Comparative Endpoint Sensitivity of Bioanalytical Tools for Glucocorticoid Receptor Agonism Surveillance in Aquatic Matrices DOI
Alexander R. Cole, Bryan W. Brooks

ACS ES&T Water, Journal Year: 2023, Volume and Issue: 3(9), P. 3082 - 3092

Published: Aug. 25, 2023

Synthetic glucocorticoids, which occur globally in wastewater effluent discharges, surface waters, and reuse projects, are endocrine disruptors contaminants of emerging concern (CECs) relevance for water quality surveillance. Because diverse bioanalytical tools increasingly employed as new approach methodologies vitro to vivo extrapolations assessments, including glucocorticoid receptor (GR) agonist activity, we examined endpoint sensitivities multiple common methods. We probabilistic environmental hazard assessments initially examine the likelihood each assay identifying GR hazards discharges waters. then used chemical toxicity distributions advance an understanding relative sensitivity among assays by these CECs elicit agonistic response at environmentally relevant concentrations. observed commercially available other reported refereed literature generally be more sensitive than any included ToxCast efforts. Our observations emphasize importance further studies inform selection, use, interpretation responses within during extrapolation detection activity assessments.

Language: Английский

Seasonal variability and risk evaluation of emerging organic contaminants in European river: linking in silico and in vitro approaches to prioritize hazardous EOCs DOI
Göran Klobučar, Ana Selak,

Draženka Stipaničev

et al.

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 120840 - 120840

Published: Jan. 1, 2025

Language: Английский

Citations

1
What, How, When, and Where: Spatiotemporal Water Quality Hazards of Cyanotoxins in Subtropical Eutrophic Reservoirs DOI
Kevin M. Stroski, Daniel L. Roelke, Crista M. Kieley

et al.

Environmental Science & Technology, Journal Year: 2024, Volume and Issue: 58(3), P. 1473 - 1483

Published: Jan. 11, 2024

Though toxins produced during harmful blooms of cyanobacteria present diverse risks to public health and the environment, surface water quality surveillance cyanobacterial is inconsistent, spatiotemporally limited, routinely relies on ELISA kits estimate total microcystins (MCs) in waters. Here, we employed liquid chromatography tandem mass spectrometry examine common cyanotoxins, including five microcystins, three anatoxins, nodularin, cylindrospermopsin, saxitoxin 20 subtropical reservoirs spatially distributed across a pronounced annual rainfall gradient. Probabilistic environmental hazard analyses identified whether values for cyanotoxins were exceeded if these exceedances varied spatiotemporally. MC-LR was most congener detected, but it not consistently observed with other toxins, MC-YR, which detected at highest concentrations spring many observations above California human recreation guideline (800 ng/L). Cylindrospermopsin also quantitated 40% eutrophic reservoirs; detections did exceed US Environmental Protection Agency swimming/advisory level (15,000 Our have implications routine monitoring practices, traditionally use MC levels often limit collection samples summer months near reservoir impoundments, further indicate that spatiotemporal efforts are necessary understand when occur throughout year.

Language: Английский

Citations

5
Comparative Glucocorticoid Receptor Agonism: In Silico, In Vitro, and In Vivo and Identification of Potential Biomarkers for Synthetic Glucocorticoid Exposure DOI Creative Commons
Alexander R. Cole, Brett R. Blackwell, Jenna E. Cavallin

et al.

