Both acute glyphosate and the aminomethylphosphonic acid intoxication decreased the acetylcholinesterase activity in rat hippocampus, prefrontal cortex and gastrocnemius muscle

Drug and Chemical Toxicology, Journal Year: 2024, Volume and Issue: 47(6), P. 1033 - 1037

Published: March 11, 2024

It has been reported that glyphosate, one of the most common herbicides used in agriculture, impairs locomotion and cognition. Glyphosate a variable half-life soil up to biotic and/or abiotic factors transform molecule metabolites such as aminomethylphosphonic acid (AMPA) longer half-life. In this study, female Sprague Dawley rats were acutely exposed different doses glyphosate or AMPA (i.e. 10, 56 100 mg/kg) and, subsequently, acetylcholinesterase (AChE) activity was measured hippocampus, prefrontal cortex (PFC) gastrocnemius muscle. Both produced similar decrease AChE all tissues tested. These results suggest interference with normal cholinergic neurotransmission may be mechanisms involved glyphosate-induced motor alterations rats. Moreover, our highlight biological importance anticholinesterase action brain skeletal To knowledge, is first report showing

Language: Английский

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The relationship between urinary glyphosate and all-cause and specific-cause mortality: a prospective study

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 28, 2025

Glyphosate (GLY) is a well-known herbicide with significant applications in both agriculture and non-agriculture. However, GLY overuse recent years has resulted detection of residues many crops, endangering human health food safety. Our aim to investigate the relationship between urinary mortality, as well its influencing factors. The National Health Nutrition Examination Survey (NHANES) data from 4740 American adults were examined. Fitted smooth curves, generalized summation models, multiple logistic regression models used mortality. To potential regulatory elements two effects, perform subgroup analysis. During median follow-up 4.03 years, there total 238 all-cause deaths, 75 cardiovascular disease (CVD) deaths 52 cancer deaths. positively correlated Each 1 ng/ml increase was associated 40% increased risk mortality (Hazard ratio (HR) 1.40, 95% confidence interval (CI) 1.09-1.80), an 50% High group compared Low (HR 1.50, CI 1.05-2.14). In analysis, association significantly modified by gender (P for interaction = 0.03), hypertension 0.022). Higher seems be more death, may affect this association. Furthermore, urine have higher effect on people without hypertension.

Language: Английский

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Glyphosate Induces Anxiety‐Like Behaviors in Mice via Activating NLRP3‐Mediated Hippocampal Microglia Pyroptosis

Journal of Applied Toxicology, Journal Year: 2025, Volume and Issue: unknown

Published: April 20, 2025

ABSTRACT Glyphosate is reported to compromise the central nervous system and induce behavioral disorders in mammals. However, evidence deficient with respect potential mechanisms involved. In this study, C57BL/6 mice were orally exposed glyphosate at doses ranging from 0 200 mg/kg/day for 16 weeks. The results of tests showed that decreased time spent distance traveled within area open field test, retention number entries into arms, increased close arms elevated plus maze test. expressions anxiety‐related genes htr5 , htr6 tmem132d also significantly upregulated by glyphosate. This phenotype was linked permeation brain via disrupting functional tight junctions blood–brain barrier (BBB). As a result, directly compromised morphological structure neurons, IBA‐1 microglia, activated expression NLRP3‐mediated pyroptosis pathway (NLRP3, Caspase‐1, GSDMD, IL‐18) hippocampus mice. Moreover, glyphosate‐induced activation NLRP3 microglia markedly reversed inhibitor MCC950. conditional mediums glyphosate‐treated BV2 cells aggravated cytotoxicity HT‐22 which rescued conclusion, work demonstrated microglia‐mediated plays detrimental role glyphosate‐behavioral neuron damage. These findings provide novel neurotoxicity support growing association between exposure neurobehavioral humans.

Language: Английский

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Aminomethylphosphonic Acid (AMPA), a Glyphosate Metabolite, Decreases Plasma Cholinesterase Activity in Rats

Journal of Xenobiotics, Journal Year: 2024, Volume and Issue: 14(2), P. 604 - 612

Published: May 7, 2024

Glyphosate, a widely used herbicide, is linked to plethora of deleterious effects in both clinical and preclinical studies. Nevertheless, the its main metabolite, aminomethylphosphonic acid (AMPA), whose half-life soil even longer than that glyphosate, have been little explored. On this basis, as first approach, work, we report intraperitoneal (i.p.) administration AMPA or glyphosate (at 10, 56, 100 mg/kg) decreased, similar extent, plasma cholinesterase (ChE) activity acutely exposed rats. Moreover, designed an experimental protocol analyze compare on human ChE activity; consisted adding these compounds subsequently test contraction acetylcholine (ACh) frog rectus abdominis muscle (an indirect estimate activity). Accordingly, muscular ACh was evaluated before after pre-incubation with (i) alone, (ii) AMPA, (iii) glyphosate. Our results indicate like decreased rats (when given i.p.) humans added vitro), suggesting xenobiotics may exert toxicological effects.

