Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 483, P. 136659 - 136659
Published: Nov. 26, 2024
Language: Английский
Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 483, P. 136659 - 136659
Published: Nov. 26, 2024
Language: Английский
Journal of Environmental Management, Journal Year: 2025, Volume and Issue: 374, P. 124192 - 124192
Published: Jan. 21, 2025
Language: Английский
Citations
1Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 488, P. 137330 - 137330
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Language: Английский
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Language: Английский
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6The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 947, P. 174426 - 174426
Published: July 3, 2024
Language: Английский
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4Environmental Geochemistry and Health, Journal Year: 2024, Volume and Issue: 46(7)
Published: June 7, 2024
Language: Английский
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3Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(11)
Published: Oct. 21, 2024
Language: Английский
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2INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY, Journal Year: 2024, Volume and Issue: 6(2), P. 121 - 130
Published: Oct. 28, 2024
Lipase from Moraxella sp. SBE01 is an expression of the gene encoding lipase. Detection and characterization lipase coding necessary for large-scale production through genetic engineering. This study aimed to observe molecular weight, amino acid sequence, length, conserved acids in DNA gene, with goal identifying characterizing lipase-coding SBE01. The primer design process was conducted amplify using specialized software sequence alignment phylogenetic analysis. Amplification carried out PCR designed primer, forward (GTC ATG TAC TTC CAY GGN GG), reverse (GGT TGC CGC CGG CDS WRT CNC C). under pre-denatured conditions at 95°C (3 minutes), followed by 30 cycles denaturation 95°C, annealing 66°C (30 seconds), 70°C elongations (1 minute) final elongation (10 minutes). results were electrophoresed 1% agarose gel a 1 kb marker. sequenced analyzed sequences type expressed. Sequencing resulted 387 bp nucleotide sequence. had high homology strain TA144. encodes protein consisting 129 contains HGG (His-Gly-Gly) motif, which characteristic lipases family IV, also known as hormone-sensitive (HSL) family. suggests that shares structural functional similarities other enzymes HSL family, playing key role lipid metabolism.
Language: Английский
Citations
0Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 483, P. 136659 - 136659
Published: Nov. 26, 2024
Language: Английский
Citations
0