Inhibition
of
nitrite
oxidizing
bacteria
(NOB)
is
important
for
efficient
wastewater
treatment.
Here,
we
demonstrate
a
novel
strategy
based
on
the
detachment
and
reattachment
biofilm
to
establish
partial
nitrification
anammox
in
membrane
aerated
reactor
(MABR).
The
results
showed
that
using
free
ammonia
as
sole
inhibitor
start
up
took
more
time,
NOB
acclimated
inhibition.
Detaching
made
it
possible
contact
deeper
zones
while
at
same
time
washing
out
reactor.
Nitrite
accumulation
efficiency
(NAE)
increased
70%
50
days
when
both
high
loading
were
applied.
With
short
hydraulic
retention
concentrations
nitrite,
activity
appeared
resulted
nitrogen
removal
60%.
Microbial
community
analysis
indicated
Nitrosomonas,
Nitrospira,
Candidatus
Brocadia
main
functional
taxa.
Anaerobic
(AnAOB)
proliferated
within
biofilm,
inhibiting
growth
by
competing
nitrite.
Endogenous
denitrifying
also
grew,
coexisting
with
AnAOB.
Predicted
abundance
genes
related
fermentation
correlated
those
involved
denitrification,
indicating
possibility
heterotrophic
denitrification.
These
findings
help
further
our
understanding
control-based
strategies
management
have
implications
establishing
autotrophic
MABRs.
Environmental Science & Technology,
Journal Year:
2024,
Volume and Issue:
58(28), P. 12532 - 12541
Published: June 28, 2024
While
partial
nitrification
(PN)
has
the
potential
to
reduce
energy
for
aeration,
it
proven
be
unstable
when
treating
low-strength
wastewater.
This
study
introduces
an
innovative
combined
strategy
incorporating
a
low
rate
of
oxygen
supply,
pH
control,
and
sulfide
addition
selectively
inhibit
nitrite-oxidizing
bacteria
(NOB).
led
stable
PN
in
laboratory-scale
membrane
aerated
biofilm
reactor
(MABR).
Over
period
260
days,
nitrite
accumulation
ratio
exceeded
60%
synthetic
sewage
containing
50
mg
NH
Frontiers of Environmental Science & Engineering,
Journal Year:
2024,
Volume and Issue:
18(7)
Published: March 25, 2024
Abstract
Wastewater
treatment
plants
are
the
major
energy
consumers
and
significant
sources
of
greenhouse
gas
emissions,
among
which
biological
nitrogen
removal
wastewater
is
an
important
contributor
to
carbon
emissions.
However,
traditional
heterotrophic
denitrification
still
has
problems
excessive
residual
sludge
requirement
external
sources.
Consequently,
development
innovative
low-carbon
nitrate
technologies
necessary.
This
review
outlines
key
roles
sulfur
autotrophic
hydrogen
in
treatment.
The
discovered
nitrate/nitrite
dependent
anaerobic
methane
oxidation
enables
sustainable
emission
reduction
by
utilizing
available
situ
.
Photosynthetic
microorganisms
exhibited
a
promising
potential
achieve
carbon-negative
removal.
Specifically,
algal-bacterial
symbiosis
system
photogranules
offer
effective
prospective
options
for
Then,
emerging
technology
photoelectrotrophic
underlying
photoelectron
transfer
mechanisms
discussed.
Finally,
we
summarize
prospect
these
technologies,
highlighting
that
solar-driven
area
future
guiding
significance
design
systems.
ISME Communications,
Journal Year:
2024,
Volume and Issue:
4(1)
Published: Jan. 1, 2024
Abstract
Methane
(CH4),
an
important
greenhouse
gas,
significantly
impacts
the
local
and
global
climate.
Our
study
focused
on
composition
activity
of
methanotrophs
residing
in
lakes
Tibetan
Plateau,
a
hotspot
for
climate
change
research.
Based
field
survey,
family
Methylomonadaceae
had
much
higher
relative
abundance
freshwater
than
brackish
saline
lakes,
accounting
~92%
total
aerobic
methanotrophs.
Using
microcosm
sediment
incubation
with
13CH4
followed
by
high
throughput
sequencing
metagenomic
analysis,
we
further
demonstrated
that
was
actively
oxidizing
CH4.
Moreover,
various
methylotrophs,
such
as
genera
Methylotenera
Methylophilus,
were
detected
13C-labeled
DNAs,
which
suggested
their
participation
CH4-carbon
sequential
assimilation.
The
presence
CH4
metabolism,
tetrahydromethanopterin
ribulose
monophosphate
pathways,
identified
metagenome-assembled
genomes
Methylomonadaceae.
Furthermore,
they
potential
to
adapt
oxygen-deficient
conditions
utilize
multiple
electron
acceptors,
metal
oxides
(Fe3+),
nitrate,
nitrite,
survival
Tibet
lakes.
findings
highlighted
predominance
associated
microbes
active
consumers,
potentially
regulating
emissions
These
insights
contributed
understanding
plateau
carbon
cycle
emphasized
significance
mitigating
change.
mSystems,
Journal Year:
2024,
Volume and Issue:
9(10)
Published: Sept. 24, 2024
ABSTRACT
Bioflocs
are
microbial
aggregates
that
play
a
pivotal
role
in
shaping
animal
health,
gut
microbiota,
and
water
quality
biofloc
technology
(BFT)-based
aquaculture
systems.
Despite
the
worldwide
application
of
BFT
industries,
our
comprehension
community
composition
functional
potential
floc-associated
microbiota
(FAB
community;
≥3
µm
size
fractions)
remains
rudimentary.
Here,
we
utilized
genome-centric
metagenomic
approach
to
investigate
FAB
shrimp
systems,
resulting
reconstruction
520
metagenome-assembled
genomes
(MAGs)
spanning
both
bacterial
archaeal
domains.
Taxonomic
analysis
identified
Pseudomonadota
Bacteroidota
as
core
members,
with
approximately
93%
recovered
MAGs
unclassified
at
species
level,
indicating
large
uncharacterized
phylogenetic
diversity
hidden
community.
Functional
annotation
these
unveiled
their
complex
carbohydrate-degrading
involvement
carbon,
nitrogen,
sulfur
metabolisms.
Specifically,
genomic
evidence
supported
ammonium
assimilation,
autotrophic
nitrification,
denitrification,
dissimilatory
nitrate
reduction
ammonia,
thiosulfate
oxidation,
sulfide
oxidation
pathways,
suggesting
community’s
versatility
for
aerobic
anaerobic
Conversely,
genes
associated
heterotrophic
assimilatory
reduction,
sulfate
were
undetected.
Members
Rhodobacteraceae
emerged
most
abundant
metabolically
versatile
taxa
this
intriguing
Our
compendium
is
expected
expand
available
genome
collection
from
such
underexplored
environments.
By
elucidating
structure
metabolic
capabilities,
study
provides
valuable
insights
into
key
biogeochemical
processes
occurring
aquacultures
major
contributors
driving
processes.
IMPORTANCE
Biofloc
has
sustainable
approach,
utilizing
(bioflocs)
improve
health.
However,
specific
within
responsible
benefits
largely
unknown.
Compounding
challenge,
many
resist
laboratory
cultivation,
hindering
taxonomic
analyses.
To
address
gaps,
employed
binning
recover
over
500
systems
operating
South
Korea
China.
Through
analyses,
deciphered
gene
content
diverse
taxa,
shedding
light
on
roles
like
nitrogen
Notably,
findings
underscore
taxa-specific
contributions
microbes
environments,
particularly
carbon
degradation
removal
toxic
substances
nitrate,
sulfide.
