Maternal body condition affects the response of larval spined toads’ faecal microbiome to a widespread contaminant DOI Open Access
Sabrina Tartu, Nicolas Pollet,

Isabelle Clavereau

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 18, 2023

Abstract Glyphosate’s primary metabolite, aminomethylphosphonic acid (AMPA), is the most detected pollutant in surface waters. Recent studies have raised concerns about its toxicity, yet underlying mechanisms remain poorly understood. A disruption of gut microbiome, which plays a crucial role host health, could mediate adverse effects. We investigated impact AMPA exposure on microbiome spined toad tadpoles ( Bufo spinosus ). hypothesized that alter microbiota composition and these effects depend source. exposed to minute concentrations analyzed their faecal using 16S rRNA gene sequencing as proxy microbiota. decreased bacterial biomass affected community tadpole’s faeces. Furthermore, we observed interactions between maternal body condition Bacteroidota Actinobacteriota phyla abundances. This suggests effect early-life microbial colonizers influence response AMPA. These findings highlight importance considering when studying environmental contaminants. Further research needed elucidate long-term implications this alteration for amphibian health.

Language: Английский

Hawthorn-leaf flavonoid alleviate intestinal health and microbial dysbiosis problems induced by glyphosate DOI Creative Commons
Hongjian Dai, Jiao Wang, Yujie Li

et al.

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 284, P. 116901 - 116901

Published: Aug. 22, 2024

Glyphosate is the active ingredient in herbicide (i.e., Roundup, Touchdown and Erasure), safety of which has become a social concern. Hawthorn-leaf flavonoid (HF) possesses various biological functions, including antioxidant, regulating lipid metabolism intestinal microbiota. Whether HF could reduce health risk pure glyphosate to birds remain unknown. The experiment aimed evaluate effects (25 mg/kg added water) on microbiota chicks protective roles (60 diet). Exposure decreased growth performance, ileal morphology structure, antioxidant capacity, increased serum level pro-inflammatory factors. 16S rRNA sequencing indicated that bacterial richness abundance Lactobacillus, proportions pathogens ileum. Metabolomic results revealed cholic acid fatty acids ileac digesta. Meanwhile, down-regulated protein expression associated with transport, tight junction mucosal tissue, up-regulated pro-inflammatory, oxidative stress proteins. However, dietary supplementation effectively mitigated adverse improved chicks. Therefore, can ameliorate harmful birds, highlights potential application reducing risks.

Language: Английский

Citations

1
Roundup-Induced Gut Dysbiosis, Irrelevant to Aromatic Amino Acid Deficiency, Impairs the Gut Function in Rats DOI

Tianchi Duan,

Aamina Alim,

Honglei Tian

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 20, 2024

Glyphosate, the most popular herbicide globally, has long been considered safe for mammals. However, whether glyphosate can disturb gut microbiota via inhibiting aromatic amino acid (AAA) synthesis under debate recently. Here, we evaluated impacts of chronic exposure to Roundup on health with addition AAA and explored mechanism behind Roundup-induced dysfunction by performing fecal transplantation. 500 mg/kg·bw Roundup, independent deficiency, caused severe damage function, as characterized microbial dysbiosis, oxidative stress damage, intestinal inflammation, histopathological injury, particularly in female rats. Notably, similar Roundup-shaped microbiome evidently damaged serum, cecum, colon profiling biomarkers (malonaldehyde (MDA), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), (GSH), H

Language: Английский

Citations

1
Urinary biomonitoring of exposure to glyphosate and its metabolite amino-methyl phosphonic acid among farmers and non-farmers in Morocco DOI
Zineb Ben Khadda,

Simona Bungău,

Souleiman El Balkhi

et al.

Environmental Toxicology and Pharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 104620 - 104620

Published: Dec. 1, 2024

Language: Английский

Citations

1
Association of urine glyphosate levels with renal injury biomarkers in children living close to major vegetable-producing regions in China DOI
Jingbo Liu, Lei Wang, Li Song

et al.

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 912, P. 168677 - 168677

Published: Nov. 24, 2023

Language: Английский

Citations

2
Impact de l’exposition au glyphosate sur la neuroplasticité – de la mère à la descendance DOI
Thierry Charlier

Cahiers de Nutrition et de Diététique, Journal Year: 2024, Volume and Issue: 59(2), P. 128 - 139

Published: Jan. 19, 2024

Citations

0
Associations between urine glyphosate levels and metabolic health risks: insights from a large cross-sectional population-based study DOI Creative Commons

