Impact of Sample Storage Time and Temperature on the Stability of Respiratory Viruses and Enteric Viruses in Wastewater DOI Creative Commons
Yuanyuan Qiu,

Richardson Mah,

Logan A. Brand

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2459 - 2459

Published: Nov. 29, 2024

Wastewater-based surveillance (WBS) has been widely used to track SARS-CoV-2 as well many other viruses in communities during the COVID pandemic and post-pandemic. However, it is still not clear how temperature storage time would influence stability of wastewater. In this study, we assessed SARS-CoV-2, pepper mild mottle virus (PMMoV), influenza A (IAV) B (IBV), respiratory syncytial (RSV), enteric raw wastewater stored at room temperature, 4 °C, −20 °C for 3 6 days. PMMoV, IAV, were found be stable up days after storing or °C. RSV more susceptible freeze–thaw cycles compared PMMoV viruses, which relatively Low detection IBV made difficult evaluate impact. Based on our findings, conclude that short-term transportation samples within ambient acceptable majority these viruses. Freezing even short periods recommended WBS The data obtained from study can provide guidance quality assurance purposes operational aspects surveillance.

Language: Английский

COVID-19 Transmission During the Winter 2023-24 Surge: A Comparative Analysis of Surveillance Estimates in the U.S., Canada, and the U.K. DOI Creative Commons
Michael Hoerger, James Gerhart,

Tristen Peyser

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 9, 2025

Abstract Background: Better estimates of COVID-19 transmission are needed since testing has declined. The present investigation examined the correspondence among during winter 2023-24 surge using wastewater-derived for U.S. and Canada testing-derived estimate in U.K. to evaluate validity provide vital public health data on levels. Methods: study used from (Pandemic Mitigation Collaborative dashboard) (COVID-19 Resources testing-based surveillance (Health Security Agency). Data sets were linked by date relative peak within each set. Analyses focused UKHSA period November 2023 March 2024. 1) described day, 2) agreement patterns via correlations, 3) absolute proportion population actively infectious across two months transmission, 4) populations infected months. Results: On day infections, an estimated 1.95 million people U.S., 148 thousand Canada, 431 U.K., meaning 2.5%-4.5% these infectious. Estimates showed high throughout wave, especially between (r=.974, p<.001). During 93.5% 68.8% had excellent or better with data. An >100 months, 20.9%-26.0% population. Discussion: Findings support ongoing significance documenting levels surge. Transmission methodologies nations. More resources prevent diagnose treat long-term sequelae.

Language: Английский

Citations

1
Understanding Nash epidemics DOI Creative Commons
Simon K. Schnyder, John J. Molina, Ryōichi Yamamoto

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2025, Volume and Issue: 122(9)

Published: Feb. 27, 2025

Faced with a dangerous epidemic humans will spontaneously social distance to reduce their risk of infection at socioeconomic cost. Compartmentalized models have been extended include this endogenous decision making: Individuals choose behavior optimize utility function, self-consistently giving rise population behavior. Here, we study the properties resulting Nash equilibria, in which no member can gain an advantage by unilaterally adopting different We leverage analytic solution that yields fully time-dependent rational obtain, 1) simple relationship between distancing and current number infections; 2) scaling results for how peak total cases depend on cost contracting disease; 3) characteristic costs divide regimes strong weak behavioral response; 4) closed form expression value utility. discuss these provide deep intuitive understanding disease dynamics, useful both individuals policymakers. In particular, infections represents heuristic could be communicated encourage, or “bootstrap,”

Language: Английский

Citations

0
Monitoring the Emergence of SARS-CoV-2 VOCs in Wastewater and Clinical Samples—A One-Year Study in Santiago de Compostela (Spain) DOI Creative Commons
Marta Lois, David Polo, María de Toro

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 489 - 489

Published: March 28, 2025

Wastewater surveillance has become a valuable tool to monitor the emergence of SARS-CoV-2 variants concern (VOCs) at community level. In this study, we aimed evaluate presence Alpha (B.1.1.7), Beta (B.1.351), Delta (B.1617.2), and Omicron (B.1.1.529) VOCs in samples from inlet wastewater treatment plant (WWTP) as well two different sewer interceptors (SI-1 SI-2) urban sewage system Santiago de Compostela (Galicia, NW Spain) throughout 2021 January 2022. For purpose, detection quantification four was performed using duplex allelic discrimination RT-qPCR assays, targeting S-gene. An N1 gene assay used reference for RNA samples. All were detected Alpha, Beta, Delta, 45.7%, 7.5%, 66.7%, 72.7% all samples, respectively. VOC dominant during first part whereas peaks observed May–June December 2021, Some differences among results obtained city sectors studied, which could be explained by characteristics population between them. Wastewater-based epidemiology allowed us track early circulation local level, our are temporally concordant with clinical data epidemiological findings reported health authorities.

Language: Английский

Citations

0
Adaptive human behaviour modulates the impact of immune life history and vaccination on long-term epidemic dynamics DOI
Baltazar Espinoza, Chadi M. Saad-Roy, Bryan T. Grenfell

et al.

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 291(2033)

Published: Oct. 1, 2024

The multiple immunity responses exhibited in the population and co-circulating variants documented during pandemics show a high potential to generate diverse long-term epidemiological scenarios. Transmission variability, immune uncertainties human behaviour are crucial features for predictability implementation of effective mitigation strategies. Nonetheless, effects individual health incentives on disease dynamics not well understood. We use behavioural-immuno-epidemiological model study joint evolution epidemic different Our results reveal trade-off between individuals’ levels behavioural produced. find that adaptive can avoid dynamical resonance by avoiding large outbreaks, producing subsequent uniform outbreaks. forward-looking shows an optimal planning horizon minimizes burden balancing risk–benefit trade-off. compensate differential levels, equalizing scenarios with underlying landscapes. adequately capture complex empirical observed pandemics. tested our US states COVID-19 pandemic. Finally, we explored extensions modelling framework incorporate lockdowns, emergence novel variant, prosocial attitudes pandemic fatigue.

Language: Английский

Citations

3
Impact of Sample Storage Time and Temperature on the Stability of Respiratory Viruses and Enteric Viruses in Wastewater DOI Creative Commons
Yuanyuan Qiu,

Richardson Mah,

Logan A. Brand

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2459 - 2459

Published: Nov. 29, 2024

Wastewater-based surveillance (WBS) has been widely used to track SARS-CoV-2 as well many other viruses in communities during the COVID pandemic and post-pandemic. However, it is still not clear how temperature storage time would influence stability of wastewater. In this study, we assessed SARS-CoV-2, pepper mild mottle virus (PMMoV), influenza A (IAV) B (IBV), respiratory syncytial (RSV), enteric raw wastewater stored at room temperature, 4 °C, −20 °C for 3 6 days. PMMoV, IAV, were found be stable up days after storing or °C. RSV more susceptible freeze–thaw cycles compared PMMoV viruses, which relatively Low detection IBV made difficult evaluate impact. Based on our findings, conclude that short-term transportation samples within ambient acceptable majority these viruses. Freezing even short periods recommended WBS The data obtained from study can provide guidance quality assurance purposes operational aspects surveillance.

Language: Английский

Citations

0