The shared mechanism and potential diagnostic markers for premature ovarian failure and dry eye disease DOI Creative Commons
Xi Long, Zixuan Wu, Pengfei Jiang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 13, 2024

Abstract Premature ovarian failure (POF), which is often comorbid with dry eye disease (DED) a key issue affecting female health. Here, we explored the mechanism underlying POF and DED to further elucidate mechanisms improve treatment. Datasets related (GSE39501) (GSE44101) were identified from Gene Expression Omnibus (GEO) database subjected weighted gene coexpression network (WGCNA) differentially expressed genes (DEGs) analyses, respectively, intersection used obtain 158 in DED. Kyoto Encyclopedia of Genes Genomes (KEGG) Ontology (GO) analyses revealed that primarily DNA replication Cell cycle, respectively. Protein–Protein interaction (PPI) analysis obtained 15 hub genes: CDC20, BIRC5, PLK1, TOP2A, MCM5, MCM6, MCM7, MCM2, CENPA, FOXM1, GINS1, TIPIN, MAD2L1, CDCA3. To validate results, additional POF- DED-related datasets (GSE48873 GSE171043, respectively) selected. miRNAs-lncRNAs-genes machine learning methods genes. The DGIdb valdecoxib, amorfrutin A, kaempferitrin as potential drugs. Herein, bioinformatics perspective, providing new strategy explore comorbidity mechanism, opening up direction for diagnosis treatment

Language: Английский

Eye‐Drop Nano‐Formulation of Catalase Self‐Assembled with Thiolated Chitosan for Effective Treatment of Dry Eye Disease DOI Open Access

Ming Shao,

Yu Chai,

Yutong Jiang

et al.

Advanced Materials, Journal Year: 2025, Volume and Issue: unknown

Published: March 23, 2025

Abstract Dry eye disease (DED), the most prevalent ophthalmological condition worldwide, can cause severe ocular discomfort and even visual impairment. Effective yet safe therapeutics for DED are still highly demanded. Herein, considering important role of excessive reactive oxygen species (ROS) in triggering DED, an eye‐drop nano‐formulation catalase (CAT) self‐assembled with cysteine‐modified chitosan (CS‐Cys) is designed treatment. Upon administration CS‐Cys/CAT nanoparticles, CS‐Cys form disulfide bonds abundant thiols mucin layer tear film, anchoring to corneal surface. Thus excess ROS accumulated on surface be effectively eliminated, resulting a regulated microenvironment. In mouse rabbit models, it verified that drops offer excellent therapeutic effects, especially promoting recovery damaged epithelium increasing secretion. Remarkably, showed notably better performance than clinically used cyclosporin dexamethasone, as well several new drugs clinical trials. Thus, work presents unique nanoparticulate eye‐drop‐based delivery system allow prolonged retention protein therapeutics, such nanoformulation formulated by fully biocompatible/biodegradable components possesses significant translational potential effective

Language: Английский

Citations

0
Single-Nuclei Characterization of Lacrimal Gland in Scopolamine-Induced Dry Eye Disease DOI Creative Commons
Yang Tang, Shengqian Dou, Chao Wei

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(4), P. 46 - 46

Published: April 30, 2024

The lacrimal gland (LG) is the main organ responsible for tear secretion and an important pathogenic site dry eye disease (DED). This study aimed to comprehensively characterize LG cellular heterogeneity under normal DED conditions using single-nucleus RNA sequencing (snRNA-seq).

Language: Английский

Citations

3
Rodent models for dry eye syndrome (DES) DOI
Jingyun Zhu,

Liu Liu,

Jian Wu

et al.

Contact Lens and Anterior Eye, Journal Year: 2025, Volume and Issue: unknown, P. 102383 - 102383

Published: Feb. 1, 2025

Language: Английский

Citations

0
CGRP Released by Corneal Sensory Nerve Maintains Tear Secretion of the Lacrimal Gland DOI Creative Commons

Linyan Ma,

Lingling Yang, Xiaolei Wang

et al.

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(4), P. 30 - 30

Published: April 18, 2024

Purpose: This study aims to elucidate the calcitonin gene-related peptide (CGRP) mediation and primary mechanism of corneal sensory nerves on tear production lacrimal gland. Methods: Mouse denervation models were constructed through surgical axotomy, pharmacologic treatment with capsaicin or resiniferatoxin, Trpv1-Cre/DTR mice diphtheria toxin injection. The capsaicin-treated received subconjunctival injection CGRP substance P, while normal C57BL/6J administered receptor antagonist BIBN-4096. Furthermore, double immunostaining c-FOS+ choline acetyltransferase was used evaluate activation superior salivatory nucleus (SSN). glands collected for transcriptomic sequencing subsequent RNA protein expression analysis. Results: denervated exhibited a significant reduction in sensitivity secretion. In mice, secretion decreased 2.5 ± 0.5 mm compared 6.3 0.9 control (P < 0.0001). However, exogenous administration increased from 2.6 4.5 = 0.0009), BIBN-4096 reduced 3.4 when 7.3 0.7 0.0022). cell number SSN by twofold 0.0168) after mice. addition, expressions CCNA2, Ki67, PCNA, CDK1 acinar cells gland impaired alleviated administration. Conclusions: released mediates gland, providing new strategy improving patients neurotrophic keratitis.

Language: Английский

Citations

2
Restoration of corneal epithelial barrier function: A possible target for corneal neovascularization DOI
Sitong Shen, Yan Zhang

The Ocular Surface, Journal Year: 2024, Volume and Issue: 34, P. 38 - 49

Published: June 18, 2024

Language: Английский

Citations

2
The shared mechanism and potential diagnostic markers for premature ovarian failure and dry eye disease DOI Creative Commons
Xi Long, Zixuan Wu, Pengfei Jiang

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: July 13, 2024

Abstract Premature ovarian failure (POF), which is often comorbid with dry eye disease (DED) a key issue affecting female health. Here, we explored the mechanism underlying POF and DED to further elucidate mechanisms improve treatment. Datasets related (GSE39501) (GSE44101) were identified from Gene Expression Omnibus (GEO) database subjected weighted gene coexpression network (WGCNA) differentially expressed genes (DEGs) analyses, respectively, intersection used obtain 158 in DED. Kyoto Encyclopedia of Genes Genomes (KEGG) Ontology (GO) analyses revealed that primarily DNA replication Cell cycle, respectively. Protein–Protein interaction (PPI) analysis obtained 15 hub genes: CDC20, BIRC5, PLK1, TOP2A, MCM5, MCM6, MCM7, MCM2, CENPA, FOXM1, GINS1, TIPIN, MAD2L1, CDCA3. To validate results, additional POF- DED-related datasets (GSE48873 GSE171043, respectively) selected. miRNAs-lncRNAs-genes machine learning methods genes. The DGIdb valdecoxib, amorfrutin A, kaempferitrin as potential drugs. Herein, bioinformatics perspective, providing new strategy explore comorbidity mechanism, opening up direction for diagnosis treatment

Language: Английский

Citations

1