Microchimica Acta, Journal Year: 2024, Volume and Issue: 192(1)
Published: Dec. 21, 2024
Language: Английский
The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 970, P. 178939 - 178939
Published: March 1, 2025
Multiplex digital PCR (dPCR) approaches are commonly employed in wastewater-based epidemiology (WBE) studies. However, optimizing the dPCR workflow is a critical step to ensure its reliability and accuracy before application. In this study, 6-plex Crystal Digital PCR® (cdPCR) was optimized for simultaneous detection of six epidemiologically important pathogens, including three enteric viruses, noroviruses genogroups I II (NoV-GI GII) enteroviruses (EnV), respiratory severe acute syndrome coronavirus 2 (SARS-CoV-2), which causative agent disease 2019 (COVID-19), influenza A virus (InfA), syncytial B (RSVB), wastewater. Four cDNA input ratios (20 %-70 %) two extraction kits were evaluated optimization, with 30 % AllPrep PowerViral DNA/RNA Kit (Qiagen) exhibiting optimal performance. The cdPCR assay applied year-long wastewater surveillance study Japan (n = 52), revealing distinct trends prevalence viruses. NoV-GII detected 96 samples highest mean concentration (6.1 ± 0.6 log10 copies/L), while SARS-CoV-2 InfA 60 50 samples, respectively, reflected circulation these pathogens within community. Notably, RSVB less frequently (25 %), line fewer cases reported during period. concentrations EnV showed significant positive correlations hand foot mouth herpangina cases, respectively. no observed RSV COVID-19, possibly due testing RSVA more prevalent also cluster outbreaks. These findings demonstrated utility detecting provided insights into community trends, representing an advancement WBE.
Language: Английский
Citations
1
Environmental Pollution, Journal Year: 2025, Volume and Issue: unknown, P. 126298 - 126298
Published: April 1, 2025
Language: Английский
Citations
0
Talanta, Journal Year: 2024, Volume and Issue: 282, P. 127013 - 127013
Published: Oct. 10, 2024
Language: Английский
Citations
3
The Science of The Total Environment, Journal Year: 2025, Volume and Issue: 978, P. 179362 - 179362
Published: April 19, 2025
In recent years, the analysis of viruses in wastewater has become a recognized method to monitor circulation pathogenic within populations. This non-invasive and integrated approach is all more strategic as many viral pathogens can be released sewage. There currently no standard for virological wastewater, various protocols have been developed concentrate detect this matrix. study aims develop high-throughput qPCR detection system describe occurrence 30 human enteric respiratory influent effluent samples collected Paris area during two periods 2023. The influence concentration (ultracentrifugation or aluminium hydroxide adsorption-precipitation) recovery from was also investigated. ultracentrifugation-based allowed higher (4.95-100 % versus 0.77-33 precipitation-based method), especially waters. PCR array revealed presence viruses, with marked seasonal variations mainly viruses. particularly suitable rapid, sensitive, specific multiple wastewater.
Language: Английский
Citations
0
International Journal of Environmental Research and Public Health, Journal Year: 2024, Volume and Issue: 21(11), P. 1432 - 1432
Published: Oct. 29, 2024
During the recent COVID-19 pandemic, wastewater-based epidemiological (WBE) surveillance played a crucial role in evaluating infection rates, analyzing variants, and identifying hot spots community. This expanded possibilities for using wastewater to monitor prevalence of infectious diseases. The full potential WBE remains hindered by several factors, such as lack information on survival pathogens sewage, heterogenicity matrices, inconsistent sampling practices, standard test methods, variable sensitivity analytical techniques. In this study, we review aforementioned challenges, cost implications, process automation, prospects full-fledged community health screening. A comprehensive literature survey was conducted relevant keywords, peer reviewed articles pertinent our research focus were selected with aim serving reference related monitoring early epidemic detection.
Language: Английский
Citations
1
Biosensors, Journal Year: 2024, Volume and Issue: 14(12), P. 633 - 633
Published: Dec. 20, 2024
Antibiotics, celebrated as some of the most significant pharmaceutical breakthroughs in medical history, are capable eliminating or inhibiting bacterial growth, offering a primary defense against wide array infections. However, rise antimicrobial resistance (AMR), driven by widespread use antibiotics, has evolved into and ominous threat to global public health. Thus, creation efficient methods for detecting genes antibiotics is imperative ensuring food safety safeguarding human The clustered regularly interspaced short palindromic repeats (CRISPR) CRISPR-associated proteins (Cas) systems, initially recognized an adaptive immune mechanism bacteria archaea, have unveiled their profound potential sensor detection, transcending notable gene-editing applications. CRISPR/Cas technology employs Cas enzymes guides RNA selectively target cleave specific DNA sequences. This review offers extensive examination highlighting unique attributes applications antibiotic detection. It outlines current utilization progress toolkit identifying both nucleic acid (resistance genes) non-nucleic (antibiotic micromolecules) targets within field In addition, it examines challenges, such sensitivity specificity, future opportunities, including development point-of-care diagnostics, providing strategic insights facilitate curbing oversight antibiotic-resistance proliferation.
Language: Английский
Citations
0
Microchimica Acta, Journal Year: 2024, Volume and Issue: 192(1)
Published: Dec. 21, 2024
Language: Английский
Citations
0