Decay of enveloped SARS-CoV-2 and non-enveloped PMMoV RNA in raw sewage from university dormitories DOI Creative Commons
Li Ye, Kurt T. Ash, Dominique C. Joyner

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: April 28, 2023

Introduction Although severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA has been frequently detected in sewage from many university dormitories to inform public health decisions during the COVID-19 pandemic, a clear understanding of SARS-CoV-2 persistence site-specific raw is still lacking. To investigate persistence, field trial was conducted University Tennessee sewage, similar municipal wastewater. Methods The decay enveloped and non-enveloped Pepper mild mottle virus (PMMoV) investigated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) at 4°C 20°C. Results Temperature, followed concentration level RNA, most significant factors that influenced first-order rate constants ( k ) RNA. mean values were 0.094 day −1 0.261 At high-, medium-, low-concentration levels 0.367, 0.169, 0.091 , respectively. Furthermore, there statistical difference between PMMoV different temperature conditions. Discussion first rates for both temperatures statistically comparable which showed sensitivity elevated but not This study provides evidence viral conditions levels.

Language: Английский

Performance evaluation of virus concentration methods for implementing SARS-CoV-2 wastewater based epidemiology emphasizing quick data turnaround DOI Creative Commons
Md Ariful Islam Juel, Nicholas Stark,

Bridgette Nicolosi

et al.

The Science of The Total Environment, Journal Year: 2021, Volume and Issue: 801, P. 149656 - 149656

Published: Aug. 14, 2021

Wastewater based epidemiology (WBE) has drawn significant attention as an early warning tool to detect and predict the trajectory of COVID-19 cases in a community, conjunction with public health data. This means monitoring for outbreaks been used at municipal wastewater treatment centers analyze trends entire communities, well by universities other community living environments monitor spread buildings. Sample concentration is crucial, especially when viral abundance raw below threshold detection RT-qPCR analysis. We evaluated performance rapid ultrafiltration-based virus method using InnovaPrep Concentrating Pipette (CP) Select compared this established electronegative membrane filtration (EMF) method. sensitivity SARS-CoV-2 quantification, surrogate recovery rate, sample processing time. Results suggest that CP concentrator more efficient concentrating from EMF About 25% samples tested negative concentrated produced positive signal protocol. Increased control confirms observation. optimized protocol adding AVL lysis buffer sonication, increase virus. Sonication increased Bovine Coronavirus (BCoV) 19%, which seems compensate loss during centrifugation. Filtration time decreases approximately 30% protocol, making optimal choice building surveillance applications where quick turnaround necessary.

Language: Английский

Citations

51
Wastewater surveillance of SARS-CoV-2 in dormitories as a part of comprehensive university campus COVID-19 monitoring DOI
Emily Lu,

Yuehan Ai,

Angela Davis

et al.

Environmental Research, Journal Year: 2022, Volume and Issue: 212, P. 113580 - 113580

Published: June 6, 2022

Language: Английский

Citations

33
Monitoring SARS-CoV-2 in wastewater during New York City's second wave of COVID-19: sewershed-level trends and relationships to publicly available clinical testing data DOI Creative Commons
Catherine Hoar,

Francoise Chauvin,

Alexander Clare

et al.

Environmental Science Water Research & Technology, Journal Year: 2022, Volume and Issue: 8(5), P. 1021 - 1035

Published: Jan. 1, 2022

SARS-CoV-2 viral loads in New York City were significantly correlated with clinical case rates corresponding sewersheds.

Language: Английский

Citations

32
Predicting COVID-19 cases using SARS-CoV-2 RNA in air, surface swab and wastewater samples DOI Creative Commons
Helena M. Solo‐Gabriele,

Shelja Kumar,

Samantha Abelson

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 857, P. 159188 - 159188

Published: Oct. 4, 2022

Language: Английский

Citations

29
Decay of enveloped SARS-CoV-2 and non-enveloped PMMoV RNA in raw sewage from university dormitories DOI Creative Commons
Li Ye, Kurt T. Ash, Dominique C. Joyner

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: April 28, 2023

Introduction Although severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) RNA has been frequently detected in sewage from many university dormitories to inform public health decisions during the COVID-19 pandemic, a clear understanding of SARS-CoV-2 persistence site-specific raw is still lacking. To investigate persistence, field trial was conducted University Tennessee sewage, similar municipal wastewater. Methods The decay enveloped and non-enveloped Pepper mild mottle virus (PMMoV) investigated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) at 4°C 20°C. Results Temperature, followed concentration level RNA, most significant factors that influenced first-order rate constants ( k ) RNA. mean values were 0.094 day −1 0.261 At high-, medium-, low-concentration levels 0.367, 0.169, 0.091 , respectively. Furthermore, there statistical difference between PMMoV different temperature conditions. Discussion first rates for both temperatures statistically comparable which showed sensitivity elevated but not This study provides evidence viral conditions levels.

Language: Английский

Citations

17