CRISPR/Cas9 mediated targeted knock-in of eglA gene to improve endoglucanase activity of Aspergillus fumigatus LMB-35Aa DOI Creative Commons

J. S. Benites-Pariente,

Ilanit Samolski, Yvette Ludeña

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 23, 2024

Bioeconomy goals for using biomass feedstock biofuels and bio-based production has arisen the demand fungal strains enzymes processing. Despite well-known Trichoderma Aspergillus commercial strains, continuous bioprospecting revealed biodiversity potential of degrading enzymes. The strain fumigatus LMB-35Aa a great as source lignocellulose-degrading Nevertheless, genetic improvement should be considered to increase its biotechnological potential. Molecular manipulation based on homologous direct recombination (HDR) in filamentous fungi poses challenge since low rate. Currently, CRISPR/Cas9-mediated mutagenesis can enable precise efficient editing genomes. In this study, gene strategy improving endoglucanase activity A. was successfully used, which constitutes first report heterologous cellulase technology. For this, eglA from niger ATCC 10,864 integrated into conidial melanin pksP locus, facilitated selection edited events discerned by emergence albino colonies. Heterologous EglA enzyme biofilm fermentation system resulted 40% mutant compared wild type.

Language: Английский

Fermentation of sorghum with Aspergillus strains: A promising and sustainable pathway to enzyme production- comprehensive review DOI Creative Commons
Pratul Dipta Somadder, Antoine P. Trzcinski, Guangnan Chen

et al.

Renewable and Sustainable Energy Reviews, Journal Year: 2025, Volume and Issue: 213, P. 115456 - 115456

Published: Feb. 9, 2025

Language: Английский

Citations

1
Alginate Oligosaccharides Alleviate Salt Stress in Rice Seedlings by Regulating Cell Wall Metabolism to Maintain Cell Wall Structure and Improve Lodging Resistance DOI Creative Commons
Youwei Du,

Huimin Zhao,

Naijie Feng

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(9), P. 1215 - 1215

Published: April 28, 2024

Salt stress is one of the major abiotic stresses that damage structure and composition cell walls. Alginate oligosaccharides (AOS) have been advocated to significantly improve plant tolerance. The metabolic mechanism by which AOS induces salt tolerance in rice walls remains unclear. Here, we report impact foliar application on wall seedlings using salt-tolerant variety FL478 salt-sensitive IR29. Data revealed decreased biomass, stem basal width, breaking strength, lodging resistance; however, it increased thickness. In leaves, exogenous up-regulated expression level OSCESA8, abscisic acid (ABA) brassinosteroids (BR) content, β-galacturonic activity, polygalacturonase xylanase laccase cellulose content. Moreover, down-regulated levels OSMYB46 OSIRX10 hemicellulose, pectin, lignin content maintain stability under stress. stems, phenylalamine ammonia-lyase tyrosine activities, while decreasing cellulase, laccase, β-glucanase activities. Furthermore, improved biomass width also enhanced cellulose, stem, As a result, resistance breakage strength alleviated stress-induced damage, thus enhancing resistance. Under stress, regulates phytohormones modifies lignin, pectin metabolism lodging. This study aims alleviate walls, enhance lodging, AOS.

Language: Английский

Citations

4
Spent mushroom substrate: A review on present and future of green applications DOI
Xiaoyu Ma,

Siyu Yan,

Menglu Wang

et al.

Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 373, P. 123970 - 123970

Published: Jan. 1, 2025

Language: Английский

Citations

0
Analysis of fungal microbiota diversity and potential pathogenic fungi in oral secretions and gut feces of captive giant pandas DOI Creative Commons
Xiaoping Ma,

Xiangwen Zeng,

Zhi Huang

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 13, 2025

Maintaining good oral and gut health is essential for the wellbeing of animals, fungi are key components microbiota. This study aims to explore diversity seasonal dynamics fungal communities in captive giant pandas, with a focus on their potential functional roles digestion. In study, we collected saliva fecal samples from 60 pandas were different seasons, analyzed using internal transcribed spacer (ITS) amplicon sequencing. We used α β analyses examine differences species composition among seasons. Furthermore, validated ITS sequencing results through isolation identification. Analyses revealed both similarities between microbiomes pandas. Ascomycota Basidiomycota predominant groups, while dominant genera four seasons Cutaneotrichosporon, unidentified_Chaetothyriales_sp. Additionally, Cladosporium Candida fungus, respectively, across all Notably, abundance microbiome significantly higher than microbiome, pattern observed throughout most Several potentially pathogenic fungi, such as Fusarium, Aspergillus, detected healthy showing increased during winter. It worth mentioning that found distinct bias fungi. The saprophytic relatively high, which may be related role cellulose cavity exhibit significant variations. While hosts community similar intestines. majority likely derived or diet, variation structure further suggests long-term resident not present gut.

Language: Английский

Citations

0
CRISPR/Cas9 mediated targeted knock-in of eglA gene to improve endoglucanase activity of Aspergillus fumigatus LMB-35Aa DOI Creative Commons

J. S. Benites-Pariente,

Ilanit Samolski, Yvette Ludeña

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Aug. 23, 2024

Bioeconomy goals for using biomass feedstock biofuels and bio-based production has arisen the demand fungal strains enzymes processing. Despite well-known Trichoderma Aspergillus commercial strains, continuous bioprospecting revealed biodiversity potential of degrading enzymes. The strain fumigatus LMB-35Aa a great as source lignocellulose-degrading Nevertheless, genetic improvement should be considered to increase its biotechnological potential. Molecular manipulation based on homologous direct recombination (HDR) in filamentous fungi poses challenge since low rate. Currently, CRISPR/Cas9-mediated mutagenesis can enable precise efficient editing genomes. In this study, gene strategy improving endoglucanase activity A. was successfully used, which constitutes first report heterologous cellulase technology. For this, eglA from niger ATCC 10,864 integrated into conidial melanin pksP locus, facilitated selection edited events discerned by emergence albino colonies. Heterologous EglA enzyme biofilm fermentation system resulted 40% mutant compared wild type.

Language: Английский

Citations

3