Molecular Investigation of gyrA Mutations in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus Derived from Diverse Sources DOI Creative Commons

Safaa Ehssan Atta,

Lujain Ghannawi,

Omar Yasir Shakir

et al.

Al-Rafidain Journal of Medical Sciences ( ISSN 2789-3219 ), Journal Year: 2023, Volume and Issue: 5(1S), P. S51 - 57

Published: Nov. 3, 2023

Background: Fluoroquinolones are the most effective antibiotics against Staphylococcus aureus isolates. In hospitals, excessive use of has led to emergence highly resistant strains S. Objective: The aim this study was detect mutations that occur in gyrA gene encoding for DNA gyrase, which is one targets fluoroquinolone resistance. Methods: Fifty clinical isolates were diagnosed as according molecular and bacteriological methods. susceptibility tests performed on all bacterial by disc diffusion method using methicillin six antibiotics. Results: Out fifty isolates, twelve (nalidixic acid, lomefloxacin, ciprofloxacin, norfloxacin, ofloxacin, levofloxacin). From 12 resistant, 3 intermediate, 38 sensitive three or more tested resistance also confirmed minimum inhibitory concentration test. main sources burns (10%), nose (16) wounds (8%), operation room ear (20%), urine skin (6%), throat (22%). Twelve used examine gene. A direct sequence analysis found eight gene; these included 2 (25% missense mutations), 1 (12.5%) deletion mutation, 5 (62.5%) silent at various sites. Conclusion: resulting from may be mechanisms leading

Language: Английский

Emerging Bi-based perovskites photocatalysts for degradation of pharmaceutical compounds: Insights into active sites, potential industrial application, and new approaches DOI
Artem S. Belousov,

Е. В. Сулейманов,

Iqrash Shafiq

et al.

Journal of environmental chemical engineering, Journal Year: 2025, Volume and Issue: unknown, P. 116750 - 116750

Published: April 1, 2025

Language: Английский

Citations

0
Excellent phenol removal ability of Acinetobacter guillouiae BY18 under different inorganic nitrogen conditions DOI Creative Commons
Yang Li, Tengxia He, Mengping Chen

et al.

Environmental Technology & Innovation, Journal Year: 2024, Volume and Issue: 35, P. 103694 - 103694

Published: May 31, 2024

Phenol and inorganic nitrogen generally exist in industrial wastewater contaminated soil. In this study, Acinetobacter guillouiae BY18, which can simultaneously remove high concentrations of phenol nitrogen, was isolated from a wet meadow Weining County, Guizhou Province, China. The optimal remediation conditions for strain BY18 were 25 °C pH 7.20–8.20, with C/N ratio 10, rotation speed 150 r/min, inoculation amount 0.2×108 CFU. When ammonium both present, could 982.86 mg/L 64.76 within 18 h. Strain able to eliminate 979.71 mg/L, 55.84 49.20 phenol, nitrite, nitrate, respectively, 36 h when substituted by nitrate or nitrite. the simultaneous nitrification denitrification reaction system, maximum removal rates 92.53 mg/(L·h) 5.7 mg/(L·h), respectively. Overall, had remarkable ability under different conditions.

Language: Английский

Citations

1
Impact of Novel Remediation Technology: Significant Role in the Removal of Toxic Pollutants via Sustainable Approaches DOI

A. Sankaranarayanan,

K. R. Jeya,

M. Veerapagu

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Molecular Investigation of gyrA Mutations in Clinical Isolates of Methicillin-Resistant Staphylococcus aureus Derived from Diverse Sources DOI Creative Commons

Safaa Ehssan Atta,

Lujain Ghannawi,

Omar Yasir Shakir

et al.

Al-Rafidain Journal of Medical Sciences ( ISSN 2789-3219 ), Journal Year: 2023, Volume and Issue: 5(1S), P. S51 - 57

Published: Nov. 3, 2023

Background: Fluoroquinolones are the most effective antibiotics against Staphylococcus aureus isolates. In hospitals, excessive use of has led to emergence highly resistant strains S. Objective: The aim this study was detect mutations that occur in gyrA gene encoding for DNA gyrase, which is one targets fluoroquinolone resistance. Methods: Fifty clinical isolates were diagnosed as according molecular and bacteriological methods. susceptibility tests performed on all bacterial by disc diffusion method using methicillin six antibiotics. Results: Out fifty isolates, twelve (nalidixic acid, lomefloxacin, ciprofloxacin, norfloxacin, ofloxacin, levofloxacin). From 12 resistant, 3 intermediate, 38 sensitive three or more tested resistance also confirmed minimum inhibitory concentration test. main sources burns (10%), nose (16) wounds (8%), operation room ear (20%), urine skin (6%), throat (22%). Twelve used examine gene. A direct sequence analysis found eight gene; these included 2 (25% missense mutations), 1 (12.5%) deletion mutation, 5 (62.5%) silent at various sites. Conclusion: resulting from may be mechanisms leading

Language: Английский

Citations

1