Harnessing lactic acid bacteria for nicotinamide mononucleotide biosynthesis: a review of strategies and future directions DOI Creative Commons

Linghui Kong,

Xinyu Li, Taiyu Liu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 13, 2024

Nicotinamide mononucleotide (NMN), one of the crucial precursors nicotinamide adenine dinucleotide, has garnered considerable interest for its pharmacological and anti-aging effects, conferring potential health economic benefits humans. Lactic acid bacteria (LAB) are most important probiotics, which is commonly used in dairy industry. Due to probiotic properties, it presents an attractive platform food-grade NMN production. LAB have also been extensively utilized enhance functional properties pharmaceuticals cosmetics, making them promising candidates large-scale up synthesis NMN. This review provides in-depth analysis various metabolic engineering strategies, including enzyme optimization, pathway rewiring, fermentation process enhancements, increase yields LAB. It explores both CRISPR/Cas9 traditional methods manipulate key biosynthetic pathways. In particular, this study discussed future research directions, emphasizing application synthetic biology, systems AI-driven optimization further invaluable insights into developing scalable industrially relevant processes production meet growing market demand.

Language: Английский

Whole-cell synthesis of nicotinamide mononucleotide by recombinant Saccharomyces cerevisiae from glucose and nicotinamide DOI
Chaoguang Wang,

Xiaohan Hui,

George Marshall

et al.

Biochemical Engineering Journal, Journal Year: 2024, Volume and Issue: 212, P. 109528 - 109528

Published: Oct. 10, 2024

Language: Английский

Citations

4
Microbial Production of Nicotinamide Mononucleotide: Key Enzymes Discovery, Host Cells Selection, and Pathways Design and Optimization DOI
Xing Shen,

Xiulong Kang,

Rui Wang

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

As an important bioactive substance in cells, nicotinamide mononucleotide (NMN) has been proven to play role antiaging, treatment of neurodegenerative diseases, and cardioprotection. It presents a high potential for application the research fields functional foods, cosmetics, healthcare products, active pharmaceuticals. With increased demand, whether NMN can achieve large-scale industrial production wide concern. The chemical synthesis method mainly faces problems separation, purification, complex process control; contrast, biosynthesis methods such as microbial fermentation enzyme catalysis are considered be mainstream future due advantages environmental friendliness, efficiency, simple separation. This review first describes physiological functions related areas its applications. Subsequently, it focuses on progress different synthetic pathways biosynthetic approaches, mining modification key enzymes, chassis cell design optimization, whole-cell catalysis. Meanwhile, regulatory strategies, methods, control also elaborated, efficiencies cells systematically compared. Finally, this summarizes existing challenges proposes strategies directions address these issues. work provides technical references theoretical basis researching efficient application.

Language: Английский

Citations

0
An overview of engineering microbial production of nicotinamide adenine mononucleotide DOI

Baoxian Li,

Xiangfeng Meng, Weifeng Liu

et al.

Journal of Biotechnology, Journal Year: 2024, Volume and Issue: 396, P. 80 - 88

Published: Nov. 2, 2024

Language: Английский

Citations

1
Systematic Engineering for High-level Production of β-nicotinamide mononucleotide from NAM and Ribose DOI Creative Commons
T. W. Bao, P.S. Weng, Jingfang Wang

et al.

Food Bioscience, Journal Year: 2024, Volume and Issue: unknown, P. 105725 - 105725

Published: Dec. 1, 2024

Language: Английский

Citations

1
De Novo Biosynthesis of L-Azetidine-2-Carboxylic Acid in Escherichia coli Strains for Powdery Mildew Treatment DOI
Jie Zheng,

Hongxiang Liu,

Jiwen Zhang

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

L-Azetidine-2-carboxylic acid (L-Aze), a natural nonproteinogenic amino found widely in plants, has recently been identified as an environmentally friendly agent for controlling powdery mildew with low toxicity. In this study, biological route L-Aze production via the methionine salvage pathway (Yang Cycle) was first

Language: Английский

Citations

0
Harnessing lactic acid bacteria for nicotinamide mononucleotide biosynthesis: a review of strategies and future directions DOI Creative Commons

Linghui Kong,

Xinyu Li, Taiyu Liu

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 13, 2024

Nicotinamide mononucleotide (NMN), one of the crucial precursors nicotinamide adenine dinucleotide, has garnered considerable interest for its pharmacological and anti-aging effects, conferring potential health economic benefits humans. Lactic acid bacteria (LAB) are most important probiotics, which is commonly used in dairy industry. Due to probiotic properties, it presents an attractive platform food-grade NMN production. LAB have also been extensively utilized enhance functional properties pharmaceuticals cosmetics, making them promising candidates large-scale up synthesis NMN. This review provides in-depth analysis various metabolic engineering strategies, including enzyme optimization, pathway rewiring, fermentation process enhancements, increase yields LAB. It explores both CRISPR/Cas9 traditional methods manipulate key biosynthetic pathways. In particular, this study discussed future research directions, emphasizing application synthetic biology, systems AI-driven optimization further invaluable insights into developing scalable industrially relevant processes production meet growing market demand.

Language: Английский

Citations

0