Sarah Otaru,

Laura Jones, David O. Carpenter

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 24, 2024

Abstract Background: Prevalence of the Metabolic Syndrome (MetS) in American adults has risen from 37.6% 2011-12 period to 41.8% 2017-2018. Environmental exposure, particularly common compounds such as glyphosate, drawn increasing attention a potential risk factor. Methods: We employ three cycles data (2013-2018) National Health and Nutrition Examination Survey (NHANES) cross-sectional study examine associations between urine glyphosate measurements MetS. We first created MetS score using Exploratory Factor Analysis 6 International Diabetes Federation (IDF) criteria for MetS, with 2013-2018 NHANES cycles, validated this independently on an additional associated metric, Albumin Creatinine Ratio. The was via machine-learning approach predicting ACR binary classification, then used multivariable regression test association quartile-categorized exposure score. Results: In adjusted regressions, quartile regressions show significant inverted U-shaped or saturating dose-response profile, often largest effect exposures 3. Exploration modification by sex, race, age category shows differences race age, older people (ages > 65 years) non-Hispanic African participants showing larger sizes all quartiles. Conclusions: find that urinary is significantly statistical designed capture nonlinear, advanced Mexican other Hispanic higher sizes.

Language: Английский

Citations

0
Cambio climático y plaguicidas: el caso del glifosato DOI Creative Commons
Merle M. Borges-Ramírez, Ricardo Dzul-Caamal, Jaime Rendón‐von Osten

et al.

JAINA Costas y Mares ante el Cambio Climático, Journal Year: 2024, Volume and Issue: 6(1), P. 85 - 96

Published: Nov. 1, 2024

esticide pollution, such as that caused by glyphosate, affects marine and terrestrial ecosystems globally. This agrochemical pollution is exacerbated climate change, which leads to fluctuations in temperatures increases greenhouse gases. These factors stress organisms their microbiomes. Additionally, the change forces adapt changes precipitation patterns, resulting droughts floods. Consequently, use of pesticides has also changed, often leading application larger quantities than were previously required, due growing resistance some pests. It been documented driven many migrate geographically. The interaction between pesticide uses temperature promotes plant diseases, reducing availability quality food, while causing damage reproduction certain organisms, insects, amphibians, fish, among others. In case its intensive widespread use—primarily genetically modified seeds—has led contamination virtually all with this compound, including humans. Keywords: Pesticides, Glyphosate, Climate change.

Language: Английский

Citations

0
Revealing novel protein interaction partners of glyphosate in Escherichia coli DOI Creative Commons
Alix Sarah Aldehoff, Dominique Türkowsky,

Patrick Lohmann

et al.

Environment International, Journal Year: 2024, Volume and Issue: 195, P. 109243 - 109243

Published: Dec. 25, 2024

Despite all debates about its safe use, glyphosate remains the most widely applied active ingredient in herbicide products, with renewed approval European Union until 2033. Non-target organisms are commonly exposed to as a matter of mode application, broader environmental and biological impacts remaining under investigation. Glyphosate displays structural similarity phosphoenolpyruvate (PEP), thereby competitively inhibiting 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS), crucial for synthesis aromatic amino acids plants, fungi, bacteria, archaea. Most microbes, including gut bacterium Escherichia coli (E. coli), possess glyphosate-sensitive class I EPSPS, making them vulnerable glyphosate's effects. Yet, little is known interactions other bacterial proteins or modes action at proteome level. Here, we employed quantitative proteomics thermal profiling (TPP) approach identify novel protein binding partners E. proteome. exposure significantly altered acid synthesizing pathways. The abundance shikimate pathway was increased, suggesting compensatory mechanism. Extracellular riboflavin concentrations were elevated upon exposure, while intracellular levels remained stable. Beyond target enzyme indicated an effect on stability certain proteins, AroH ProA, indicating interactions. Similar competitive between PEP one reason interaction ProA could be high their substrates glyphosate. Overall, induced metabolic disturbances coli, extending beyond primary target, providing new insights into impact microbial systems.

Language: Английский

Citations

0
Maternal body condition affects the response of larval spined toads’ faecal microbiome to a widespread contaminant DOI Open Access
Sabrina Tartu, Nicolas Pollet,

Isabelle Clavereau

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 18, 2023

Abstract Glyphosate’s primary metabolite, aminomethylphosphonic acid (AMPA), is the most detected pollutant in surface waters. Recent studies have raised concerns about its toxicity, yet underlying mechanisms remain poorly understood. A disruption of gut microbiome, which plays a crucial role host health, could mediate adverse effects. We investigated impact AMPA exposure on microbiome spined toad tadpoles ( Bufo spinosus ). hypothesized that alter microbiota composition and these effects depend source. exposed to minute concentrations analyzed their faecal using 16S rRNA gene sequencing as proxy microbiota. decreased bacterial biomass affected community tadpole’s faeces. Furthermore, we observed interactions between maternal body condition Bacteroidota Actinobacteriota phyla abundances. This suggests effect early-life microbial colonizers influence response AMPA. These findings highlight importance considering when studying environmental contaminants. Further research needed elucidate long-term implications this alteration for amphibian health.

Language: Английский

Citations

0