Environmental Toxicology and Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Abstract The glucocorticoid receptor (GR) is present in almost every vertebrate cell and utilized many biological processes. Despite an abundance of mammalian data, the structural conservation cross-species susceptibility, particularly for aquatic species, has not been well defined. Efforts to reduce, refine, and/or replace animal testing have increased, driving impetus advance development new approach methodologies (NAMs). Here we used silico, vitro, vivo methods elucidate a greater understanding receptor-mediated effects synthetic exposure teleost fish. Evolutionary amino acid residues critical transcriptional activation was confirmed silico using sequence alignment predict across species susceptibility. Subsequent vitro assays zebrafish human GR provided evidence physiological congruence agonism. Finally, adult fathead minnows (Pimephales promelas) were exposed glucocorticoids, dexamethasone (0.04, 400, 4,000 µg/L) beclomethasone dipropionate (130 µg/L), agonism via digital polymerase chain reaction; addition, EcoToxChip analyses identified potential mRNA biomarkers following exposure. These findings support use NAMs potentially reduce multispecies experimentation while providing empirical that expands taxonomic domain applicability molecular initiating event within broader adverse outcome pathway network.

Language: Английский

Citations

0
Glucocorticoids as emerging pollutants in surface water: A systematic review on their global occurrence and distribution DOI
Maria Cristina Trajano da Silva, R. L. da Silva,

Tiago Salles Teixeira

et al.

Environmental Research, Journal Year: 2025, Volume and Issue: 273, P. 121280 - 121280

Published: March 1, 2025

Language: Английский

Citations

0
RP-HPLC method development and validation for the quantification of prednisolone and salbutamol with their simultaneous removal from water using modified clay–activated carbon adsorbents DOI Creative Commons

M. Ramadan Mahmoud,

Samar M. Mahgoub,

Rania Abdelazeem

et al.

RSC Advances, Journal Year: 2025, Volume and Issue: 15(11), P. 8675 - 8695

Published: Jan. 1, 2025

Salbutamol sulfate (SAL) and prednisolone (PRD) are commonly used for treating respiratory inflammatory conditions, yet they frequently detected in aquatic ecosystems, posing significant risks to life biodiversity. Despite the growing concern over pharmaceutical pollution, there is a lack of reliable sustainable methods quantifying these drugs both environmental samples, as well effective adsorbents their removal from contaminated water. This study aims fill this gap by developing reversed-phase high-performance liquid chromatography (RP-HPLC) method SAL PRD, while also creating an organoclay-activated carbon composite adsorbent removing The HPLC was validated linearity, precision, accuracy, robustness, specificity, with detection limits 1.06 μg mL-1 0.95 PRD. demonstrated high efficiency drugs, achieving maximum adsorption capacities 731.64 mg g-1 888.75 PRD at pH 7, dose 0.4 g temperature 45 °C. Thermodynamic analysis revealed that process endothermic spontaneous. Characterization using FTIR, SEM, XRD, BET confirmed its structure. Adsorption followed Langmuir model Sips equilibrium reached within 240 minutes following pseudo-second-order kinetics. Ethanol proved more than acetone acetic acid desorbing SAL, found be cost-effective, offering practical solution large-scale water treatment. Sustainability assessments ComplexGAPI, BAGI, RGB 12 algorithms highlighted strong friendliness. research provides valuable insights quality control remediation pollutants.

Language: Английский

Citations

0
Exposure to synthetic steroid hormones and precocious puberty in girls: A case-control study DOI Creative Commons
Jingyi Tang, Yao Chen, Peng Xue

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 283, P. 116814 - 116814

Published: July 30, 2024

Synthetic steroid hormones are an emerging class of environmental pollutants, but their influence on pubertal timing remains unclear. This case-control study explored the association between synthetic hormone exposure and precocious puberty. Using ultrahigh performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), were detected in urine samples from 229 Chinese girls, aged 6-9 years. Puberty status was assessed using Tanner staging by professional pediatricians. We conducted least absolute shrinkage selection operator (LASSO) regression combined with logistic regression. Besides, we evaluated joint effects mixture identified main contributor Weighted quantile sum (WQS) model Bayesian kernel machine (BKMR) model. The reflected inverse individual puberty halcinonide [OR (95 %CI): 0.20 (0.07, 0.46)], budesonide 0.77 (0.62, 0.95)]. In utilizing WQS model, showed a marginal mixture, not significant 0.88 (0.75, 1.04)]. Prednisolone (0.31), fluorometholone acetate (0.24), dexamethasone (0.12) had highest weight. Consistently, associated BKMR conclusion, exposure, among girls. It highlighted management residual environment provided direction for prevention

