Organophospate-Induced Pathology: Mechanisms of Development, Principles of Therapy and Features of Experimental Studies DOI
Н. В. Гончаров, Daria A. Belinskaia, П. В. Авдонин

et al.

Российский физиологический журнал им  И  М  Сеченова, Journal Year: 2023, Volume and Issue: 109(10), P. 1349 - 1397

Published: Oct. 1, 2023

Organophosphates (OP) are one of the most common neurotoxic xenobiotics. In acute OP poisoning, as a result suppression synaptic acetylcholinesterase (AChE) activity, cholinergic syndrome develops, which can transform into status epilepticus. Within few days after so-called an intermediate develop, is associated with prolonged inhibition AChE, desensitization nicotinic receptors, and functional degradation synapses muscle fibers. 10–20 single or repeated subacute OP-induced delayed polyneuropathy (OPIDN) develop – neurodegenerative disease, signs ataxia, loss function distal sensory motor axons peripheral nerves. The occurrence neuropsychiatric disorder (NPD) caused by chronic exposure to relatively low-toxicity organophosphorus compounds usually not poisoning; symptoms include cognitive impairment, fatigue, extrapyramidal symptoms. list possible diseases pathological conditions (syndromes) that acute, effects on human body has expanded in recent years due number known (Alzheimer’s, Parkinson’s, multiple sclerosis, etc.). aging general brain particular considered review from point view consequences serve nonspecific trigger related diseases. Gulf consequence intoxication, but also interest context pathology, since its etiology pathogenesis cholinesterase inhibitors. presents data indicating important role vascular endothelium development pathology; first suggestions were made clinicians late 1980s, experimental obtained early 2000s. principles therapy for poisoning outlined, taking account years. Some methods studying experiments vitro, ex vivo laboratory animals, including use carboxylesterase inhibitors, presented. part investigations been remains search new biomarkers assess effectiveness adjuvant regenerative therapies.

Language: Английский

Can Caenorhabditis elegans Serve as a Reliable Model for Drug and Nutraceutical Discovery? DOI Creative Commons
Opeyemi Deji-Oloruntoba, Taiwo O. Elufioye, Stephen A. Adefegha

et al.

Applied Biosciences, Journal Year: 2025, Volume and Issue: 4(2), P. 23 - 23

Published: May 2, 2025

Experimental research demands the selection of appropriate models to align with study objectives and conditions. Traditional experimental models, such as in vivo animal studies vitro systems like organoids, present nutraceutical pharmaceutical limitations high cost, ethical concerns, long lifespan, difficult genetic manipulation. Caenorhabditis elegans has proved be a valuable model result its physiological similarities higher organisms, fully sequenced genome, short life cycle, transparency. These features enable high-throughput screening, molecular pathway analysis, lifespan healthspan assays. C. significantly advanced discovery bioactive molecules therapeutic potential, shedding light on aging, neurodegeneration, metabolic disorders, immune responses. Its utility pharmacokinetics validation nutraceuticals underscores role longevity health research. Additionally, conserved stress response, apoptosis, pathogen recognition pathways facilitate pharmacological interventions for inflammation, oxidative stress, infections. This evaluates applicability analyses drug efficacy testing, discusses relevant advancements, associated difficulties, what expect

Language: Английский

Citations

0
Short lifespan is one’s fate, long lifespan is one’s achievement: lessons from Daphnia DOI

Thomas C. Beam,

Mchale P Bright,

A. C. Pearson

et al.

GeroScience, Journal Year: 2024, Volume and Issue: 46(6), P. 6361 - 6381

Published: June 20, 2024

Language: Английский

Citations

1
An Introductory Guide to Using Bloomington Drosophila Stock Center and FlyBase for Aging Research DOI Creative Commons
Xiangzhong Zheng

Cells, Journal Year: 2024, Volume and Issue: 13(14), P. 1192 - 1192

Published: July 14, 2024

Studies on numerous species have demonstrated strikingly conserved mechanisms that determine the aging process, from yeasts to worms, flies, zebrafish, mice, and humans. The fruit fly Drosophila melanogaster is an excellent model organism for studying biological basis of normal etiology age-related diseases. Since its inception in 1967, Bloomington Stock Center (BDSC) has grown into largest collection documented D. strains (currently > 91,000). This paper aims briefly review provides a guide help users understand organization stock listings BDSC website familiarize themselves with search functions FlyBase, emphasis using genes pathways as examples find stocks studies.

Language: Английский

Citations

1
Decoding lifespan secrets: the role of the gonad in Caenorhabditis elegans aging DOI Creative Commons
André Pires‐daSilva,

Rhianne Kelleher,

Luke Reynoldson

et al.

Frontiers in Aging, Journal Year: 2024, Volume and Issue: 5

Published: March 26, 2024

The gonad has become a central organ for understanding aging in C. elegans , as removing the proliferating stem cells germline results significant lifespan extension. Similarly, when starvation late larval stages leads to quiescence of adult nematode enters reproductive diapause, associated with an extended lifespan. This review summarizes recent advancements identifying mechanisms behind gonad-mediated extension, including comparisons other nematodes and role lipid signaling transcriptional changes. Given that also mediates regulation invertebrates vertebrates, elucidating underlying may help gain new insights into evolution aging.

