanisms of high-risk antibiotic resistome in size-fractionated bacterial community during drinking water chlorination via metagenomic analysis

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 466, P. 133572 - 133572

Published: Jan. 24, 2024

Language: Английский

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Roles and fates of antibiotics in anaerobic digestion of waste activated sludge: Insights to pro- and re-duction of antibiotic resistance genes

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 500, P. 156633 - 156633

Published: Oct. 11, 2024

Language: Английский

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Integrons in the Age of Antibiotic Resistance: Evolution, Mechanisms, and Environmental Implications: A Review

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2579 - 2579

Published: Dec. 13, 2024

Integrons, which are genetic components commonly found in bacteria, possess the remarkable capacity to capture gene cassettes, incorporate them into their structure, and thereby contribute an increase genomic complexity phenotypic diversity. This adaptive mechanism allows integrons play a significant role acquiring, expressing, spreading antibiotic resistance genes modern age. To assess current challenges posed by integrons, it is necessary have thorough understanding of characteristics. review aims elucidate structure evolutionary history highlighting how use antibiotics has led preferential selection various environments. Additionally, explores involvement dissemination across diverse settings, while considering potential transmission factors routes. delves arrangement cassettes within ability rearrange, mechanisms governing expression, process excision. Furthermore, this study examines presence clinically relevant wide range environmental sources, shedding light on anthropogenic influences propagation environment.

Language: Английский

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Horizontal gene transfer in plant microbiomes: integrons as hotspots for cross-species gene exchange

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 29, 2024

Plant microbiomes play important roles in plant health and fitness. Bacterial horizontal gene transfer (HGT) can influence outcomes, driving the spread of both growth-promoting phytopathogenic traits. However, community dynamics, including range genetic elements bacteria involved this process are still poorly understood. Integrons recently shown to be abundant microbiomes, associated with HGT across broad phylogenetic boundaries. They facilitate cassettes, small mobile that collectively confer a diverse suite adaptive functions. Here, we analysed 5,565 plant-associated bacterial genomes investigate prevalence functional diversity integrons niche. We found particularly Pseudomonadales, Burkholderiales, Xanthomonadales. In total, detected nearly 9,000 many could growth promotion or phytopathogenicity, suggesting might role mutualistic pathogenic lifestyles. The rhizosphere was enriched cassettes transport metabolism substrates, they may aid adaptation environment, which is rich root exudates. also cross-species HGT, enhanced phyllosphere. This finding provide an ideal opportunity promote by fostering genes relevant leaf health. Together, our findings suggest drive have potential host adaptation.

Language: Английский

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High rate of multidrug resistance and integrons in Escherichia coli isolates from diseased ducks in select regions of China

Poultry Science, Journal Year: 2023, Volume and Issue: 102(10), P. 102956 - 102956

Published: July 25, 2023

With the increasing number of ducks being raised and consumed, it is crucial to monitor presence multidrug resistant (MDR) bacteria in duck farming. Waterfowl, such as ducks, can contribute rapid dissemination antibiotic resistance genes (ARGs). The objective this study was investigate antimicrobial (AMR), ARGs, mobile genetic elements (MGEs), IS26, tbrC, ISEcp1 Escherichia coli(E. coli) isolated from intestinal contents diseased between 2021 2022 Sichuan, Chongqing Anhui, China. AMR phenotypes 201 E. coli strains were determined using minimum inhibitory concentrations (MICs) method. Subsequently, polymerase chain reaction sequencing techniques employed screen for integron-integrase (intI1, intI2, intI3 genes), gene cassettes (GCs), MGEs, ARGs. results demonstrated that 96.5% isolates at least 1 antibiotic, with 88.1% displaying MDR phenotype. highest phenotype observed trimethoprim-sulfamethoxazole (88.1%). Furthermore, class 2 integrons detected 68.2% 3.0% all isolates, respectively, whereas no 3 found. Ten types GCs identified variable regions integrons. Moreover, 10 MGEs 46 combinations, IS26 exhibiting detection rate (89.6%). Among 22 tetA (77.1%) most frequently detected. In conjugational transfer experiment, transconjugants found carry specific ARGs their MIC values significantly higher than those recipient J53, indicating status bacteria. This emphasizes necessity monitoring farms. It provides valuable insights into complex formation mechanisms may aid preventing controlling spread waterfowl breeding farm.

Language: Английский

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Emerging threat to antibiotic resistance: Impact of mycotoxin deoxynivalenol on gut microbiota and clonal expansion of extensively drug-resistant enterococci

Environment International, Journal Year: 2025, Volume and Issue: 197, P. 109353 - 109353

Published: Feb. 28, 2025

Language: Английский

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Occurrence and fate of CECs (OMPs, ARGs and pathogens) during decentralised treatment of black water and grey water

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 915, P. 169863 - 169863

Published: Jan. 6, 2024

Decentralised wastewater treatment is becoming a suitable strategy to reduce cost and environmental impact. In this research, the performance of two technologies treating black water (BW) grey (GW) fractions urban sewage carried out in decentralised produced three office buildings. An Anaerobic Membrane Bioreactor (AnMBR) BW Hybrid preanoxic (H-MBR) containing small plastic carrier elements, GW were operated at pilot scale. Their potential on reducing release contaminants emerging concern (CECs) such as Organic Micropollutants (OMPs), Antibiotic Resistance Genes (ARGs) pathogens was studied. After 226 d operation, stable operation achieved both systems: AnMBR removed 92.4 ± 2.5 % influent COD, H-MBR 89.7 3.5 %. Regarding OMPs, profile compounds differed between GW, being matrix with more detected higher concentrations (up μg L−1). For example, case ibuprofen 23.63 3.97 L−1, 3 orders magnitude than those GW. The most abundant ARGs sulfonamide resistant genes (sul1) integron class 1 (intl1) Pathogenic bacteria counts reduced log units AnMBR. Bacterial loads much lower BW, no bacterial re-growth observed for effluents after H-MBR. None selected enteric viruses line.

Language: Английский

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