Language: Английский

Citations

1
Exposure effects of synthetic glucocorticoid drugs on skeletal developmental and immune cell function in zebrafish DOI Creative Commons
Charles M. Hamilton, Matthew J. Winter, Jonathan S. Ball

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 955, P. 176781 - 176781

Published: Oct. 10, 2024

Synthetic glucocorticoids (GCs) are used to treat a wide range of human health conditions and as such frequently detected in the aquatic environment. This, together with highly conserved nature glucocorticoid system across vertebrates means that potential for biological effects GCs fish is relatively high. Here, we found exposure zebrafish (Danio rerio) environmentally relevant concentrations 4 most widely synthetic (beclomethasone dipropionate, budesonide, fluticasone propionate, prednisolone), from 0 days post fertilisation (dpf), resulted no on embryo-larval development or bone cartilage formation. However, after equivalents therapeutic plasma levels, developmental abnormalities were observed included pericardial oedema, blood pooling alterations jaw cartilage. Furthermore, using double transgenic osteoblast chondrocyte reporter line, up 10 dpf lower all compounds at, above, concentrations. In case beclomethasone reduction intercranial distance was at concentration 0.1 μg/L. Using further lines fluorescently tagged neutrophils macrophages, also show embryo-larvae (0-4 dpf) tested altered immune cell migration, but only high Collectively, our findings GC impacts development, function, skeletal formation, predominantly greater than those currently reported Despite this, however, it suggested studies longer times, mixtures multiple (many act via same mechanism action) warranted before can confidently assert these commonly contaminants do not pose risk wild.

Language: Английский

Citations

1
Dose-, stage- and sex- difference of prenatal prednisone exposure on placental morphological and functional development DOI
Xiaoming Zha,

Man Fang,

Wen Zhong

et al.

Toxicology Letters, Journal Year: 2024, Volume and Issue: 402, P. 68 - 80

Published: Nov. 22, 2024

Language: Английский

Citations

1
Prenatal prednisone exposure disturbs fetal kidney development and its characteristics DOI Creative Commons
Zhiping Xia,

Songdi Wang,

Wen Wang

et al.

Journal of Environmental Sciences, Journal Year: 2023, Volume and Issue: 145, P. 75 - 87

Published: Oct. 14, 2023

Prednisone is a synthetic glucocorticoid that commonly used in both human and veterinary medication. Now, it also recognized as an emerging environmental contaminant. Pregnant women may be exposed to prednisone actively or passively through multiple pathways cause developmental toxicity the fetus. However, impact of prenatal exposure (PPE) on fetal kidney development remains unclear. In this study, pregnant mice were administered intragastrically during full-term pregnancy with different doses (0.25, 0.5, 1 mg/(kg·day)), at dose mg/(kg·day) gestational days (GD) (GD0-9, GD10-18, GD0-18). The euthanized GD18. HE staining revealed dysplasia, enlarged glomerular Bowman's capsule space reduced capillary network PPE groups. expression podocyte mesangial cell marker genes was significantly overall gene renal tubules collecting ducts markedly increased. All above effects more pronounced high-dose, pregnancy, female fetuses. Studies mechanism have Six2, increased Hnf1β, Hnf4α, Wnt9b, inhibited glial line-derived neurotrophic factor (GDNF) Notch signaling pathways. conclusion, study demonstrated there sex difference kidney, time effect manifested > early mid-late pregnancy.

Language: Английский

Citations

3
Pollution characteristics and ecological risk assessment of glucocorticoids in the Jiangsu section of the Yangtze River Basin DOI

Lichao Tan,

Keke Xu,

Shengxin Zhang

et al.

Frontiers of Environmental Science & Engineering, Journal Year: 2024, Volume and Issue: 18(11)

Published: Sept. 13, 2024

Language: Английский

Citations

0