Language: Английский

Citations

0
Short lifespan is one's fate, long lifespan is one's achievement: lessons from Daphnia DOI Creative Commons

Thomas C. Beam,

Mchale P Bright,

A. C. Pearson

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 30, 2024

Abstract Studies of longevity and senescence rely on baseline life expectancy reference genotypes measured in standardized conditions such as food level group size. Variation lifespan data across labs protocols among can make intervention studies difficult to compare, particularly when GxE interactions exist. Furthermore, extending the a short-lived genotype or any under suboptimal may be lesser theoretical translational value than maximal possible lifespan. Daphnia is rapidly becoming model organism choice for research complementing obtained traditional models. In this study we report several (parthenogenetic clones) long-lived species D. magna variety laboratory protocols, aiming document highest lifespan, factors reducing it, physiological parameters that change with age correlate longevity. Combining from 25 different experiments two strong differences clones geographic origin, moderate effects size medium composition longevity, respect level. Specifically, tend originate small intermittent habitats show little no caloric restriction (CR) extension, while ones expand their even further maintained at 25% ad libitum food. We find evidence trade-offs between fecundity correlations age-specific feeding rate. short-lived, CR non-responsive correlation measures lipid peroxidation (LPO: hydroperoxides MDA abundance). contrast, long-lived, CR-responsive positive hydroperoxide abundance age, negative concentration about median This indicates longevity-related accumulation LPO targets, efficiency detoxification products, and/or Our observations support hypothesis long affected by availability levels oxidative damage, genetically determined short remains regardless. suggest set condition used .

Language: Английский

Citations

0
Elucidating the effective age for dietary restriction and the key metabolites involved DOI Creative Commons

Jazween Loo,

Geetha Gunasekaran, Jen Kit Tan

et al.

Experimental Gerontology, Journal Year: 2024, Volume and Issue: 197, P. 112601 - 112601

Published: Oct. 10, 2024

Dietary restriction (DR) extends lifespan in various species, but its effect at different ages, especially when started later, is unclear. This study used Caenorhabditis elegans to explore the impact of DR ages. Worms were divided into control and groups, with daily survival monitored. To confirm occurrence DR, expression DR-sensitive genes namely acdh-1, pyk-1, pck-2 cts-1 determined using RT-qPCR. Liquid chromatography mass spectrometry (LC-MS) was employed observe changes metabolites affected by DR. The results indicated that young worms subjected mild displayed longest lifespan, highlighting effectiveness initiating a age. Increased acdh-1 suggests activation beta-oxidation gluconeogenesis, while decreased indicates reduced citric acid cycle, further supporting observed effects these worms. Metabolomic activity mechanistic Target Rapamycin (mTOR) synthesis amino acids leucine, tyrosine tryptophan conserve energy for cell repair survival. also levels N-acetyl-L-methionine S-adenosyl-methionine (SAM) methionine metabolism, thereby promoting autophagy, reducing inflammation, facilitating removal damaged cells proteins. In conclusion, dietary early life modulating metabolism enhancing autophagy pathway, maintaining cellular wellbeing.

Language: Английский

Citations

0
Organophospate-Induced Pathology: Mechanisms of Development, Principles of Therapy and Features of Experimental Studies DOI
Н. В. Гончаров, Daria A. Belinskaia, П. В. Авдонин

et al.

Journal of Evolutionary Biochemistry and Physiology, Journal Year: 2023, Volume and Issue: 59(5), P. 1756 - 1796

Published: Sept. 1, 2023

Language: Английский

Citations

1
Organophospate-Induced Pathology: Mechanisms of Development, Principles of Therapy and Features of Experimental Studies DOI
Н. В. Гончаров, Daria A. Belinskaia, П. В. Авдонин

et al.

Российский физиологический журнал им  И  М  Сеченова, Journal Year: 2023, Volume and Issue: 109(10), P. 1349 - 1397

Published: Oct. 1, 2023

Organophosphates (OP) are one of the most common neurotoxic xenobiotics. In acute OP poisoning, as a result suppression synaptic acetylcholinesterase (AChE) activity, cholinergic syndrome develops, which can transform into status epilepticus. Within few days after so-called an intermediate develop, is associated with prolonged inhibition AChE, desensitization nicotinic receptors, and functional degradation synapses muscle fibers. 10–20 single or repeated subacute OP-induced delayed polyneuropathy (OPIDN) develop – neurodegenerative disease, signs ataxia, loss function distal sensory motor axons peripheral nerves. The occurrence neuropsychiatric disorder (NPD) caused by chronic exposure to relatively low-toxicity organophosphorus compounds usually not poisoning; symptoms include cognitive impairment, fatigue, extrapyramidal symptoms. list possible diseases pathological conditions (syndromes) that acute, effects on human body has expanded in recent years due number known (Alzheimer’s, Parkinson’s, multiple sclerosis, etc.). aging general brain particular considered review from point view consequences serve nonspecific trigger related diseases. Gulf consequence intoxication, but also interest context pathology, since its etiology pathogenesis cholinesterase inhibitors. presents data indicating important role vascular endothelium development pathology; first suggestions were made clinicians late 1980s, experimental obtained early 2000s. principles therapy for poisoning outlined, taking account years. Some methods studying experiments vitro, ex vivo laboratory animals, including use carboxylesterase inhibitors, presented. part investigations been remains search new biomarkers assess effectiveness adjuvant regenerative therapies.

Language: Английский

Citations

0