Language: Английский

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Tannic acid inhibits pain mediators, inflammation and oxidative stress in mice exposed to glyphosate-based herbicide

Environmental Analysis Health and Toxicology, Journal Year: 2024, Volume and Issue: 39(2), P. e2024019 - e2024019

Published: June 21, 2024

Chronic exposure to glyphosate-based herbicide (Gly) has been associated with neurological disorders. Tannic acid (TA) is an antioxidant attenuating action against neuroinflammation-associated conditions. This study evaluated the effect of Gly on pain perception alongside antinociceptive and anti-inflammatory actions TA in Gly-exposed mice. Male Swiss mice were randomly divided into six groups (n=8): control (distilled water 0.2 ml/kg), (Gly 500 mg/kg), Pre-TA + (TA 50 mg/kg pre-treatment, afterwards Gly-administered), co-administered), Pre-AA (ascorbic (AA) 10 AA (AA co-administered). Mechanical, thermal, chemical weeks post vehicle/drugs administrations orally, followed by brain biochemical measurements. treatment alleviated Gly-induced hyperalgesia similar version values increasing significantly (p < 0.05) nociceptive thresholds. Moreover, TA-treatment decreased malondialdehyde (MDA) pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) levels, increased (IL-10, IL-4, TGF-1β) enzymes, catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) activities compared Gly-treated 0.05). Conclusively, exerted actions, possibly through its Notably, pre-treatment showed a better response than co-administration. We propose potential neuroprotective ameliorative functions hyperalgesia. merits further clinical research protective roles pesticide-related

Language: Английский

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Serum N-Glycan Changes in Rats Chronically Exposed to Glyphosate-Based Herbicides

Biomolecules, Journal Year: 2024, Volume and Issue: 14(9), P. 1077 - 1077

Published: Aug. 28, 2024

Glyphosate, the active ingredient in many herbicides, has been widely used agriculture since 1970s. Despite initial beliefs its safety for humans and animals due to absence of shikimate pathway, recent studies have raised concerns about potential health effects. This study aimed identify glycomic changes indicative glyphosate-induced toxicity. Specifically, focused on profiling

Language: Английский

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Urinary biomonitoring of exposure to glyphosate and its metabolite amino-methyl phosphonic acid among farmers and non-farmers in Morocco

Environmental Toxicology and Pharmacology, Journal Year: 2024, Volume and Issue: unknown, P. 104620 - 104620

Published: Dec. 1, 2024

Language: Английский

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Neuroglycome alterations of hippocampus and prefrontal cortex of juvenile rats chronically exposed to glyphosate-based herbicide

Frontiers in Neuroscience, Journal Year: 2024, Volume and Issue: 18

Published: Aug. 21, 2024

Introduction Glyphosate-based herbicides (GBHs) have been shown to significant neurotoxic effects, affecting both the structure and function of brain, potentially contributing development neurodegenerative disorders. Despite known importance glycosylation in disease progression, glycome profile systems exposed GBH has not thoroughly investigated. Methods In this study, we conducted a comprehensive glycomic profiling using LC-MS/MS, on hippocampus prefrontal cortex (PFC) juvenile rats orally, aiming identify glyco-signature aberrations after herbicide exposure. Results We observed changes profile, particularly fucosylated, high mannose, sialofucosylated N-glycans, which may be triggered by Moreover, found major differences N-glycan profiles between GBH-exposed group control when analyzing each gender independently, contrast analysis that included genders. Notably, behavioral test object recognition showed decreased performance female animals compared controls ( p &lt; 0.05), while normal behavior was recorded male &gt; 0.05). Conclusion These findings suggest glycans play role effect caused GBH. The result suggests variation influence response exposure, with potential implications for progression specifically effects GBHs. Understanding these gender-specific responses could enhance knowledge mechanisms underlying GBH-induced toxicity its impact brain health. Overall, our study represents first detailed N-glycome PFC chronically alterations expression structures associated chronic highlighting further research area.

Language: Английский

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Acute glyphosate and aminomethylphosphonic acid (AMPA), its major metabolite, impaired spatial orientation, navigation, learning and/or memory in female rats

Behavioural Brain Research, Journal Year: 2024, Volume and Issue: 478, P. 115329 - 115329

Published: Nov. 8, 2024

Language: Английский

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